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Int. J. Mol. Sci. 2013, 14(7), 14518-14531; doi:10.3390/ijms140714518

Exploiting CRISPR/Cas: Interference Mechanisms and Applications

Prokaryotic Small RNA Biology, Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Straße 10, 35043 Marburg, Germany
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Received: 31 May 2013 / Revised: 26 June 2013 / Accepted: 1 July 2013 / Published: 12 July 2013
(This article belongs to the Special Issue Regulation by non-coding RNAs 2013)
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Abstract

The discovery of biological concepts can often provide a framework for the development of novel molecular tools, which can help us to further understand and manipulate life. One recent example is the elucidation of the prokaryotic adaptive immune system, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) that protects bacteria and archaea against viruses or conjugative plasmids. The immunity is based on small RNA molecules that are incorporated into versatile multi-domain proteins or protein complexes and specifically target viral nucleic acids via base complementarity. CRISPR/Cas interference machines are utilized to develop novel genome editing tools for different organisms. Here, we will review the latest progress in the elucidation and application of prokaryotic CRISPR/Cas systems and discuss possible future approaches to exploit the potential of these interference machineries. View Full-Text
Keywords: CRISPR; crRNA; Cas9; Cascade; interference; genome editing; RGEN; TALEN; ZNF CRISPR; crRNA; Cas9; Cascade; interference; genome editing; RGEN; TALEN; ZNF
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Richter, H.; Randau, L.; Plagens, A. Exploiting CRISPR/Cas: Interference Mechanisms and Applications. Int. J. Mol. Sci. 2013, 14, 14518-14531.

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