Characterization of a Gene Encoding Clathrin Heavy Chain in Maize Up-Regulated by Salicylic Acid, Abscisic Acid and High Boron Supply
Abstract
:1. Introduction
2. Results
2.1. Cloning and Characterization of Genes ZmCHC1 and ZmCHC2 Encoding Clathrin Heavy Chain in Zea Mays
2.2. Sequence Analysis of ZmCHC1 and ZmCHC2
2.3. Organ Specific Expression Patterns of ZmCHC1 and ZmCHC2 in Maize Seedling
2.4. Effect of ABA, High Boron Supply, Sodium Azide or Low Temperature on Expressions of ZmCHC1 and ZmCHC2 in Maize Plants
2.5. Salicylic Acid-Inducible Expression of ZmCHC1 Gene in Maize Roots
3. Discussion
4. Experimental Section
4.1. Maize Plants and Growth Conditions
4.2. Salicylic Acid, Abscisic Acid, High Boron, Sodium Azide, Tyrphostin A23, or Low Temperature Treatment of Maize Plants
4.3. Molecular Cloning of CHC Homolog from Maize
4.4. Sequence Analyses and Alignments
4.5. Real-Time Quantitative RT-PCR Performance
5. Conclusions
Acknowledgments
Conflict of Interest
References
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cis-Acting elements | Position (upstream of the start codon of ZmCHC1 or ZmCHC2) | Core sequence | Function | ||
---|---|---|---|---|---|
ZmCHC1 | ZmCHC2 | ZmCHC1 | ZmCHC2 | ||
TCA-element | −403 to −412 | GAGAAGAAGA | Salicylic acid responsiveness | ||
ABRE | −152 to −143 | −880 to −875 | CCGACGTGGC | CACGTG | Abscisic acid responsiveness |
−1163 to −1158 | CGTGGA | ||||
−1189 to −1180 | TGGGGGTGGC | ||||
−1441 to −1436 | TACGTG | ||||
Box-W1 | −1470 to −1475 | −1279 to −1274 | TTGACC | TTGACC | Fungal elicitor responsiveness |
TGACG-motif | −66 to −70 −847 to −843 | −945 to −949 | TGACG TGACG | TGACG | Methyl jasmonate responsiveness |
−1065 to −1069 | TGACG | ||||
−1245 to −1241 | TGACG |
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Zeng, M.-H.; Liu, S.-H.; Yang, M.-X.; Zhang, Y.-J.; Liang, J.-Y.; Wan, X.-R.; Liang, H. Characterization of a Gene Encoding Clathrin Heavy Chain in Maize Up-Regulated by Salicylic Acid, Abscisic Acid and High Boron Supply. Int. J. Mol. Sci. 2013, 14, 15179-15198. https://doi.org/10.3390/ijms140715179
Zeng M-H, Liu S-H, Yang M-X, Zhang Y-J, Liang J-Y, Wan X-R, Liang H. Characterization of a Gene Encoding Clathrin Heavy Chain in Maize Up-Regulated by Salicylic Acid, Abscisic Acid and High Boron Supply. International Journal of Molecular Sciences. 2013; 14(7):15179-15198. https://doi.org/10.3390/ijms140715179
Chicago/Turabian StyleZeng, Mu-Heng, Sheng-Hong Liu, Miao-Xian Yang, Ya-Jun Zhang, Jia-Yong Liang, Xiao-Rong Wan, and Hong Liang. 2013. "Characterization of a Gene Encoding Clathrin Heavy Chain in Maize Up-Regulated by Salicylic Acid, Abscisic Acid and High Boron Supply" International Journal of Molecular Sciences 14, no. 7: 15179-15198. https://doi.org/10.3390/ijms140715179