Fumaric Acid Esters Do Not Reduce Inflammatory NF-κB/p65 Nuclear Translocation, ICAM-1 Expression and T-Cell Adhesiveness of Human Brain Microvascular Endothelial Cells
Abstract
:1. Introduction
2. Results
2.1. FAE Do Not Modulate NF-κB/p65 Nuclear Translocation or p38 MAPK Activation in HBMEC
2.2. FAE Do Not Reduce Basal or Inflammatory-Inducible Expression of ICAM-1 in HBMEC
2.3. FAE Do Not Reduce T-Cell Adhesiveness of HBMEC
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Stimulation
4.3. Cell Protein Extracts and Western Blotting
4.4. Flow Cytometry
4.5. Adhesion Assay
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Haarmann, A.; Nehen, M.; Deiß, A.; Buttmann, M. Fumaric Acid Esters Do Not Reduce Inflammatory NF-κB/p65 Nuclear Translocation, ICAM-1 Expression and T-Cell Adhesiveness of Human Brain Microvascular Endothelial Cells. Int. J. Mol. Sci. 2015, 16, 19086-19095. https://doi.org/10.3390/ijms160819086
Haarmann A, Nehen M, Deiß A, Buttmann M. Fumaric Acid Esters Do Not Reduce Inflammatory NF-κB/p65 Nuclear Translocation, ICAM-1 Expression and T-Cell Adhesiveness of Human Brain Microvascular Endothelial Cells. International Journal of Molecular Sciences. 2015; 16(8):19086-19095. https://doi.org/10.3390/ijms160819086
Chicago/Turabian StyleHaarmann, Axel, Mathias Nehen, Annika Deiß, and Mathias Buttmann. 2015. "Fumaric Acid Esters Do Not Reduce Inflammatory NF-κB/p65 Nuclear Translocation, ICAM-1 Expression and T-Cell Adhesiveness of Human Brain Microvascular Endothelial Cells" International Journal of Molecular Sciences 16, no. 8: 19086-19095. https://doi.org/10.3390/ijms160819086