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Article

Gallic Acid Alleviates Hypertriglyceridemia and Fat Accumulation via Modulating Glycolysis and Lipolysis Pathways in Perirenal Adipose Tissues of Rats Fed a High-Fructose Diet

by
Da-Wei Huang
1,
Wen-Chang Chang
2,
Heng-Jui Yang
3,
James Swi-Bea Wu
2 and
Szu-Chuan Shen
3,*
1
Department of Food and Beverage Management, China University of Science and Technology, No. 245, Sec. 3, Academia Road, Taipei 11581, Taiwan
2
Graduate Institute of Food Science and Technology, National Taiwan University, P.O. Box 23-14, Taipei 10672, Taiwan
3
Department of Human Development and Family Studies, National Taiwan Normal University, No. 162, Sec. 1, Heping East Road, Taipei 10610, Taiwan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(1), 254; https://doi.org/10.3390/ijms19010254
Submission received: 20 November 2017 / Revised: 12 January 2018 / Accepted: 13 January 2018 / Published: 15 January 2018
(This article belongs to the Section Bioactives and Nutraceuticals)
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Abstract

:
This study investigated the ameliorative effect of gallic acid (GA) on hypertriglyceridemia and fat accumulation in perirenal adipose tissues of high-fructose diet (HFD)-induced diabetic rats. The previous results showed that orally administered GA (30 mg/kg body weight) for four weeks significantly reduced the levels of plasma glucose and triglyceride (TG) in HFD rats. GA also markedly decreased the perirenal adipose tissues weight of HFD rats in present study (p < 0.05). Western blot assay indicated that GA restored expression of insulin signaling-related proteins, such as insulin receptor (IR), protein kinase C-zeta (PKC-ζ), and glucose transporter-4 (GLUT4) in the perirenal adipose tissues of HFD rats. Moreover, GA enhanced expression of glycolysis-related proteins, such as phosphofructokinase (PFK) and pyruvate kinase (PK), and increased the expression of lipolysis-related proteins, such as adipose triglyceride lipase (ATGL), which is involved in lipolysis in the perirenal adipose tissues of HFD rats. This study revealed that GA may alleviate hypertriglyceridemia and fat accumulation through enhancing glycolysis and lipolysis pathways in perirenal adipose tissues of HFD rats. These findings also suggest the potential of GA in preventing the progression of diabetes mellitus (DM) complications.

Graphical Abstract

1. Introduction

Diabetes mellitus (DM) is one of the fastest growing chronic diseases and is caused by a deficiency in insulin secretion or by ineffectiveness in insulin action [1]. Dysfunction of insulin requirements is involved in the derangement of carbohydrate, protein, and lipid metabolism, leading to numerous complications, such as hyperlipidemia, coronary artery disease, renal failure, neuropathy, retinopathy, and blindness [1,2]. More than 95% of diabetic patients have type 2 DM (T2DM), causing hyperglycemia via an ineffectiveness of insulin action or an inability to mount a normal response to insulin in peripheral cells, resulting in the symptoms of insulin resistance [3,4]. Fructose can cause hypertriglyceridemia, low density lipoprotein-cholesterol (LDL-C), weight gain, blood pressure elevation, and impaired glucose tolerance [5]. Long-term fructose intake may enhance oxidative stress, impair the antioxidant system, and increase lipid peroxidation [6,7]. It can also be associated with the pathogenesis of metabolic syndrome, including insulin resistance, abdominal obesity, dyslipidemia, intraabdominal fat accumulation, fatty liver, inflammation, and endothelial dysfunction, resulting in T2DM ultimately [5]. High-fructose diet (HFD)-induced diabetic rats are widely used as an in vivo model to investigate the mechanism of therapy for T2DM-associated insulin resistance [8].
Phenolic compounds are widely distributed in the plant kingdom. Plant-derived polyphenol compounds exhibit various pharmacological properties, which has been the subject of considerable interest in recent research [4]. Gallic acid (GA), an endogenous polyphenol in plants, is abundant in vegetables, grapes, berries, tea, fruit juices, and wine [1]. GA consists of one aromatic ring, three hydroxyl groups, and one carboxylic acid group. GA exhibits the strong antioxidant capacity due to the fact that three hydroxyl groups are linked to the aromatic ring in the ortho position. GA has been reported to exhibit pharmacological activities, including antioxidant, anti-obesity, anti-inflammatory, antimutagenic, and anticancer activity [9,10]. Moreover, GA exhibits antihyperglycemic, anti-lipid peroxidative, and antioxidant activities in streptozotocin (STZ)-induced diabetic rats [11]. In these rats, the oral treatment with GA resulted in a significant decrease in the levels of blood glucose, hepatic lipid peroxidation products, glycoprotein components, lipids and the activity of hydroxymethylglutaryl-CoA reductase, and a significant increase in levels of plasma insulin and liver glycogen [11].
An HFD-induced diabetic rat model has been reported to present the pathophysiological properties of T2DM in humans such as insulin resistance, glucose intolerance, dyslipidemia, renal impairment, and hypertension [12]. High fructose intake is linked to the prevalence of hyperglycemia, hypertriglyceridemia, obesity, and other metabolic syndromes [8]. Very few studies have examined the effect of GA on fat accumulation in adipose tissues of diabetes. The perirenal adipose tissue is the relatively large size in the intra-abdominal cavity and facilitates to cause a mass increase when compared with other adipose tissue [13]. The aim of the present study is to investigate the effect of GA on hypertriglyceridemia and fat accumulation in perirenal adipose tissues of HFD-induced diabetic rats.

2. Results

2.1. Effect of GA on Weight of Perirenal and Epidydimal Adipose Tissues in HFD-Induced Diabetic Rats

Perirenal and epididymal adipose tissue from rats was acquired and weighed after sacrifice. The results indicated that HFD increased perirenal and epidydimal adipose weight by 71.6% and 84.5% in comparison to the normal group, respectively. However, administration of 10 mg/kg body weight GA diminished the weight of perirenal and epidydimal adipose by 32.3% and 44.2% in HFD rats (p < 0.05), treatment of 30 mg/kg body weight GA caused 31.7%, and 54.1% decrease in HFD rats (p < 0.05) (Figure 1).

2.2. Effect of GA on Insulin Signal Transduction in the Perirenal Fat of HFD Rats

Figure 2 shows that HFD significantly decreased the expression of IR by 67.1% in normal rats. Administration of 10 or 30 mg/kg body weight GA increased IR expression by 18.9% and 51.5% in HFD rats, respectively (p < 0.05) (Figure 2A). HFD also led to a 34.5% decrease in GLUT4 expression in the normal rats (p < 0.05) (Figure 2A). Administration of 10 or 30 mg/kg body weight GA remarkably restored the expression of GLUT4 by 70.5% and 22.3% when compared with the negative control group (p < 0.05) (Figure 2A). The expression of PKC-ζ declined by 29.3% in HFD rats compared with the normal group (p < 0.05) (Figure 2B). Administration of 10 or 30 mg/kg body weight GA enhanced PKC-ζ expression by 71.3% and 86.6%, respectively, in HFD rats (p < 0.05) (Figure 2B).

2.3. Effect of GA on Carbohydrate Metabolism and Lipid Metabolism in the Perirenal Fat of HFD Rats

HFD rats exhibited a 49.4% decrease in phosphofructokinase (PFK) expression as compared with the normal group (p < 0.05) (Figure 3A). Treatment with 10 or 30 mg/kg body weight GA significantly increased PFK expression by 208.3% and 99.6%, respectively, in HFD animals (p < 0.05) (Figure 3A). The expression of pyruvate kinase (PK) was suppressed by 80.0% in this group (p < 0.05) (Figure 3A). HFD rats administered 10 and 30 mg/kg body weight GA had restored PK expression, by 91.1% and 83.7%, respectively (p < 0.05) (Figure 3A). The rats fed with HFD also had a 71.6% decrease in expression of adipose triglyceride lipase (ATGL), which was enhanced by 171.6% in HFD rats treated with 30 mg/kg body weight GA (p < 0.05) (Figure 3B).

3. Discussion

The antioxidant propriety of GA is demonstrated to prevent the progression of diabetic complications [14]. GA is also reported to exhibit antihyperglycemic, anti-lipid peroxidative, and antioxidant effects on STZ-induced diabetic rats [11], and to ameliorate impaired glucose and lipid homeostasis in HFD-induced non-alcoholic fatty liver disease mice [9]. Our previous study indicated that GA ameliorates hyperglycemia and improves hepatic carbohydrate metabolism in HFD rats. The present study is to evaluate the effect of GA on amelioration of insulin signal transduction and lipid and glucose metabolism in the perirenal adipose tissues of HFD-induced diabetic rats.
HFD rats have been widely used as models for evaluating insulin resistance as well as diabetes [7,8]. High fructose intake may cause hyperglycemia, increase oxidative stress, and decrease insulin sensitivity, leading to insulin resistance in liver, skeletal muscle, and adipose tissues [15]. The previous study illustrated that GA significantly increased the ability of glucose uptake in hepatocytes and lead to alleviate hyperglycemia in HFD rats [7]. In this study, we proposed that enhancing the glucose uptake in adipocytes may be also associated with the advantage of GA on decreasing plasma glucose in HFD rats.
High fructose dietary may promote de novo lipogenesis and cause the accumulation of endogenous TGs, resulting in hyperlipidemia [15]. Phenolic acid was previously verified to improve abnormal lipid metabolism via reducing serum TG and LDL-C levels and enhancing HDL-C levels in diabetic rats [1]. The results from the current study indicate that GA supplementation may cause a significant reduction in serum TG levels in HFD rats. A previous study indicated that the administration of HFD causes an increase in intra-abdominal adipose mass in rats [13]. Hsu et al. illustrated that GA decreases epidydimal and perirenal fat weight in HFD-induced diabetic animals [16]. The present study revealed that the administration of GA might significantly decrease epidydimal and perirenal adipose weight in HFD rats, which is consistent with these previous findings. Treatment with GA was reported to improve lipid profiles by inhibiting lipogenesis in the adipose tissue of diabetic rats [17]. Okuno et al. verified that administration of troglitazone may decrease the concentration of triglyceride and free fatty acids in the blood via increasing number of small adipocytes in white adipose tissue, resulting in the amelioration of insulin resistance [18]. GA also induces apoptosis in 3T3-L1 pre-adipocytes, resulting in decreasing pre-adipocyte proliferation [19]. We speculate that administration of GA may reduce perirenal fat accumulation by suppressing adipocytes proliferation, leading to a decrease in perirenal adipose tissues weight and plasma triglyceride level in HFD rats.
In normal conditions, insulin binds to IR, triggers tyrosine phosphorylation of IR, and subsequently enhances the expression of IR substrate (IRS), phosphatidylinositol-3-kinase (PI3K), and Akt/protein kinase B (PKB), thus inducing translocation of GLUT to the cell membrane for promoting uptake of glucose in peripheral tissues [20]. GLUT4 is the predominant GLUT in adipocytes and responsible for glucose uptake via activation of the PI3K-Akt (PKB) or PI3K-protein kinase C (PKC) pathway [21]. GA was reported to cause translocation and activation of GLUT4 through boosting PI3K activity and phosphorylation of Akt in adipose tissue of high-fat diet fed-streptozotocin-induced rats [22]. Furthermore, the ζ isotype of protein kinase C (PKC-ζ) is a member of the atypical PKC subfamily, which has been widely recognized as a key regulator of critical intracellular signaling pathways and stimulated the action of GLUT4 [23,24]. GA is also reported to enhance the expression of GLUT4 via activating the PI3K-PKC pathway in 3T3-L1 adipocytes, resulting in an increased glucose uptake [24]. Previous studies have indicated that the expression of IR, insulin receptor substrate 1 (IRS-1), PI3K, and Akt is decreased in the muscle tissue of HFD-induced diabetic rats [25]. In the current study, administration of GA improved impaired protein expression of GLUT4 via increased protein expression of IR and PKC-ζ in perirenal fat, suggesting that GA may ameliorate insulin-signaling cascades through the PI3K-PKC pathway in HFD-induced diabetic rats.
Glycolysis is the first step in glucose metabolism and use. Earlier studies demonstrated that the activity of glycolysis-related enzymes is diminished in T2DM rats [4]. PFK and PK play roles as the rate-limiting enzyme and the final step of glycolysis, respectively, and are mainly modulated by insulin [26,27]. Impaired expression of PFK may be improved by metformin in skeletal muscle, liver, and adipose tissues of STZ-induced diabetic mice [26]. PK activity and mRNA expression are decreased in adipose tissue of type 1 DM patients and in an insulin-deficient diabetic animal model [28]. Previous studies indicated that insulin ameliorates the activity and expression of hepatic PK, leading to ameliorated hyperglycemia in alloxan monohydrate-treated rats [29]. Here, we found that GA may increase expressions of PFK and PK via improving insulin sensitivity, resulting in improving glycolysis and subsequently ameliorating glucose metabolism in the perirenal adipose tissues of HFD diabetic rats.
Adipocytes reduce lipolysis may cause maximizing triglyceride packaging into lipid droplets of adipocytes [30]. Ruegsegger et al. indicated that perirenal adipose tissue is the relatively large size in the intra-abdominal cavity [13]. In this study, we evaluated the ameliorative effect of GA on fat accumulation in perirenal adipose tissues in HFD rats. Dysfunctional lipolysis may influence energy homeostasis and the pathogenesis of obesity and insulin resistance [31]. Obesity is characterized by an increase in TG storage in adipose tissue [32]. ATGL is a key enzyme that is involved in intracellular degradation of triacylglycerol and is highly expressed in white and brown adipose tissue of mice and humans [33]. Earlier work showed that mRNA and protein expression of ATGL is reduced in the insulin-resistant state [32]. GA was reported to cause a significant decrease in triacylglycerol levels in HFD rats [7]. In the present study, the administration of 30 mg/kg body weight GA appeared to ameliorate the impaired ATGL expression in the perirenal fat of HFD diabetic rats. We speculated that GA may decrease the pathogenesis of obesity via promoting lipolysis in the HFD rat perirenal adipose tissues. We hypothesized that GA may ameliorate impaired insulin signal transduction and enhance glucose utilization via improving insulin sensitivity, and modulate lipid metabolism by the enhancement of ATGL expression, resulting in decreasing hyperglycemia and fat accumulation in perirenal adipose tissues of HFD rats as the consequence (Figure 4).
Oral administration of GA at a dose of 500 mg/kg was demonstrated to cause no toxicity or mortality in mice. GA is non-toxic at an acute dose of 5000 mg/kg body weight; this dose is recognized as no-observed-adverse-effect level (NOAEL) for GA in mice. A subacute administration of 1000 mg/kg body weight also confirmed its safety [34]. Subchronic toxicity of GA was investigated that the level of 119 and 128 mg/kg body weight/day were determined to be the NOAEL in male and female rats, respectively [35]. These levels of GA represent human equivalent doses of 19.4 and 20.48 mg in a 60 kg male and female in humans, respectively, as described by the FDA guidance for industry on conversion from rat to human equivalent doses [36]. Hence, we speculated that the administration of 30 mg/kg body weight GA (4.8 mg/kg body weight GA for human) has a high potential with the least toxicity in this study.

4. Materials and Methods

4.1. Chemicals

GA (purity > 97.5%, Analytical grade), Glucose Assay Kit, D-Glucose, fructose, pioglitazone hydrochloride, Tris-HCl, Triton X-100, and PMSF were purchased from Sigma (St. Louis, MO, USA). Anti-IR, anti-PKC-ζ, anti-GLUT4, anti-PFK, anti-PK, and anti-ATGL antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). All of the chemicals used in this study were of analytical grade.

4.2. Animals and Diets

Male Wistar rats (6 weeks old) were obtained from the National Laboratory Animal Center, Taipei, Taiwan. The room conditions and treatment procedures were in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animal. All of the protocols were approved by the Institutional Animal Care and Use Committee of National Taiwan Normal University, Taipei, Taiwan (approval No. 103042, 23 December 2014). The rats were maintained under standard laboratory conditions of temperature 25 °C ± 1 °C and a 12-h light/12-h dark cycle with free access to food and water for the duration of the study. Wistar rats were fed a normal diet for two weeks and divided into five groups of six rats each. Group 1 was fed a normal diet for eight weeks. Group 2 was fed HFD throughout the experiment as a negative control. Group 3 received HFD for 8 weeks and was gavaged with pioglitazone (30 mg/kg) daily for the last four weeks as the positive control. Groups 4 and 5 were fed HFD (66% fructose in diet) for 8 weeks and gavaged with GA (10 or 30 mg/kg body weight, respectively) daily for the last four weeks of the eight-week period. The ingredients composition of experimental diets is according to our previous study [7].

4.3. Western Blot Analysis

The perirenal fat pad (1 g) was homogenized with 3 mL of RIPA buffer solution (ddw 8.7 mL/10× RIPA buffer 1 mL/100 mM PMSF 100 µL/Phosphatase Inhibitor Cocktail2 100 µL/Phosphatase Inhibitor Cocktail3 100 µL) at 4 °C for 10 min. The homogenate was centrifuged (3000 rpm, 5 min, 4 °C) to obtain the liquid at the bottom, which was centrifuged (15,000× g, 20 min, 4 °C) to acquire the cell protein for western blot analysis.
Aliquots of the extract, each containing 40 μg of protein, were evaluated for the expression of IR, GLUT4, PKC-ζ, PFK, PK, and ATGL. The samples were subjected to 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The separated proteins were electrotransferred to a polyvinylidene difluoride membrane that was incubated with blocking buffer (phosphate-buffered saline containing 0.05% Tween-20 (PBST) and 5% w/v nonfat dry milk) for 1 h, washed three times with PBST, and then probed with 1:1000–1:2000 diluted solutions of anti-IR, anti-GLUT4, anti-PKC-ζ, anti-PFK, anti-PK, and anti-ATGL at 4 °C overnight. The intensity of the blots probed with 1:2000 diluted solutions of anti-α-tublin was used as a control to ensure that a constant amount of protein was loaded into each lane of the gel. The membrane was washed three times for 5 min each in PBST, and then incubated in a solution of horseradish peroxidase-conjugated anti-mouse IgG or anti-rabbit IgG secondary antibody, washed three times again for 5 min each in PBST, and exposed to the enhanced chemiluminescence reagent (Millipore, Darmstadt, Germany). Autoradiography was scanned and analyzed using a UVP Biospectrum image system (Level, Cambridge, UK).

4.4. Statistical Analysis

Data are presented as means ± SD after analysis using one-way ANOVA and Duncan’s new multiple-range tests with the Statistical Analysis System (SAS) version 9.3 (Cary, NC, USA). All of the comparisons were made relative to the controls or negative control. The differences were considered to be statistically significant at p < 0.01 or 0.05, indicated in tables and figures by distinct letters.

5. Conclusions

The present study demonstrates that administration of GA may be helpful in ameliorating carbohydrate and lipid metabolism abnormalities via restoring insulin signaling, increasing glycolysis and lipolysis-related protein expressions, which reduces fat accumulation in perirenal adipose tissues and alleviate hypertriglyceridemia in HFD-induced diabetic rats. Our findings support that GA exerts therapeutic effects and has the potential to be used in clinical medicine or as a dietary supplement on preventing the progression of complications in DM.

Acknowledgments

The authors would like to thank the Ministry of Science and Technology of the Republic of China, Taiwan, for mainly financially supporting this research under contract No. MOST 104-2320-B-003-003-MY3. This article was also partially subsidized by the National Taiwan Normal University (NTNU), Taiwan.

Author Contributions

Szu-Chuan Shen designed the trial, analyzed the data and wrote the manuscript. Da-Wei Huang and Heng-Jui Yang participated in the design of the trial, mainly conducted data analysis and interpretation of data and participated in manuscript writing. Wen-Chang Chang and James Swi-Bea Wu participated in the design of the trial, conducted the research and participated in the interpretation of data and manuscript writing.

Conflicts of Interest

The authors declare no conflict of interest.

Abbreviations

ATGLAdipose triglyceride lipase
DGDiglyceride
DMDiabetes mellitus
F-1,6-BPFructose 1,6 biphosphate
FFAFree fatty acid
GAGallic acid
GLUT4Glucose transporter-4
HFDHigh-fructose diet
IRInsulin receptor
IRSIR substrate
IRS-1IR substrate 1
LDL-CLow density lipoprotein-cholesterol
NOAELNo-observed-adverse-effect level
PFKPhosphofructokinase
PI3KPhosphatidylinositol-3-kinase
PKPyruvate kinase
PKBAkt/protein kinase B
PKCProtein kinase C
PKC-ζProtein kinase C-zeta
STZStreptozotocin
T2DMType 2 DM
TGTriglyceride

References

  1. Latha, R.C.R.; Daisy, P. Insulin-secretagogue, antihyperlipidemic and other protective effects of gallic acid isolated from Terminalia bellerica Roxb. in streptozotocin-induced diabetic rats. Chem. Biol. Interact. 2011, 189, 112–118. [Google Scholar] [CrossRef] [PubMed]
  2. Huang, D.W.; Chang, W.C.; Wu, J.S.B.; Shih, R.W.; Shen, S.C. Vescalagin from Pink wax apple [Syzygium samarangense (Blume) Merrill and Perry] alleviates hepatic insulin resistance and ameliorates glycemic metabolism abnormality in rats fed a high-fructose diet. J. Agric. Food Chem. 2016, 57, 7687–7692. [Google Scholar] [CrossRef] [PubMed]
  3. Björnholm, M.; Zierath, J.R. Insulin signal transduction in human skeletal muscle: Identifying the defects in type II diabetes. Biochem. Soc. Trans. 2005, 33, 354–357. [Google Scholar] [CrossRef] [PubMed]
  4. Punithavathi, V.R.; Stanely Mainzen Prince, P.; Kumar, M.R.; Selvakumari, C.J. Protective effects of gallic acid on hepatic lipid peroxide metabolism, glycoprotein components and lipids in streptozotocin-induced type II diabetic Wistar rats. J. Biochem. Mol. Toxicol. 2011, 25, 68–76. [Google Scholar] [CrossRef] [PubMed]
  5. Johnson, R.J.; Perez-Pozo, S.E.; Sautin, Y.Y.; Manitius, J.; Sanchez-Lozada, L.G.; Feig, D.I.; Shafiu, M.; Segal, M.; Glassock, R.J.; Shimada, M.; et al. Hypothesis: Could excessive fructose intake and uric acid cause type 2 diabetes? Endocr. Rev. 2009, 30, 96–116. [Google Scholar] [CrossRef] [PubMed]
  6. Collison, K.S.; Saleh, S.M.; Bakheet, R.H.; Al-Rabiah, R.K.; Inglis, A.L.; Makhoul, N.J. Diabetes of the liver: The link between nonalcoholic fatty liver disease and HFCS-55. Obesity 2009, 17, 2003–2013. [Google Scholar] [CrossRef] [PubMed]
  7. Huang, D.W.; Chang, W.C.; Wu, J.S.B.; Shih, R.W.; Shen, S.C. Gallic acid ameliorates hyperglycemia and improves hepatic carbohydrate metabolism in rats fed a high-fructose diet. Nutr. Res. 2016, 36, 150–160. [Google Scholar] [CrossRef] [PubMed]
  8. Lin, S.; Yang, Z.; Liu, H.; Tang, L.; Cai, Z. Beyond glucose: Metabolic shifts in responses to the effects of oral glucose tolerance test and the high-fructose diet in rats. Mol. Biosyst. 2011, 7, 1537–1548. [Google Scholar] [CrossRef] [PubMed]
  9. Chao, J.; Huo1, T.I.; Cheng, H.Y.; Tsai, J.C.; Liao, J.W.; Lee, M.S.; Qin, X.M.; Hsieh, M.T.; Pao, L.H.; Peng, W.H. Gallic acid ameliorated impaired glucose and lipid homeostasis in high fat diet-induced NAFLD mice. PLoS ONE 2014, 9, 1–16. [Google Scholar] [CrossRef] [PubMed]
  10. Umadevi, S.; Gopi, V.; Elangovan, V. Regulatory mechanism of gallic acid against advanced glycation end products induced cardiac remodeling in experimental rat. Chem. Biol. Interact. 2014, 208, 28–36. [Google Scholar] [CrossRef] [PubMed]
  11. Punithavathi, V.R.; Stanely Mainzen Prince, P.; Kumar, M.R.; Selvakumari, C.J. Antihyperglycaemic, antilipid peroxidative and antioxidant effects of gallic acid on streptozotocin induced diabetic Wistar rats. Eur. J. Pharmacol. 2011, 650, 465–471. [Google Scholar] [CrossRef] [PubMed]
  12. Patel, J.; Iyer, A.; Brown, L. Evaluation of the chronic complications of diabetes in a high fructose diet in rats. Indian J. Biochem. Biophys. 2009, 46, 66–72. [Google Scholar] [PubMed]
  13. Ruegsegger, G.N.; Company, J.M.; Toedebusch, R.G.; Roberts, C.K.; Roberts, M.D.; Booth, F.W. Rapid alterations in perirenal adipose tissue transcriptomic networks with cessation of voluntary running. PLoS ONE 2015, 10, 1–22. [Google Scholar] [CrossRef] [PubMed]
  14. Oliveiraa, L.S.; Thoméa, G.R.; Lopesa, T.F.; Reicherta, K.P.; Oliveiraa, J.S.; Pereiraa, A.S.; Baldissarelia, J.; Krewerb, C.C.; Morscha, V.M.; Chitolina Schetinger, M.R.; et al. Effects of gallic acid on delta—Aminolevulinic dehydratase activity and in the biochemical, histological and oxidative stress parameters in the liver and kidney of diabetic rats. Biomed. Pharmacother. 2016, 84, 1291–1299. [Google Scholar] [CrossRef] [PubMed]
  15. Samuel, V.T. Fructose induced lipogenesis: From sugar to fat to insulin resistance. Trends Endocrinol. Metab. 2011, 22, 60–65. [Google Scholar] [CrossRef] [PubMed]
  16. Hsu, J.H.; Yen, G.C. Effect of gallic acid on high fat diet-induced dyslipidaemia, hepatosteatosis and oxidative stress in rats. Br. J. Nutr. 2007, 98, 727–735. [Google Scholar] [CrossRef] [PubMed]
  17. Patel, S.S.; Goyal, R.K. Cardioprotective effects of gallic acid in diabetes-induced myocardial dysfunction in rats. Pharmacogn. Res. 2011, 3, 239–245. [Google Scholar]
  18. Okuno, A.; Tamemoto, H.; Tobe, K.; Ueki, K.; Mori, Y.; Iwamoto, K.; Umesono, K.; Akanuma, Y.; Fujiwara, T.; Horikoshi, H.; et al. Troglitazone increases the number of small adipocytes without the change of white adipose tissue mass in obese Zucker rats. J. Clin. Investig. 1998, 101, 1354–1361. [Google Scholar] [CrossRef] [PubMed]
  19. Hsu, C.L.; Lo, W.H.; Yen, G.C. Gallic acid induces apoptosis in 3T3-L1 pre-adipocytes via a Fas- and mitochondrial-mediated pathway. J. Agric. Food Chem. 2007, 55, 7359–7365. [Google Scholar] [CrossRef] [PubMed]
  20. Saltiel, A.R.; Kahn, C.R. Insulin signaling and the regulation of glucose and lipid metabolism. Nature 2001, 414, 799–806. [Google Scholar] [CrossRef] [PubMed]
  21. Eriksson, J.W. Metabolic stress in insulin’s target cells leads to ROS accumulation—A hypothetical common pathway causing insulin resistance. FEBS Lett. 2007, 581, 3734–3742. [Google Scholar] [CrossRef] [PubMed]
  22. Gandhi, G.R.; Jothi, G.; Antony, P.J.; Balakrishna, K.; Paulraj, M.G.; Ignacimuthu, S.; Stalin, A.; Al-Dhabi, N.A. Gallic acid attenuates high-fat diet fed-streptozotocin-induced insulin resistance via partial agonism of PPARγ in experimental type 2 diabetic rats and enhances glucose uptake through translocation and activation of GLUT4 in PI3K/p-Akt signaling pathway. Eur. J. Pharmacol. 2014, 745, 201–216. [Google Scholar] [CrossRef] [PubMed]
  23. Hirai, T.; Chida, K. Protein kinase Cζ (PKCζ): Activation mechanisms and cellular functions. J. Biochem. 2003, 133, 1–7. [Google Scholar] [CrossRef] [PubMed]
  24. Prasad, C.N.V.; Anjana, T.; Banerji, A.; Gopalakrishnapillai, A. Gallic acid induces GLUT4 translocation and glucose uptake activity in 3T3-L1 cells. FEBS Lett. 2010, 584, 531–536. [Google Scholar] [CrossRef] [PubMed]
  25. Tzeng, T.F.; Liou, S.S.; Liu, I.M. Myricetin ameliorates defective post-receptor insulin signaling via β-endorphin signaling in the skeletal muscles of fructose-fed rats. Evid.-Based Complement. Altern. Med. 2011, 1–9. [Google Scholar] [CrossRef] [PubMed]
  26. Silva, D.D.; Zancan, P.; Coelho, W.S.; Gomez, L.S.; Sola-Penna, M. Metformin reverses hexokinase and 6-phosphofructo-1-kinase inhibition in skeletal muscle, liver and adipose tissue from STZ-induced diabetic mouse. Arch. Biochem. Biophys. 2010, 496, 53–60. [Google Scholar] [CrossRef] [PubMed]
  27. Vibhor, G.; Rameshwar, N.K.B. Human pyruvate kinase M2: A multifunctional protein. Protein Sci. 2010, 19, 2031–2044. [Google Scholar]
  28. Iori, E.; Millioni, R.; Puricelli, L.; Arrigoni, G.; Lenzini, L.; Trevisan, R.; James, P.; Rossi, G.P.; Pinna, L.A.; Tessari, P. Glycolytic enzyme expression and pyruvate kinase activity in cultured fibroblasts from type 1 diabetic patients with and without nephropathy. Biochim. Biophys. Acta Mol. Basis Dis. 2008, 1782, 627–633. [Google Scholar] [CrossRef] [PubMed]
  29. Parks, W.C.; Darke, R.L. Insulin mediates the stimulation of pyruvate kinase by a dual mechanism. Biochem. J. 1982, 208, 333–337. [Google Scholar] [CrossRef] [PubMed]
  30. Brasaemle, D.L. Lipolysis control: The plot thickens. Cell Metab. 2010, 11, 173–174. [Google Scholar] [CrossRef] [PubMed]
  31. Zimmermann, R.; Strauss, J.G.; Haemmerle, G.; Schoiswohl, G.; Birner-Gruenberger, R.; Riederer, M.; Lass, A.; Neuberger, G.; Eisenhaber, F.; Hermetter, A.; et al. Fat mobilization in adipose tissue is promoted by adipose triglyceride lipase. Science 2004, 306, 1383–1386. [Google Scholar] [CrossRef] [PubMed]
  32. Jocken, J.W.E.; Langin, D.; Smit, E.; Saris, W.H.M.; Valle, C.; Hul, G.B.; Holm, C.; Arner, P.; Blaak, E.E. Adipose triglyceride lipase and hormone-sensitive lipase protein expression is decreased in the obese insulin-resistant state. J. Clin. Endocrinol. Metab. 2007, 92, 2292–2299. [Google Scholar] [CrossRef] [PubMed]
  33. Morak, M.; Schmidinger, H.; Riesenhuber, G.; Rechberger, G.N.; Kollroser, M.; Haemmerle, G.; Zechner, R.; Kronenberg, F.; Hermetter, A. Adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) deficiencies affect expression of lipolytic activities in mouse adipose tissues. Mol. Cell. Proteom. 2012, 11, 1777–1789. [Google Scholar] [CrossRef] [PubMed]
  34. Rajalakshmi, K.; Devaraj, H.; Niranjali Devaraj, S. Assessment of the no-observed-adverse-effect level (NOAEL) of gallic acid in mice. Food Chem. Toxicol. 2001, 39, 919–922. [Google Scholar] [CrossRef]
  35. Niho, N.; Shibutani, M.; Tamura, T.; Toyoda, K.; Uneyama, C.; Takahashi, N. Subchronic toxicity study of gallic acid by oral administration in F344 rats. Food Chem. Toxicol. 2001, 39, 1063–1070. [Google Scholar] [CrossRef]
  36. U.S. Food and Drug Administration. Guidance for Industry and Reviewers: Estimating the Safe Starting Dose in Clinical Trials for Theaputics in Adult Healthy Volunteers. 2005. Available online: https://www.fda.gov/downloads/drugs/guidances/ucm078932.pdf (accessed on 14 January 2018).
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Figure 1. The perirenal and epididymal adipose tissues weight of HFD rats after being fed with GA for four weeks. GA: gallic acid; Normal: Normal rats were treated with saline; HFD: High fructose diet rats were treated with saline; HFD + Pio (30): High fructose diet rats treated with pioglitazone (30 mg/kg body weight); HFD + GA (30): High fructose diet rats treated with gallic acid (30 mg/kg body weight); HFD + GA (10): High fructose diet rats treated with gallic acid (10 mg/kg body weight). Values calculated as mean ± SD for six rats in each group. * p < 0.05, versus HFD rat for perirenal adipose. # p < 0.05, versus HFD rat for epididymal adipose tissue.
Figure 1. The perirenal and epididymal adipose tissues weight of HFD rats after being fed with GA for four weeks. GA: gallic acid; Normal: Normal rats were treated with saline; HFD: High fructose diet rats were treated with saline; HFD + Pio (30): High fructose diet rats treated with pioglitazone (30 mg/kg body weight); HFD + GA (30): High fructose diet rats treated with gallic acid (30 mg/kg body weight); HFD + GA (10): High fructose diet rats treated with gallic acid (10 mg/kg body weight). Values calculated as mean ± SD for six rats in each group. * p < 0.05, versus HFD rat for perirenal adipose. # p < 0.05, versus HFD rat for epididymal adipose tissue.
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Figure 2. Effect of GA on the expression of insulin signal transduction-related proteins (A) insulin receptor (IR) and GLUT4, and (B) PKC-ζ in adipose tissues of HFD rats. GA: gallic acid; IR: insulin receptor; GLUT4: glucose transporter 4; PKC-ζ: protein kinase C-zeta; Normal: rats fed a normal diet; HFD: rats fed a 66% fructose diet; HFD + Pio (30): rats fed a 66% fructose diet and orally administered pioglitazone (30 mg/kg body weight); HFD + GA (30): rats fed a 66% fructose diet and orally administered GA (30 mg/kg body weight); HFD + GA (10): rats fed a 66% fructose diet and orally administered GA (10 mg/kg body weight). Different letters (a–c) indicate a significant difference at p < 0.05. Values calculated as the means ± SD for six rats in each group. The relative expressions of IR, GLUT4, and PKC-ζ in each treatment group were calculated using α-tublin as the standard.
Figure 2. Effect of GA on the expression of insulin signal transduction-related proteins (A) insulin receptor (IR) and GLUT4, and (B) PKC-ζ in adipose tissues of HFD rats. GA: gallic acid; IR: insulin receptor; GLUT4: glucose transporter 4; PKC-ζ: protein kinase C-zeta; Normal: rats fed a normal diet; HFD: rats fed a 66% fructose diet; HFD + Pio (30): rats fed a 66% fructose diet and orally administered pioglitazone (30 mg/kg body weight); HFD + GA (30): rats fed a 66% fructose diet and orally administered GA (30 mg/kg body weight); HFD + GA (10): rats fed a 66% fructose diet and orally administered GA (10 mg/kg body weight). Different letters (a–c) indicate a significant difference at p < 0.05. Values calculated as the means ± SD for six rats in each group. The relative expressions of IR, GLUT4, and PKC-ζ in each treatment group were calculated using α-tublin as the standard.
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Figure 3. Effect of GA on the expression of carbohydrate (A) and lipid (B) metabolism-related proteins in adipose tissue of HFD rats. GA: gallic acid; PFK: phosphofructokinase; PK: pyruvate kinase; ATGL: adipose triglyceride lipase; Normal: rats fed a normal diet; HFD: rats fed a 66% fructose diet; HFD + Pio (30): rats fed a 66% fructose diet and orally administered pioglitazone (30 mg/kg body weight); HFD + GA (30): rats fed a 66% fructose diet and orally administered GA (30 mg/kg body weight); HFD + GA (10): rats fed a 66% fructose diet and orally administered GA (10 mg/kg body weight). Different letters (a–c) indicate a significant difference at p < 0.05. Values calculated as the means ± SD for six rats in each group. The relative expressions of PFK, PK, and ATGL in each treatment group were calculated using α-tublin as the standard.
Figure 3. Effect of GA on the expression of carbohydrate (A) and lipid (B) metabolism-related proteins in adipose tissue of HFD rats. GA: gallic acid; PFK: phosphofructokinase; PK: pyruvate kinase; ATGL: adipose triglyceride lipase; Normal: rats fed a normal diet; HFD: rats fed a 66% fructose diet; HFD + Pio (30): rats fed a 66% fructose diet and orally administered pioglitazone (30 mg/kg body weight); HFD + GA (30): rats fed a 66% fructose diet and orally administered GA (30 mg/kg body weight); HFD + GA (10): rats fed a 66% fructose diet and orally administered GA (10 mg/kg body weight). Different letters (a–c) indicate a significant difference at p < 0.05. Values calculated as the means ± SD for six rats in each group. The relative expressions of PFK, PK, and ATGL in each treatment group were calculated using α-tublin as the standard.
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Figure 4. The postulated mechanism for GA to regulate carbohydrate and lipid metabolism via restoring insulin signaling, enhancing glycolysis and lipolysis pathways in perirenal adipocytes of high fructose diet-induced diabetic rats. GA: gallic acid; FFA: free fatty acid; DG: diglyceride; ATGL: adipose triglyceride lipase; TG: triglyceride; PKC-ζ: protein kinase C-zeta; PK: pyruvate kinase; GLUT-4: glucose transporter 4; PFK: phosphofructokinase; F-1,6-BP: fructose 1,6 biphosphate.
Figure 4. The postulated mechanism for GA to regulate carbohydrate and lipid metabolism via restoring insulin signaling, enhancing glycolysis and lipolysis pathways in perirenal adipocytes of high fructose diet-induced diabetic rats. GA: gallic acid; FFA: free fatty acid; DG: diglyceride; ATGL: adipose triglyceride lipase; TG: triglyceride; PKC-ζ: protein kinase C-zeta; PK: pyruvate kinase; GLUT-4: glucose transporter 4; PFK: phosphofructokinase; F-1,6-BP: fructose 1,6 biphosphate.
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Huang, D.-W.; Chang, W.-C.; Yang, H.-J.; Wu, J.S.-B.; Shen, S.-C. Gallic Acid Alleviates Hypertriglyceridemia and Fat Accumulation via Modulating Glycolysis and Lipolysis Pathways in Perirenal Adipose Tissues of Rats Fed a High-Fructose Diet. Int. J. Mol. Sci. 2018, 19, 254. https://doi.org/10.3390/ijms19010254

AMA Style

Huang D-W, Chang W-C, Yang H-J, Wu JS-B, Shen S-C. Gallic Acid Alleviates Hypertriglyceridemia and Fat Accumulation via Modulating Glycolysis and Lipolysis Pathways in Perirenal Adipose Tissues of Rats Fed a High-Fructose Diet. International Journal of Molecular Sciences. 2018; 19(1):254. https://doi.org/10.3390/ijms19010254

Chicago/Turabian Style

Huang, Da-Wei, Wen-Chang Chang, Heng-Jui Yang, James Swi-Bea Wu, and Szu-Chuan Shen. 2018. "Gallic Acid Alleviates Hypertriglyceridemia and Fat Accumulation via Modulating Glycolysis and Lipolysis Pathways in Perirenal Adipose Tissues of Rats Fed a High-Fructose Diet" International Journal of Molecular Sciences 19, no. 1: 254. https://doi.org/10.3390/ijms19010254

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