lncRNAs in Non-Malignant Tissue Have Prognostic Value in Colorectal Cancer
Abstract
:1. Introduction
2. Results
2.1. Patient´s Characteristics
2.2. Expression Fold Change of lncRNAs (long non-coding RNAs) in Tumour Tissue (TT) Compared to Non-Malignant Tissue (MT)
2.3. Expression of lncRNAs in Non-malignant and Tumour Tissue
2.4. Expression Ratios of Two lncRNAs in Non-Malignant and Tumour Tissues
3. Discussion
3.1. Differential Expression Values in TT Compared to MT
3.2. Normalized Expression in TT and MT
3.3. lncRNA Expression Ratios
4. Materials and Methods
4.1. Patient Selection
4.2. RNA Extraction
4.3. RT-PCR
4.4. Quantitative-PCR (qPCR)
4.5. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
AJCC | American joint committee on cancer |
ANRIL | CDKN2B antisense RNA 1 |
CCAT1 | Colon cancer associated transcript 1 |
CCND | Cyclin D |
CRC | Colorectal cancer |
Ct | Cycle threshold |
DFS | Disease free survival |
EMT | Epithelial to mesenchymal transition |
GAS5 | Growth arrest-specific 5 |
HR | Hazard rate |
Linc-ROR | Long intergenic non-protein coding regulator of reprogramming |
lncRNA | Long non-coding RNA |
MALAT1 | Metastasis associated lung adenocarcinoma transcript 1 |
MIR155HG | MIR155 host gene |
MMR | DNA mismatch repair |
MT | Non-malignant (mucosa) tissue |
OS | Overall survival |
PCAT1 | Prostate cancer associated transcript 1 |
q-PCR | Quantitative PCR |
RT-PCR | Reverse transcription PCR |
SPRY4-IT1 | Sprouty homolog 4 intronic transcript 1 |
TP53 | Tumour protein p53 |
TT | Tumour tissue |
TUG1 | Taurine up-regulated 1 |
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Characteristic | Category | Number | % |
---|---|---|---|
gender | F | 24 | 38.1 |
M | 39 | 61.9 | |
age (in years) | 30–50 | 8 | 12.7 |
50–70 | 32 | 50.8 | |
>70 | 23 | 36.5 | |
T stage | T1 | 1 | 1.6 |
T2 | 14 | 22.2 | |
T3 | 40 | 63.5 | |
T4 | 6 | 9.5 | |
unknown | 2 | 3.2 | |
N stage | N0 | 39 | 61.9 |
N1 | 13 | 20.6 | |
N2 | 10 | 15.9 | |
unknown | 1 | 1.6 | |
M stage | 0 | 47 | 74.6 |
1 | 11 | 17.5 | |
unknown | 5 | 7.9 | |
tumour grade | G1 | 12 | 19.0 |
G2 | 42 | 66.7 | |
G3 | 5 | 7.9 | |
unknown | 4 | 6.3 | |
AJCC staging | I | 11 | 17.5 |
II | 19 | 30.2 | |
III | 14 | 22.2 | |
IV | 11 | 17.5 | |
unknown | 8 | 12.7 | |
primary tumour location | right or transversum | 16 | 25.4 |
Left or sigma | 10 | 15.9 | |
rectum or rectosigma | 31 | 49.2 | |
non-specific | 6 | 9.5 |
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Thiele, J.-A.; Hosek, P.; Kralovcova, E.; Ostasov, P.; Liska, V.; Bruha, J.; Vycital, O.; Rosendorf, J.; Opattova, A.; Horak, J.; et al. lncRNAs in Non-Malignant Tissue Have Prognostic Value in Colorectal Cancer. Int. J. Mol. Sci. 2018, 19, 2672. https://doi.org/10.3390/ijms19092672
Thiele J-A, Hosek P, Kralovcova E, Ostasov P, Liska V, Bruha J, Vycital O, Rosendorf J, Opattova A, Horak J, et al. lncRNAs in Non-Malignant Tissue Have Prognostic Value in Colorectal Cancer. International Journal of Molecular Sciences. 2018; 19(9):2672. https://doi.org/10.3390/ijms19092672
Chicago/Turabian StyleThiele, Jana-Aletta, Petr Hosek, Eva Kralovcova, Pavel Ostasov, Vaclav Liska, Jan Bruha, Ondrej Vycital, Jachym Rosendorf, Alena Opattova, Josef Horak, and et al. 2018. "lncRNAs in Non-Malignant Tissue Have Prognostic Value in Colorectal Cancer" International Journal of Molecular Sciences 19, no. 9: 2672. https://doi.org/10.3390/ijms19092672
APA StyleThiele, J. -A., Hosek, P., Kralovcova, E., Ostasov, P., Liska, V., Bruha, J., Vycital, O., Rosendorf, J., Opattova, A., Horak, J., Kralickova, M., Vodicka, P., & Pitule, P. (2018). lncRNAs in Non-Malignant Tissue Have Prognostic Value in Colorectal Cancer. International Journal of Molecular Sciences, 19(9), 2672. https://doi.org/10.3390/ijms19092672