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Volume 20
Issue 11
10.3390/ijms20112657
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Review
Peer-Review Record

DNA-Methylation-Based Detection of Urological Cancer in Urine: Overview of Biomarkers and Considerations on Biomarker Design, Source of DNA, and Detection Technologies

Int. J. Mol. Sci. 2019, 20(11), 2657; https://doi.org/10.3390/ijms20112657
by Louise Katrine Larsen 1, Guro Elisabeth Lind 2, Per Guldberg 1 and Christina Dahl 1,*
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Int. J. Mol. Sci. 2019, 20(11), 2657; https://doi.org/10.3390/ijms20112657
Submission received: 29 April 2019 / Revised: 23 May 2019 / Accepted: 24 May 2019 / Published: 30 May 2019
(This article belongs to the Special Issue Epigenetics of Urological Cancers)

Round  1

Reviewer 1 Report

Authors reviewed the role of urinary DNA methylation biomarker for urological cancer, showing their potential impact in the detection of bladder, prostate, renal cancer. Authors explored the literature available using strictly inclusion criteria, as further specified in Supplementary Material. Use of sources was appropriate and statistical analysis was well performed in order to avoid evaluation biases.

It is an interesting topic, and the review performs a good analysis of the current knowledge in the field of urine-based DNA tests, giving a significant contribution for the readers. Figures and tables illustrating the data have been well built. The article is well written, logically structured and fluent to read, notwithstanding the not easy topic. I suggest to accept the paper in the present form.

Author Response

We thank the reviewer for carefully reading our manuscript and appreciate the positive response.

Reviewer 2 Report

This is well written review of DNA methylation-based detection of urologic cancer in the urine. The authors described different outcomes of DNA methylation in prostate, bladder and renal cell carcinoma. I have no major comments and only minor comments are the tables included may be simplified and include a legend. In addition, in the summary it would be good if the authors gave some direction where this field should go in the future and also compare\contrast if any of these DNA methylation based detection assays have been helpful in other malignancies. Can we learn from other diseases?

Author Response

We thank the reviewer for carefully reading our manuscript and appreciate the positive response.

 We have carefully gone over all the material included in the tables and provided appropriate legends.

 We have included a sentence in the Abstract emphasizing the need for prospective trials and careful selection of control groups in order to move the field forward.

 We feel that the manuscript is already quite long and complex as it is, covering several different cancer forms. Expanding the discussion with additional cancer forms would add another level of complexity that we would like to avoid, not least because it would imply a comparison between plasma and urine as a substrate for liquid biopsy.


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