Endometrial Stromal Cells Circulate in the Bloodstream of Women with Endometriosis: A Pilot Study
Abstract
:1. Introduction
2. Results
Isolation and Detection of Circulating Endometrial Cells (CECs): Immunofluorescence
3. Discussion
4. Material and Methods
4.1. Subjects
4.2. Sample Collection
4.3. Sample Processing
4.4. Detection of CECs: Immunofluorescence
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
CD10 | Common acute lymphoblastic leukemia-associated |
CECs | Circulating Endometrial Cells |
CK | Cytokeratin |
COS | Controlled Ovarian Stimulation |
CRCs | Circulating Rare Cells |
CTCs | Circulating Tumor Cells |
DIE | Deep Infiltrating Endometriosis |
EMT | Epithelial Mesenchymal Transition |
EpCAM | Epithelial Cell Adhesion Molecule |
FDA | Food and Drug Administration |
GnRH | Gonadotropin Releasing Hormone |
IHC | Immunohistochemistry |
IF | Immunofluorescence |
LGR5 | Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 |
rFSH | Recombinant follicular stimulating hormone |
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Patient Code | Age | Menstrual Cycle Phase | Type of Endometriosis | Stimulation | CECs | |
---|---|---|---|---|---|---|
CK | CD10 | |||||
1 | 26 | Proliferative | Control | rFSH (antagonist) | 0 | 0 |
2 | 31 | Proliferative | Control | rFSH (antagonist) | 0 | 0 |
3 | 21 | Proliferative | Control | rFSH (antagonist) | 0 | 0 |
4 | 22 | Proliferative | Control | rFSH (antagonist) | 0 | 0 |
5 | 37 | Proliferative | Endometrioma | rFSH (antagonist) | 0 | 11 |
6 | 36 | Proliferative | DIE | rFSH (antagonist) | 0 | 5 |
7 | 42 | Secretory | Endometrioma | HRT | 0 | 17 |
8 | 32 | Proliferative | DIE | GnRH downregulation + HRT | 0 | 0 |
9 | 30 | Menstrual | DIE | HRT | 0 | 3 |
9 | 30 | Proliferative | DIE | HRT | 0 | 57 |
9 | 30 | Secretory | DIE | HRT | 0 | 22 |
9 | 30 | Proliferative | DIE | GnRH downregulation + HRT | 0 | 3 |
Gynecological Pathology | Benign/Malignant | CTCs/CECs Detection Technology | References |
---|---|---|---|
Endometrial cancer | Malignant | EpCAM based; CellSearch® and IF | [41] |
EpCAM based; CellSearch® and IF | [46] | ||
Density-based, Enrichment (Oncoquick) and RTqPCR | [44] | ||
RTqPCR and flow cytometry | [43] | ||
Size-Based Enrichment (Metacell®) and Immunodetection | [53] | ||
RTqPCR | [45] | ||
Review | [49] | ||
Breast cancer | Malignant | EpCAM based; CellSearch® | [27] |
EpCAM based; CellSearch® | [29] | ||
EpCAM based; CellSearch® | [28] | ||
Ovarian cancer | Malignant | EpCAM based; magnetic beads | [50] |
ICC | [51] | ||
Immuomagnetic bead screening and RTqPCR | [47] | ||
Review | [52] | ||
Immunomagnetic microspheres | [42] | ||
Microfluidic system | [48] | ||
Endometriosis | Benign | In culture enrichment; MetaCell® | [39] |
IF staining via microfluidic chips | [40] | ||
ScreenCell® | This study |
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Vallvé-Juanico, J.; López-Gil, C.; Ballesteros, A.; Santamaria, X. Endometrial Stromal Cells Circulate in the Bloodstream of Women with Endometriosis: A Pilot Study. Int. J. Mol. Sci. 2019, 20, 3740. https://doi.org/10.3390/ijms20153740
Vallvé-Juanico J, López-Gil C, Ballesteros A, Santamaria X. Endometrial Stromal Cells Circulate in the Bloodstream of Women with Endometriosis: A Pilot Study. International Journal of Molecular Sciences. 2019; 20(15):3740. https://doi.org/10.3390/ijms20153740
Chicago/Turabian StyleVallvé-Juanico, Júlia, Carlos López-Gil, Agustín Ballesteros, and Xavier Santamaria. 2019. "Endometrial Stromal Cells Circulate in the Bloodstream of Women with Endometriosis: A Pilot Study" International Journal of Molecular Sciences 20, no. 15: 3740. https://doi.org/10.3390/ijms20153740