CRB2 Loss in Rod Photoreceptors Is Associated with Progressive Loss of Retinal Contrast Sensitivity
Abstract
:1. Introduction
2. Results
2.1. Rho-iCre Mediates Recombination Specifically in Rod Photoreceptors
2.2. Ablation of CRB2 in Rods with Concomitant Loss of CRB1 Leads to Retinal Dysfunction and Vision Impairment
2.3. Pupil Light Reflex is not Impaired in Crb1KOCrb2ΔRods Mice
2.4. Loss of CRB2 in Rods Results in Slow Loss of Photoreceptor Cells, Mainly in the Superior Retina
2.5. Removal of CRB2 in Rods Results in Loss of Rods Mainly at the Periphery of the Superior Retina
2.6. Loss of CRB2 in Rods Leads to Disruption of the Apical Protein Complexes
2.7. Removal of CRB2 from Rods Results in Gliosis in Müller Glial Cells
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Chromosomal DNA Isolation and Genotyping
4.3. Electroretinography (ERG)
4.4. Pupillary Light Reflex
4.5. Optokinetic Tracking Reflex (OKT)
4.6. Morphological and Immunohistochemical Analysis
4.7. Transmission Electron Microscopy
4.8. Primary Antibodies
4.9. Quantification of the Number of Photoreceptor Nuclei in a Row
4.10. Statistical Analysis
5. Conclusions
6. Patents
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
CAR | Cone arrestin |
CRB1 | Crumbs homolog-1 |
ERG | Electroretinography |
GFAP | Glial fibrillary acidic protein |
GS | Glutamine synthetase |
LCA | Leber congenital amaurosis |
MGC | Müller glial cell |
OKT | Optokinetic tracking reflex |
PLR | Pupillary light reflex |
PNA | Peanut agglutinin |
RP | Retinitis pigmentosa |
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Alves, C.H.; Boon, N.; Mulder, A.A.; Koster, A.J.; Jost, C.R.; Wijnholds, J. CRB2 Loss in Rod Photoreceptors Is Associated with Progressive Loss of Retinal Contrast Sensitivity. Int. J. Mol. Sci. 2019, 20, 4069. https://doi.org/10.3390/ijms20174069
Alves CH, Boon N, Mulder AA, Koster AJ, Jost CR, Wijnholds J. CRB2 Loss in Rod Photoreceptors Is Associated with Progressive Loss of Retinal Contrast Sensitivity. International Journal of Molecular Sciences. 2019; 20(17):4069. https://doi.org/10.3390/ijms20174069
Chicago/Turabian StyleAlves, C. Henrique, Nanda Boon, Aat A. Mulder, Abraham J. Koster, Carolina R. Jost, and Jan Wijnholds. 2019. "CRB2 Loss in Rod Photoreceptors Is Associated with Progressive Loss of Retinal Contrast Sensitivity" International Journal of Molecular Sciences 20, no. 17: 4069. https://doi.org/10.3390/ijms20174069
APA StyleAlves, C. H., Boon, N., Mulder, A. A., Koster, A. J., Jost, C. R., & Wijnholds, J. (2019). CRB2 Loss in Rod Photoreceptors Is Associated with Progressive Loss of Retinal Contrast Sensitivity. International Journal of Molecular Sciences, 20(17), 4069. https://doi.org/10.3390/ijms20174069