The Methylation Patterns and Transcriptional Responses to Chilling Stress at the Seedling Stage in Rice
Abstract
:1. Introduction
2. Results
2.1. The Effects of Low-Temperature Stress on Seed Germination, Growth, and Survival of Three Rice Cultivars Seedlings
2.2. Physiological Response of Seedlings to Low Temperature
2.3. Comparison of DNA Methylation Levels and Loci
2.4. Functional Enrichment Analysis of the Methylation Differential Gene of P427
2.5. Comparison of mRNA Expression Differences Under Low-Temperature Stress
2.6. Combined Analysis of The Methylation and Expression Profiles of Unique Genes in P427
2.7. Validation of Gene Expression Profiles by qRT-PCR
3. Discussion
3.1. The ICE-CBF-COR Conduction Pathway Plays a Leading Role in The Cold Tolerance of Varieties
3.2. Association Analysis between DNA Methylation and Gene Expression
3.3. Excavating New Gene-Rich Cold-Tolerant Regulatory Pathways
4. Materials and Methods
4.1. Low-Temperature Resistance Screening of P427
4.2. Planting and Low-Temperature Treatment
4.3. Chlorophyll Content Determination
4.4. Hormone and Enzyme Content Determination
4.5. RNA-Seq Assay
4.6. MeDIP (Methylated DNA Immunoprecipitation Sequencing) Analysis
4.7. Real-Time Quantitative PCR Validation of The DEGs
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Guo, H.; Wu, T.; Li, S.; He, Q.; Yang, Z.; Zhang, W.; Gan, Y.; Sun, P.; Xiang, G.; Zhang, H.; et al. The Methylation Patterns and Transcriptional Responses to Chilling Stress at the Seedling Stage in Rice. Int. J. Mol. Sci. 2019, 20, 5089. https://doi.org/10.3390/ijms20205089
Guo H, Wu T, Li S, He Q, Yang Z, Zhang W, Gan Y, Sun P, Xiang G, Zhang H, et al. The Methylation Patterns and Transcriptional Responses to Chilling Stress at the Seedling Stage in Rice. International Journal of Molecular Sciences. 2019; 20(20):5089. https://doi.org/10.3390/ijms20205089
Chicago/Turabian StyleGuo, Hui, Tingkai Wu, Shuxing Li, Qiang He, Zhanlie Yang, Wuhan Zhang, Yu Gan, Pingyong Sun, Guanlun Xiang, Hongyu Zhang, and et al. 2019. "The Methylation Patterns and Transcriptional Responses to Chilling Stress at the Seedling Stage in Rice" International Journal of Molecular Sciences 20, no. 20: 5089. https://doi.org/10.3390/ijms20205089