Low Intensity Pulsed Ultrasound Prevents Recurrent Ischemic Stroke in a Cerebral Ischemia/Reperfusion Injury Mouse Model via Brain-derived Neurotrophic Factor Induction
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Animals and Middle Cerebral Artery Occlusion (MCAO) Recurrent Surgical Procedure
4.2. LIPUS Treatment
4.3. Neurological Function Assessment
4.4. Locomotor Activity Evaluation
4.5. Assessment of Motor Equilibrium Performance on Rotarod
4.6. Infarct Volume Detection
4.7. Histopathological and Immunohistochemical Analysis
4.8. Western Blotting
4.9. Fluorescent Terminal Deoxynucleotidyl Transferase (TdT) dUTP Nick End Labeling (TUNEL) Staining
4.10. Statistics
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
Abbreviations
LIPUS | Low intensity pulsed ultrasound |
BDNF | Brain-derived neurotrophic factor |
MCAO | Middle cerebral artery occlusion |
TUNEL | Terminal deoxynucleotidyl transferase (TdT) dUTP nick end labeling |
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Wu, C.-T.; Yang, T.-H.; Chen, M.-C.; Chung, Y.-P.; Guan, S.-S.; Long, L.-H.; Liu, S.-H.; Chen, C.-M. Low Intensity Pulsed Ultrasound Prevents Recurrent Ischemic Stroke in a Cerebral Ischemia/Reperfusion Injury Mouse Model via Brain-derived Neurotrophic Factor Induction. Int. J. Mol. Sci. 2019, 20, 5169. https://doi.org/10.3390/ijms20205169
Wu C-T, Yang T-H, Chen M-C, Chung Y-P, Guan S-S, Long L-H, Liu S-H, Chen C-M. Low Intensity Pulsed Ultrasound Prevents Recurrent Ischemic Stroke in a Cerebral Ischemia/Reperfusion Injury Mouse Model via Brain-derived Neurotrophic Factor Induction. International Journal of Molecular Sciences. 2019; 20(20):5169. https://doi.org/10.3390/ijms20205169
Chicago/Turabian StyleWu, Cheng-Tien, Ting-Hua Yang, Man-Chih Chen, Yao-Pang Chung, Siao-Syun Guan, Lin-Hwa Long, Shing-Hwa Liu, and Chang-Mu Chen. 2019. "Low Intensity Pulsed Ultrasound Prevents Recurrent Ischemic Stroke in a Cerebral Ischemia/Reperfusion Injury Mouse Model via Brain-derived Neurotrophic Factor Induction" International Journal of Molecular Sciences 20, no. 20: 5169. https://doi.org/10.3390/ijms20205169