ATG 4B Serves a Crucial Role in RCE-4-Induced Inhibition of the Bcl-2–Beclin 1 Complex in Cervical Cancer Ca Ski Cells
Abstract
:1. Introduction
2. Results
2.1. RCE-4 Induces Time- and Concentration-Dependent Apoptosis and Autophagy in Ca Ski Cells
2.2. RCE-4 Inhibits the Formation of Bcl-2–Beclin 1 Complex in Ca Ski Cells
2.3. RCE-4 Inhibits the Formation of Bcl-2–Beclin 1 Complex via Various Pathways
2.4. ATG 4B Plays a Critical Role in the Inhibition of Bcl-2–Beclin 1 Complex Induced by RCE-4
2.5. RCE-4 Combined with ATG siRNA Enhances the Sensitivity to Ca Ski Cells
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Reagent and Antibodies
4.3. Cytotoxicity Assay
4.4. Flow Cytometry Assay
4.5. Acridine Orange/Ethidium Bromide (AO/EB) Double Fluorescent Staining
4.6. MMP Assay
4.7. Western Blotting
4.8. Plasmid Small Interfering (si)RNA Transfection
4.9. Co-Immunoprecipitation (Co-IP)
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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You, F.-F.; Zhang, J.; Cheng, F.; Zou, K.; Zhang, X.-Q.; Chen, J.-F. ATG 4B Serves a Crucial Role in RCE-4-Induced Inhibition of the Bcl-2–Beclin 1 Complex in Cervical Cancer Ca Ski Cells. Int. J. Mol. Sci. 2021, 22, 12302. https://doi.org/10.3390/ijms222212302
You F-F, Zhang J, Cheng F, Zou K, Zhang X-Q, Chen J-F. ATG 4B Serves a Crucial Role in RCE-4-Induced Inhibition of the Bcl-2–Beclin 1 Complex in Cervical Cancer Ca Ski Cells. International Journal of Molecular Sciences. 2021; 22(22):12302. https://doi.org/10.3390/ijms222212302
Chicago/Turabian StyleYou, Fang-Fang, Jing Zhang, Fan Cheng, Kun Zou, Xue-Qing Zhang, and Jian-Feng Chen. 2021. "ATG 4B Serves a Crucial Role in RCE-4-Induced Inhibition of the Bcl-2–Beclin 1 Complex in Cervical Cancer Ca Ski Cells" International Journal of Molecular Sciences 22, no. 22: 12302. https://doi.org/10.3390/ijms222212302