Covalently Immobilized Regenerable Immunoaffinity Layer with Orientation-Controlled Antibodies Based on Z-Domain Autodisplay
Abstract
:1. Introduction
2. Results and Discussion
2.1. Optimization of Chemical Immobilizing Conditions
2.2. Regenerable Immunoassay Based on Covalently Immobilized Antibodies with Orientation Control
2.3. Application of Covalently Immobilized Regenerable Immunoaffinity Layer with Orientation Control in an SPR Biosensor
3. Materials and Methods
3.1. Reagent
3.2. Cultivation of Autodisplaying E. coli Cells and OM Isolation
3.3. Regenerable Test of Covalently Immobilized Immunoaffinity Layer with Orientation Control
3.4. SPR Biosensor Measurement
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Park, J.-M.; Kim, M.Y.; Jose, J.; Park, M. Covalently Immobilized Regenerable Immunoaffinity Layer with Orientation-Controlled Antibodies Based on Z-Domain Autodisplay. Int. J. Mol. Sci. 2022, 23, 459. https://doi.org/10.3390/ijms23010459
Park J-M, Kim MY, Jose J, Park M. Covalently Immobilized Regenerable Immunoaffinity Layer with Orientation-Controlled Antibodies Based on Z-Domain Autodisplay. International Journal of Molecular Sciences. 2022; 23(1):459. https://doi.org/10.3390/ijms23010459
Chicago/Turabian StylePark, Jong-Min, Mi Yeon Kim, Joachim Jose, and Min Park. 2022. "Covalently Immobilized Regenerable Immunoaffinity Layer with Orientation-Controlled Antibodies Based on Z-Domain Autodisplay" International Journal of Molecular Sciences 23, no. 1: 459. https://doi.org/10.3390/ijms23010459