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Article
Peer-Review Record

Karyotype Analysis, Genomic and Fluorescence In Situ Hybridization (GISH and FISH) Reveal the Ploidy and Parental Origin of Chromosomes in Paeonia Itoh Hybrids

Int. J. Mol. Sci. 2022, 23(19), 11406; https://doi.org/10.3390/ijms231911406
by Litao Cui, Tai Chen, Xin Zhao, Shunli Wang, Xiuxia Ren, Jingqi Xue * and Xiuxin Zhang *
Reviewer 1: Anonymous
Int. J. Mol. Sci. 2022, 23(19), 11406; https://doi.org/10.3390/ijms231911406
Submission received: 8 September 2022 / Revised: 22 September 2022 / Accepted: 26 September 2022 / Published: 27 September 2022
(This article belongs to the Collection Feature Papers in Molecular Genetics and Genomics)

Round 1

Reviewer 1 Report

Thanks for submitting an article to this journal.
You must revise the entire article.
Correct spelling mistakes.
Correct grammar mistakes.
About Karyotype analysis, Reinforce the introduction and discussion sections and refer to other related references such as:

Farshadfar M. Chromosome location of ISSR markers and genes controlling seed germination under drought stress in wheat-barley disomic addition lines. Cell Mol Biol (Noisy-le-grand). 2020 Apr 20;66(1):101-108. PMID: 32359392.

Ebrahimzadeh-Vesal R, Shokrgozar MA, Nayernia K, Teimoori-Toolabi L, Estiar MA, Miryounesi M, Nourashrafeddin S, Modarressi MH. MicroRNA profiling during germline differentiation of mouse embryonic stem cells. Cell Mol Biol (Noisy-le-grand). 2015 Jul 31;61(3):84-91. PMID: 26255136.

Huang X, Ju H, Song G. Evaluation of basal hormone levels and androgen receptor gene mutations in individuals with recurrent abortion. Cell Mol Biol (Noisy-le-grand). 2022 Jan 2;67(4):274-281. doi: 10.14715/cmb/2021.67.4.30. PMID: 35809279.

Author Response

Thank you very much for your kindly help and good suggestions. We are very appreciated for it. We carefully revised our manuscript and corrected some spelling and grammar mistakes as well as giving more discussion on karyotype analysis. We also added all the references you recommend. Please find more details in our new submitted file. If you have any other suggestions or ideas, please feel free to let us know. We’d like to revise it again to make our work more meaningful.

Reviewer 2 Report

Dear Authors,

The article has tremendous application in the field of plant hybridization, where they can see the segregation pattern in F1 progenies where genome sequence data is not available.

Regards

K. N. Chandrashekara

Author Response

Dear Prof. Chandrashekara,

Thank you very much for your hard work and kindly help, and we are very appreciated for your recognition of our work. We carefully revised our manuscript again and corrected some spelling and grammar mistakes. Please find more details in our new submitted file. If you have any other suggestions or ideas, please let us know. We’d like to revise it again to make our work more meaningful.

Best wishes,

Xiuxin Zhang and all the authors

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