Effect of LDHA Inhibition on TNF-α-Induced Cell Migration in Esophageal Cancers
Abstract
:1. Introduction
2. Results
2.1. LDHA Is Upregulated in Esophageal Cancer Tissues
2.2. TNF-α Induces an Increase in LDHA and LDHB Gene Expression
2.3. Sodium Oxamate (SO) Decreases Secretion of Lactic Acid and Inhibits TNF-α-Induced Cell Migration
2.4. Oxamate Inhibits TNF-α-Induced MMP9 Expression by Affecting ERK1/2 Signal Transduction
2.5. Lactate Acid Enhances TNF-α-Induced Cell Migration and MMP9 Expression in Esophageal Cancer Cells
3. Materials and Methods
3.1. Analysis of LDHA Gene Expression in Esophageal Cancer Tissues
3.2. Cell Culture and Treatment
3.3. Zymography
3.4. Immunohistochemistry (IHC)
3.5. Western Blotting
3.6. Quantitative Polymerase Chain Reaction (qPCR)
3.7. Wound Healing Assay
3.8. Statistical Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Forkasiewicz, A.; Stach, W.; Wierzbicki, J.; Stach, K.; Tabola, R.; Hryniewicz-Jankowska, A.; Augoff, K. Effect of LDHA Inhibition on TNF-α-Induced Cell Migration in Esophageal Cancers. Int. J. Mol. Sci. 2022, 23, 16062. https://doi.org/10.3390/ijms232416062
Forkasiewicz A, Stach W, Wierzbicki J, Stach K, Tabola R, Hryniewicz-Jankowska A, Augoff K. Effect of LDHA Inhibition on TNF-α-Induced Cell Migration in Esophageal Cancers. International Journal of Molecular Sciences. 2022; 23(24):16062. https://doi.org/10.3390/ijms232416062
Chicago/Turabian StyleForkasiewicz, Agata, Wojciech Stach, Jaroslaw Wierzbicki, Kamilla Stach, Renata Tabola, Anita Hryniewicz-Jankowska, and Katarzyna Augoff. 2022. "Effect of LDHA Inhibition on TNF-α-Induced Cell Migration in Esophageal Cancers" International Journal of Molecular Sciences 23, no. 24: 16062. https://doi.org/10.3390/ijms232416062