Ru(II) Oxygen Sensors for Co(III) Complexes and Amphotericin B Antifungal Activity Detection by Phosphorescence Optical Respirometry
Abstract
:1. Introduction
2. Results and Discussion
2.1. Structural Characterization of the Coordination Compounds Studied
2.2. Monitoring Microbial Growth Using Phosphorescence Optical Respirometry
2.3. Study of the Effect of Co(III) Coordination Compounds and Amphotericin B on Yeast by Phosphorescence Optical Respirometry
2.3.1. Measurements Using Box, BsOx in RPMI Medium
2.3.2. Measurements Using Box and BsOx in 5% and 50% Bovine Serum Albumin (BSA)
3. Materials and Methods
3.1. Strains, Media, and Growth Conditions
3.2. Chemicals, Methodology, and Apparatus
3.3. The Synthesis of the Tris-[(4,7-Diphenyl-1,10-Phenanthrolinedisulphonic Acid Disodium) Ruthenium(II)] Chloride Hydrate (BsOx)
3.4. The Synthesis of the Tris-[(4,7-Diphenyl-1,10-Phenanthroline) Ruthenium(II)] Chloride Pentahydrate (Box)
3.5. The Synthesis of Compounds (1) and (2)
3.6. Study of the Effect of Co(III) Coordination Compounds and Amphotericin B on Yeast by Phosphorescence Optical Respirometry
3.6.1. Measurements Using Box and BsOx in RPMI Medium
3.6.2. Measurements Using Box and BsOx in 5% and 50% Bovine Serum Albumin (BSA)
3.7. Determination of the Minimum Inhibitory Concentration (MIC) in RPMI Medium and Bovine Serum Albumin
3.7.1. Determination of the Minimum Inhibitory Concentration in the RPMI Medium
3.7.2. Determination of the Minimum Inhibitory Concentration in Bovine Serum Albumin
3.8. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Strains | MIC of (1) | MIC of (2) | MIC of AmB |
---|---|---|---|
C. albicans ATCC 10231 | 62.5 ± 4.93 | 62.5 ± 4.01 | 0.5 ± 0.06 |
C. albicans 12823 | 62.5 ± 4.01 | 62.5 ± 4.92 | 0.5 ± 0.06 |
Strains | MIC of (1) | MIC of (2) | MIC of AmB | |||
---|---|---|---|---|---|---|
5% BSA | 50% BSA | 5% BSA | 50% BSA | 5% BSA | 50% BSA | |
C. albicans ATCC 10231 | 500 ± 112 | 1000 ± 224 | >1000 | >1000 | 0.25 ± 0.06 | 0.25 ± 0.07 |
C. albicans 12823 | 500 ± 137 | 1000 ± 274 | >1000 | >1000 | 0.25 ± 0.07 | 0.25 ± 0.07 |
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Turecka, K.; Chylewska, A.; Dąbrowska, A.M.; Hałasa, R.; Orlewska, C.; Waleron, K. Ru(II) Oxygen Sensors for Co(III) Complexes and Amphotericin B Antifungal Activity Detection by Phosphorescence Optical Respirometry. Int. J. Mol. Sci. 2023, 24, 8744. https://doi.org/10.3390/ijms24108744
Turecka K, Chylewska A, Dąbrowska AM, Hałasa R, Orlewska C, Waleron K. Ru(II) Oxygen Sensors for Co(III) Complexes and Amphotericin B Antifungal Activity Detection by Phosphorescence Optical Respirometry. International Journal of Molecular Sciences. 2023; 24(10):8744. https://doi.org/10.3390/ijms24108744
Chicago/Turabian StyleTurecka, Katarzyna, Agnieszka Chylewska, Aleksandra M. Dąbrowska, Rafał Hałasa, Czesława Orlewska, and Krzysztof Waleron. 2023. "Ru(II) Oxygen Sensors for Co(III) Complexes and Amphotericin B Antifungal Activity Detection by Phosphorescence Optical Respirometry" International Journal of Molecular Sciences 24, no. 10: 8744. https://doi.org/10.3390/ijms24108744