Revealing the Roles of the JAZ Family in Defense Signaling and the Agarwood Formation Process in Aquilaria sinensis
Abstract
:1. Introduction
2. Results
2.1. Phylogenetic Relationships and Major Domains of A. sinensis TIFY Family Members
2.2. Locations and Structures of the AsTIFY Genes and Promoter Elements of the AsJAZ Genes
2.3. Expression Patterns of the AsJAZ Genes
2.4. Chromosome Location of AsWRKY Genes and Phylogenetic Analysis of AsWRKY Proteins
2.5. Expression Patterns of AsWRKY Genes
2.6. Experimental Validation of the Interaction between AsJAZ4 and AsWRKY75n
3. Discussion
3.1. Classification of the AsJAZ Family Members
3.2. Expression Patterns of the AsJAZ Family Members
3.3. Classification and Expression Patterns of the AsWRKY Genes
3.4. Possible Roles of the AsJAZ4/WRKY75n Complex
4. Materials and Methods
4.1. Bioinformatic Analysis of TIFY Family Members in A. sinensis
4.2. Bioinformatic Analysis of WRKY Family Members
4.3. Sequence Alignment and Phylogenetic Analysis
4.4. Plant Sample Preparation
4.5. Real-Time Quantitative PCR
4.6. Statistical Analysis
4.7. Transcriptomic Analysis and Heatmap Illustrating
4.8. Protein Expression and Pull-Down Assay
4.9. Yeast Two-Hybrid
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Name | Synonym | a Chr. | b CDS (bp) | Protein | f ID | ||
---|---|---|---|---|---|---|---|
c Length (aa.) | d pI | e MW (Da) | |||||
AsJAZ1 | AsTIFY6a | 8 (−) | 1161 | 386 | 9.39 | 40,533.48 | Scaffold 331.1 |
AsJAZ2 | AsTIFY7a | 4 (−) | 1104 | 367 | 8.92 | 38,335.54 | Scaffold 5.283 |
AsJAZ3 | AsTIFY7b | 7 (−) | 903 | 300 | 9.28 | 31,788.02 | Scaffold 134.44 |
AsJAZ4 | AsTIFY5a | 3 (−) | 375 | 124 | 9.75 | 13,913.83 | Scaffold 262.13 g |
AsJAZ5 | AsTIFY5b | 3 (+) | 408 | 135 | 10.01 | 15,241.81 | Scaffold 166.1 h |
AsJAZ6 | AsTIFY10a | 8 (−) | 717 | 238 | 9.58 | 25,777.5 | Scaffold 46.130 |
AsJAZ7 | AsTIFY10b | 4 (+) | 777 | 258 | 9.02 | 27,571.31 | Scaffold 43.66 |
AsJAZ8 | AsTIFY10c | 4 (−) | 777 | 258 | 8.82 | 27,518.26 | Scaffold 5.466 |
AsJAZ9 | AsTIFY11a | 6 (−) | 813 | 270 | 7.73 | 29,085.46 | Scaffold 12.166 |
AsJAZ10 | AsTIFY9a | 1 (−) | 552 | 183 | 8.46 | 20,269.01 | Scaffold 9.133 |
AsJAZ11 | AsTIFY5c | 4 (−) | 834 | 277 | 9.68 | 30,599.78 | Scaffold 5.102 |
AsJAZ12 | AsTIFY3a | 8 (−) | 666 | 221 | 6.61 | 23,444.4 | Scaffold 46.99 |
AsPPD1 | AsTIFY4a | 3 (+) | 987 | 328 | 6.46 | 35,656.83 | Scaffold 22.90 |
AsZIM1 | AsTIFY1a | 2 (+) | 915 | 304 | 6.17 | 32,875.36 | Scaffold 60.49 |
AsZIM2 | AsTIFY1b | 2 (+) | 855 | 284 | 6.65 | 30,628.17 | Scaffold 2.660 |
AsZML1 | AsTIFY2a | 2 (+) | 1041 | 346 | 4.94 | 38,095.15 | Scaffold 60.50 |
AsZML2 | AsTIFY2b | 2 (−) | 930 | 309 | 5.73 | 32,843.19 | Scaffold 2.738 |
AsTIFY8a | 8 (−) | 1212 | 403 | 9.66 | 42,728.3 | Scaffold 21.46 | |
AsTIFY8b | 4 (−) | 1254 | 417 | 8.53 | 44,097.7 | Scaffold 23.90 |
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Ma, Y.; Ran, J.; Li, G.; Wang, M.; Yang, C.; Wen, X.; Geng, X.; Zhang, L.; Li, Y.; Zhang, Z. Revealing the Roles of the JAZ Family in Defense Signaling and the Agarwood Formation Process in Aquilaria sinensis. Int. J. Mol. Sci. 2023, 24, 9872. https://doi.org/10.3390/ijms24129872
Ma Y, Ran J, Li G, Wang M, Yang C, Wen X, Geng X, Zhang L, Li Y, Zhang Z. Revealing the Roles of the JAZ Family in Defense Signaling and the Agarwood Formation Process in Aquilaria sinensis. International Journal of Molecular Sciences. 2023; 24(12):9872. https://doi.org/10.3390/ijms24129872
Chicago/Turabian StyleMa, Yimian, Jiadong Ran, Guoqiong Li, Mengchen Wang, Chengmin Yang, Xin Wen, Xin Geng, Liping Zhang, Yuan Li, and Zheng Zhang. 2023. "Revealing the Roles of the JAZ Family in Defense Signaling and the Agarwood Formation Process in Aquilaria sinensis" International Journal of Molecular Sciences 24, no. 12: 9872. https://doi.org/10.3390/ijms24129872