Sertindole, an Antipsychotic Drug, Curbs the STAT3/BCL-xL Axis to Elicit Human Bladder Cancer Cell Apoptosis In Vitro
Abstract
:1. Introduction
2. Results
2.1. Sertindole Is Cytotoxic to Various Human Urinary Bladder TCC Cell Lines While Showing Less Cytotoxicity to Normal Human Urothelial Cells
2.2. Sertindole-Induced Bladder Cancer Cytotoxicity Depends on Apoptosis Induction
2.3. Suppression of STAT3 Activation Is Pivotal to Sertindole-Induced Human Bladder Cancer Apoptosis
2.4. BCL-xL Downregulation Is Responsible for Human Bladder Cell Apoptosis Resulting from Sertindole-Induced STAT3 Inhibition
2.5. Neither JAK2 nor SRC Appears to Involve in Sertindole-Inhibited STAT3 Activation
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Plasmids
4.3. Cell Culture
4.4. Cytotoxicity Assay
4.5. Immunoblotting
4.6. Apoptosis Assay
4.7. Establishment of HA-STAT3-C and HA-BCL-xL Stable Clones in TCCSUP and T24 Cells
4.8. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Hsu, C.-Y.; Yang, W.-T.; Lin, J.-H.; Lu, C.-H.; Hu, K.-C.; Lan, T.-H.; Chang, C.-C. Sertindole, an Antipsychotic Drug, Curbs the STAT3/BCL-xL Axis to Elicit Human Bladder Cancer Cell Apoptosis In Vitro. Int. J. Mol. Sci. 2023, 24, 11852. https://doi.org/10.3390/ijms241411852
Hsu C-Y, Yang W-T, Lin J-H, Lu C-H, Hu K-C, Lan T-H, Chang C-C. Sertindole, an Antipsychotic Drug, Curbs the STAT3/BCL-xL Axis to Elicit Human Bladder Cancer Cell Apoptosis In Vitro. International Journal of Molecular Sciences. 2023; 24(14):11852. https://doi.org/10.3390/ijms241411852
Chicago/Turabian StyleHsu, Chao-Yu, Wei-Ting Yang, Ju-Hwa Lin, Chien-Hsing Lu, Kai-Cheng Hu, Tsuo-Hung Lan, and Chia-Che Chang. 2023. "Sertindole, an Antipsychotic Drug, Curbs the STAT3/BCL-xL Axis to Elicit Human Bladder Cancer Cell Apoptosis In Vitro" International Journal of Molecular Sciences 24, no. 14: 11852. https://doi.org/10.3390/ijms241411852