Acute Exposure to Microplastics Induced Changes in Behavior and Inflammation in Young and Old Mice
Abstract
:1. Introduction
2. Results
2.1. Cell Viability and Cellular Uptake
2.2. In Vivo Exposure and Behavioral Studies
2.3. Bioaccumulation of MPs
2.4. Assessing Impact of MPs on Immune Markers
3. Discussion
4. Materials and Methods
4.1. Microplastic Particles
4.2. MTT Assay
4.3. Visualization of Red Fluorescent MPs in Cells
4.4. Animals and Exposure
4.5. Behavior Experiments
4.6. Open-Field Test (OF)
4.7. Light–Dark Preference Test (LD)
4.8. Tissue Preparation
4.9. Visualization of Red Fluorescent MPs in Tissues
4.10. Fluorescent Immunohistochemistry (IHC)
4.11. Western Blot (WB)
4.12. Quantitative Real-Time PCR (qPCR)
4.13. Microscopy
4.14. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gaspar, L.; Bartman, S.; Coppotelli, G.; Ross, J.M. Acute Exposure to Microplastics Induced Changes in Behavior and Inflammation in Young and Old Mice. Int. J. Mol. Sci. 2023, 24, 12308. https://doi.org/10.3390/ijms241512308
Gaspar L, Bartman S, Coppotelli G, Ross JM. Acute Exposure to Microplastics Induced Changes in Behavior and Inflammation in Young and Old Mice. International Journal of Molecular Sciences. 2023; 24(15):12308. https://doi.org/10.3390/ijms241512308
Chicago/Turabian StyleGaspar, Lauren, Sydney Bartman, Giuseppe Coppotelli, and Jaime M. Ross. 2023. "Acute Exposure to Microplastics Induced Changes in Behavior and Inflammation in Young and Old Mice" International Journal of Molecular Sciences 24, no. 15: 12308. https://doi.org/10.3390/ijms241512308