Reducing Sialylation Enhances Electrotaxis of Corneal Epithelial Cells
Abstract
:1. Introduction
2. Results
2.1. Reducing Sialylation Enhances Cathodal Migration of hTCEpi Cells
2.2. Reducing Sialylation May Enhance the Sensitivity of hTCEpi Cells to the Electric Field
2.3. Increasing Sialylation Impairs Electrotaxis
2.4. Pretreating hTCEpi Cells with Kifunensine Enhances Electrotaxis
2.5. Pretreating Enucleated Eyes with 3F-Neu5Ac Promotes Re-Epithelialization in an Ex Vivo Model of Corneal Injury
2.6. hTCEpi Cells Modulate Sialylations in Response to PDGF-BB and Growth Factor Starvation
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Cell Culture
4.3. Flow Cytometry
4.4. Electrotaxis
4.5. Ex Vivo Corneal Wound Healing
4.6. Gene Expression Analysis
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Le, B.; Zhu, K.; Brown, C.; Reid, B.; Cressman, A.; Zhao, M.; Fierro, F.A. Reducing Sialylation Enhances Electrotaxis of Corneal Epithelial Cells. Int. J. Mol. Sci. 2023, 24, 14327. https://doi.org/10.3390/ijms241814327
Le B, Zhu K, Brown C, Reid B, Cressman A, Zhao M, Fierro FA. Reducing Sialylation Enhances Electrotaxis of Corneal Epithelial Cells. International Journal of Molecular Sciences. 2023; 24(18):14327. https://doi.org/10.3390/ijms241814327
Chicago/Turabian StyleLe, Bryan, Kan Zhu, Chelsea Brown, Brian Reid, Amin Cressman, Min Zhao, and Fernando A. Fierro. 2023. "Reducing Sialylation Enhances Electrotaxis of Corneal Epithelial Cells" International Journal of Molecular Sciences 24, no. 18: 14327. https://doi.org/10.3390/ijms241814327