TBM Hunter: Identify and Score Canonical, Extended, and Unconventional Tankyrase-Binding Motifs in Any Protein
Abstract
:1. Introduction
2. Results
2.1. Classification of Three Types of TBMs
2.2. Building a Web-Based Tool, TBM Hunter, for Identifying and Scoring Various TBMs
2.2.1. Search Canonical and Extended TBMs with “Find and Score Motifs!”
2.2.2. Score TBMs with “Find and Score Motifs!” and “Score Any 8-Amino-Acid Sequence”
2.3. TBM Hunter Identified and Scored Known Canonical TBMs
2.4. TBM Hunter Identified and Scored Known Extended TBMs
2.5. TBM Hunter Scored Unconventional TBMs
2.6. TBM Hunter Discovered Novel TBMs in SASH1, Which Were Experimentally Validated
3. Discussion
4. Materials and Methods
4.1. Computer Program Languages and the Devleopment of TBM Hunter
4.2. Protocol for Identifying and Scoring Canonical and Extended TBMs Using the “Find and Score Motifs!” Function
4.3. Protocol for Scoring Unconventional TBMs Using the “Score Any 8-Amino Acid Sequence” Function
4.4. Scoring Systems for TBMs
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Sequence | Scoring | |
---|---|---|
Canonical TBM | R1-x-x-x-x-G6-(no-P)-x | All residues |
R1-x-x-x-x-x-G7-(no-P)-x | ||
Extended TBM | R1-x-x-x-x-x-x-G8-(no-P)-x | R1, i − 2, i − 1, i, i + 1, i + 2 (i = G7, G8, or G9) |
R1-x-x-x-x-x-x-x-G9-(no-P)-x | ||
Unconventional TBM | x-x-x-x-x-G6-x-x | All residues |
Protein | UniProt Code | Motif Seq | Starting Position | Score | References |
---|---|---|---|---|---|
3BP2 | P78314 | RSPPDGQS | 415 | 0.80 | [11] |
MCL1 | Q07820 | RNAVIGLN | 6 | 0.45 | |
RREIGGGE | 45 | 0.35 | |||
REIGGGEA | 46 | 0.65 | |||
RPPPIGAE | 78 | 0.69 | [11,16] | ||
REQATGAK | 187 | 0.48 | |||
RRVGDGVQ | 214 | 0.89 | |||
NUMA1 | Q14980 | RAEELGQE | 1321 | 0.37 | |
RTQPDGTS | 1743 | 0.79 | [11,17] | ||
RDRHEGRK | 2017 | 0.46 | |||
AXIN1 | O15169 | RPPVPGEE | 22 | 0.38 | [11,18] |
RRYSEGRE | 284 | 0.49 | |||
RMEEEGED | 417 | 0.54 | |||
RRTGHGSS | 647 | 0.61 | |||
RKVGGGSA | 770 | 0.61 | |||
TERF1 | P54274 | RGCADGRD | 13 | 0.78 | [11,17] |
RAFRDGRS | 88 | 0.45 | |||
RKYGEGNW | 396 | 0.76 | |||
FAT4 | Q6V0I7 | RLQDEGTP | 288 | 0.48 | |
RCVPPGDC | 4423 | 0.49 | |||
RKQPEGNP | 4572 | 0.64 | [11] | ||
RHSPLGFA | 4653 | 0.49 | |||
RNPADGIP | 4827 | 0.76 | [11] | ||
DISC1 | Q9NRI5 | RARQCGLD | 82 | 0.48 | |
RVRAAGSL | 170 | 0.46 | |||
RGEAEGCP | 223 | 0.65 | [11] | ||
REGLEGLL | 618 | 0.47 | |||
BABA1 | Q9NWV8 | RSNPEGAE | 28 | 0.70 | [11] |
RSEGEGEA | 48 | 0.82 | [11] | ||
LKB1 | Q15831 | RAKLIGKY | 42 | 0.44 | [19] |
RRIPNGEA | 86 | 0.50 | [19] | ||
AMPK | P54619 | N/A | N/A | N/A | [19] |
Protein | UniProt Code | Extended TBM Seq | Starting Position | Score |
---|---|---|---|---|
RNF146 | Q9NTX7 | RESSADGAD | 194 | 0.54 |
RPLTSVDGQL | 220 | 0.34 | ||
RSHRGEGEE | 260 | 0.59 | ||
RSVAGGGTV | 333 | 0.35 | ||
RSRRPDGQC | 346 | 0.53 | ||
IF4A1 | P60842 | RSRDNGPDGME | 8 | 0.57 |
RDNGPDGME | 10 | 0.57 | ||
RGIYAYGFE | 45 | 0.38 | ||
RAILPCIKGYD | 61 | 0.06 | ||
RENYIHRIGRG | 353 | 0.17 | ||
RIGRGGRFGRK | 359 | 0.01 | ||
RGGRFGRKGVA | 362 | 0.03 | ||
NELFE | P18615 | RSRTLEGKL | 88 | 0.19 |
RLRELGPDGEE | 142 | 0.59 | ||
RELGPDGEE | 144 | 0.59 | ||
RDRDRDREGPF | 236 | 0.04 | ||
RDRDREGPF | 238 | 0.04 | ||
RRAPRKGNT | 255 | 0.02 | ||
RGAFSPFGNI | 277 | 0.21 |
Protein | Motif Seq | Score |
---|---|---|
EBNA1 | EGGPDGEE | 0.44 |
GRB14 | LPLPDGTR | 0.38 |
Starting Position | Motif Seq | Score | Type | Validation |
---|---|---|---|---|
404 | RTCSFGGF | 0.30 | Canonical | Yes |
416 | RSLHVGSN | 0.46 | Canonical | Yes |
512 | RSSLSGQS | 0.33 | Canonical | Yes |
552 | RGPFCGRA | 0.46 | Canonical | Yes |
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Clements, C.M.; Shellman, S.X.; Shellman, M.H.; Shellman, Y.G. TBM Hunter: Identify and Score Canonical, Extended, and Unconventional Tankyrase-Binding Motifs in Any Protein. Int. J. Mol. Sci. 2023, 24, 16964. https://doi.org/10.3390/ijms242316964
Clements CM, Shellman SX, Shellman MH, Shellman YG. TBM Hunter: Identify and Score Canonical, Extended, and Unconventional Tankyrase-Binding Motifs in Any Protein. International Journal of Molecular Sciences. 2023; 24(23):16964. https://doi.org/10.3390/ijms242316964
Chicago/Turabian StyleClements, Christopher M., Samantha X. Shellman, Melody H. Shellman, and Yiqun G. Shellman. 2023. "TBM Hunter: Identify and Score Canonical, Extended, and Unconventional Tankyrase-Binding Motifs in Any Protein" International Journal of Molecular Sciences 24, no. 23: 16964. https://doi.org/10.3390/ijms242316964