Endoplasmic Reticulum Protein TXNDC5 Interacts with PRDX6 and HSPA9 to Regulate Glutathione Metabolism and Lipid Peroxidation in the Hepatic AML12 Cell Line
Abstract
:1. Introduction
2. Results
2.1. Identification of PRDX6 and HSPA9 as Novel TXNDC5-Interacting Proteins in the AML12 Cell Line
2.2. HSPA9 mRNA and Protein Levels Are Differentially Influenced by the Absence of TXNDC5
2.3. TXNDC5 Absence Reduces PRDX6 Protein Levels
2.4. Proteasomes Are Not Involved in the Reduction of PRDX6 Protein Induced by the Absence of TXNDC5
2.5. Lipid Peroxidation Was Decreased in the Absence of TXNDC5
2.6. TXNDC5 Deficiency Decreases Reduced Glutathione and IPLA2 Activity
2.7. HSPA9 and PRDX6 Target Genes
2.8. Hepatic Gene Expression
3. Discussion
4. Materials and Methods
4.1. AML12 Cell Culture
4.2. Creation of a Stable TXNDC5 Knockout AML12 Cell Line
4.3. Co-Immunoprecipitation (Co-IP) Assay
4.4. Protein Identification via LC-MS/MS
4.5. Western Blot
4.6. Bioinformatic Analyses
4.7. RNA Extraction
4.8. RNAseq Analyses
4.9. Reverse Transcription and Quantitative Real-Time PCR
4.10. Proteasome Inhibition in AML12 WT and KO Cells
4.11. Determination of Cellular Lipid Peroxidation via MDA Assay
4.12. Total Phospholipase A2 (PLA2) and Calcium-Independent Phospholipase A2 (iPLA2) Activity Assays
4.13. Intracellular Reduced Glutathione (GSH) Concentration Determination
4.14. Statistical Analysis
5. Conclusions
Limitations and Future Research
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Symbol | Gene ID (NCBI) | SL2R RNAseq (AML12) | Regulation (AML12) KO/WT |
---|---|---|---|
Dnaja3 [31] | 83945 | 1.2331 | UP |
Hsp90aa1 [32] | 15519 | −1.2332 | DOWN |
Mfn2 [33] | 170731 | 1.6794 | UP |
Rps14 [31] | 20044 | −1.6093 | DOWN |
Gene Symbol | Gene ID (NCBI) | SL2R RNAseq (AML12) | Regulation (AML12) KO/WT |
---|---|---|---|
Gstm6 | 14867 | −2.1758 | DOWN |
Gstp3 | 225884 | −2.6291 | DOWN |
Gstt1 | 14871 | −3.3955 | DOWN |
Npm1 [34] | 18148 | −2.3854 | DOWN |
Oplah | 75475 | −2.5423 | DOWN |
Prdx5 [35] | 54683 | −2.0351 | DOWN |
Serpina1a [36] | 20700 | −3.8105 | DOWN |
Serpina1b [36] | 20701 | −2.5978 | DOWN |
Serpina3m [37] | 20717 | −6.7645 | DOWN |
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Bidooki, S.H.; Sánchez-Marco, J.; Martínez-Beamonte, R.; Herrero-Continente, T.; Navarro, M.A.; Rodríguez-Yoldi, M.J.; Osada, J. Endoplasmic Reticulum Protein TXNDC5 Interacts with PRDX6 and HSPA9 to Regulate Glutathione Metabolism and Lipid Peroxidation in the Hepatic AML12 Cell Line. Int. J. Mol. Sci. 2023, 24, 17131. https://doi.org/10.3390/ijms242417131
Bidooki SH, Sánchez-Marco J, Martínez-Beamonte R, Herrero-Continente T, Navarro MA, Rodríguez-Yoldi MJ, Osada J. Endoplasmic Reticulum Protein TXNDC5 Interacts with PRDX6 and HSPA9 to Regulate Glutathione Metabolism and Lipid Peroxidation in the Hepatic AML12 Cell Line. International Journal of Molecular Sciences. 2023; 24(24):17131. https://doi.org/10.3390/ijms242417131
Chicago/Turabian StyleBidooki, Seyed Hesamoddin, Javier Sánchez-Marco, Roberto Martínez-Beamonte, Tania Herrero-Continente, María A. Navarro, María J. Rodríguez-Yoldi, and Jesús Osada. 2023. "Endoplasmic Reticulum Protein TXNDC5 Interacts with PRDX6 and HSPA9 to Regulate Glutathione Metabolism and Lipid Peroxidation in the Hepatic AML12 Cell Line" International Journal of Molecular Sciences 24, no. 24: 17131. https://doi.org/10.3390/ijms242417131