Single-Stage Microfluidic Synthesis Route for BaGdF5:Tb3+-Based Nanocomposite Materials: Synthesis, Characterization and Biodistribution
Abstract
:1. Introduction
2. Results and Discussion
2.1. Adapting Reaction Conditions in MF Mode
2.2. Synthesis of BaGd1−xF5:Tbx3+ Nanophosphors with In-Situ XEOL Acquisition
2.3. Single-Stage Microfluidic Synthesis of BaGdF5:Tb@RoseBengal Nanocomposite
2.4. Cytotoxicity and Flow Cytometry Results
2.5. Long-Term Monitoring of Nanocomposite Biodistribution via Micro-CT
3. Materials and Methods
3.1. Microfluidic Setup and Microreactors
3.2. X-ray-Excited Optical Luminescence
3.3. Other Characterization Techniques
3.4. Cytotoxicity Test
3.5. Flow Cytometry
3.6. Statistical Analysis
3.7. Micro-CT In Vivo Diagnostics
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Solvent Composition | |
X% of EG | Particle concentration, mg/mL |
100 EG | 1.5–2 |
75 EG | 10–12 |
50 EG | chip clogging |
25 EG | chip clogging |
0 EG | chip clogging |
Synthesis time (total flow rates) | |
Time | Particle concentration, mg/mL |
2 h | chip clogging |
1 h | 10–12 |
30 min | 10–12 |
12 min | 10–12 |
6 min | 10–12 |
Metals chloride (precursors) concentration * | |
Concentration, mol/L | Particle concentration, mg/mL |
0.1 | 10–12 |
0.2 | 17–19 |
0.3 | 25–30 |
0.4 | chip clogging |
0.5 | chip clogging |
Sample | Precursor Flow Rates, μL/s | |||
---|---|---|---|---|
Ba2+ | Gd3+ | Tb3+ | F− | |
5Tb | 10.28 | 9.77 | 0.51 | 10.28 |
10Tb | 9.25 | 1.03 | ||
15Tb | 8.74 | 1.54 | ||
20Tb | 8.22 | 2.06 | ||
25Tb | 7.71 | 2.57 |
Sample | Expected Tb Content, at.% | Actual Tb Content, at.% | Cell Parameters, Å | Cell Volume, Å3 | Goodness of Fit (GOF) | R-Factor |
---|---|---|---|---|---|---|
5Tb | 0.71 | 0.66 | 5.9388 (14) | 209.46 (8) | 1.06 | 0.1506 |
10Tb | 1.43 | 1.29 | 5.9408 (14) | 209.67 (9) | 1.06 | 0.1449 |
15Tb | 2.13 | 2.11 | 5.9355 (12) | 209.11 (7) | 1.03 | 0.1275 |
20Tb | 2.86 | 2.86 | 5.9352 (16) | 209.08 (10) | 1.08 | 0.18 |
25Tb | 3.57 | 3.63 | 5.9337 (11) | 208.92 (7) | 1.01 | 0.1217 |
Sample | A549 | HSF | HeLa |
---|---|---|---|
Control | 28.156 ± 8.823 | 32.261 ± 12.467 | 31.060 ± 10.525 |
NPs@RB | 40.398 ± 13.794 * | 52.504 ± 31.848 * | 32.343 ± 19.897 |
NPs | 45.946 ± 13.134 * | 60.513 ± 30.340 * | 37.061 ± 15.484 * |
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Gadzhimagomedova, Z.; Pankin, I.; Polyakov, V.; Khodakova, D.; Medvedev, P.; Zelenikhin, P.; Shamsutdinov, N.; Chapek, S.; Goncharova, A.; Soldatov, A. Single-Stage Microfluidic Synthesis Route for BaGdF5:Tb3+-Based Nanocomposite Materials: Synthesis, Characterization and Biodistribution. Int. J. Mol. Sci. 2023, 24, 17159. https://doi.org/10.3390/ijms242417159
Gadzhimagomedova Z, Pankin I, Polyakov V, Khodakova D, Medvedev P, Zelenikhin P, Shamsutdinov N, Chapek S, Goncharova A, Soldatov A. Single-Stage Microfluidic Synthesis Route for BaGdF5:Tb3+-Based Nanocomposite Materials: Synthesis, Characterization and Biodistribution. International Journal of Molecular Sciences. 2023; 24(24):17159. https://doi.org/10.3390/ijms242417159
Chicago/Turabian StyleGadzhimagomedova, Zaira, Ilia Pankin, Vladimir Polyakov, Darya Khodakova, Pavel Medvedev, Pavel Zelenikhin, Nail Shamsutdinov, Sergey Chapek, Anna Goncharova, and Alexander Soldatov. 2023. "Single-Stage Microfluidic Synthesis Route for BaGdF5:Tb3+-Based Nanocomposite Materials: Synthesis, Characterization and Biodistribution" International Journal of Molecular Sciences 24, no. 24: 17159. https://doi.org/10.3390/ijms242417159