Evaluation of the Antitumor Activity of Quaternary Ammonium Surfactants
Abstract
:1. Introduction
2. Results and Discussion
2.1. MTT Assay
2.2. Gene Expression (RT-qPCR)
2.3. Migration—Scratch Assay
3. Materials and Methods
3.1. Cell Culture Conditions
3.2. Cell Treatment
3.3. Preparation of Liposomes
3.4. MTT Assay
3.5. RNA Isolation and Transcript Quantification
3.6. Migration—Scratch Assay
3.7. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Size [nm] | Average Size [nm] | SD | |||
---|---|---|---|---|---|
DMM-11 | 206.8 | 196.7 | 183.2 | 195.56 | 11.84 |
DMPM-11 | 176.8 | 165.7 | 163.2 | 168.56 | 7.23 |
DMGM-14 | 95.10 | 94.27 | 93.63 | 94.33 | 0.73 |
PDI | Average Value | SD | |||
---|---|---|---|---|---|
DMM-11 | 0.2512 | 0.2244 | 0.2456 | 0.2404 | 0.0115 |
DMPM-11 | 0.2127 | 0.2081 | 0.2080 | 0.2096 | 0.0021 |
DMGM-14 | 0.1091 | 0.07784 | 0.1057 | 0.0975 | 0.0140 |
HT-29 | ||
β-actin | Forward | 5′CTGTCTGGCGGCACCACCAT3′ |
Reverse | 5′GCAACTAAGTCATAGTCCGC3′ | |
Bax | Forward | 5′AAGCTGAGCGAGTGTCTCAAGCGC3′ |
Reverse | 5′TCCCGCCACAAAGATGGTCACG3′ | |
Bcl-2 | Forward | 5′ATGGCAGCAGTAAAGCAAGCGC3′ |
Reverse | 5′TTCTCCTGGTGGCAATGGCG3′ | |
A375 | ||
GAPDH | Forward | 5′CAAGGTCATCCATGACAACTTTG3′ |
Reverse | 5′GTCCACCACCCTGTTGCTGTAG3′ | |
Bax | Forward | 5′CAGAACTGGACAGTAACATGGAG3′ |
Reverse | 5′CAGTTTGCTGGCAAAGTAGAAAAG3′ | |
Bcl-2 | Forward | 5′ATGTGTGTGGAGAGCGTCAA3′ |
Reverse | 5′GAGACAGCCAGGACAAATCAA3′ |
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Hyla, K.; Jama, D.; Grzywacz, A.; Janek, T. Evaluation of the Antitumor Activity of Quaternary Ammonium Surfactants. Int. J. Mol. Sci. 2023, 24, 17237. https://doi.org/10.3390/ijms242417237
Hyla K, Jama D, Grzywacz A, Janek T. Evaluation of the Antitumor Activity of Quaternary Ammonium Surfactants. International Journal of Molecular Sciences. 2023; 24(24):17237. https://doi.org/10.3390/ijms242417237
Chicago/Turabian StyleHyla, Kinga, Dominika Jama, Aleksandra Grzywacz, and Tomasz Janek. 2023. "Evaluation of the Antitumor Activity of Quaternary Ammonium Surfactants" International Journal of Molecular Sciences 24, no. 24: 17237. https://doi.org/10.3390/ijms242417237