Renal Expression and Localization of the Receptor for (Pro)renin and Its Ligands in Rodent Models of Diabetes, Metabolic Syndrome, and Age-Dependent Focal and Segmental Glomerulosclerosis
Abstract
:1. Introduction
2. Results
2.1. Kidney Specimens and Renal Structural and Functional Features of the Experimental Rodent Models
2.2. Immunostaining for FL-(P)RR
2.3. Immunostaining for (Pro)renin (Total Renin)
2.4. Immunostaining for COX-2
2.5. mRNA Levels of (P)RR and Factors Involved in Its Intracellular Processing
2.6. Impact of Elevated Glucose Concentration on (P)RR Expression and Intracellular Processing in Podocytes
3. Discussion
4. Materials and Methods
4.1. Immunohistochemical and Morphometric Analysis of Renal Sections
4.2. RT-PCR
4.3. Cell Culture and Treatment
4.4. Immunofluorescence Staining for FL-(P)RR in Cultured Human Podocytes
4.5. Enzyme-Linked Immunosorbent Assay (ELISA) for s(P)RR
4.6. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Diab Mice (4 Months of Diabetes) [16] | HFD-Fed Mice (4 Months of Fatty Diet) [17] | MNS Rats (9 Months Old) [14] | |
---|---|---|---|
Metabolic phenotype | severe hyperglycemia, insulinopenia (type 1 diabetes), body weight reduction, normal blood pressure | mild hyperglycemia, overweight, hyperinsulinemia, dyslipidemia, normal blood pressure | normal blood glucose, normal body weight, normal blood pressure |
Renal lesions | moderate diffuse glomerulosclerosis, increased GSI (↑↑), no tubular damage | mild diffuse glomerulosclerosis, increased GSI (↑), no tubular damage | severe segmental or global glomerulosclerosis, interstitial fibrosis, and tubular damage |
Proteinuria | 5 times than the control | 1.7 times than the control | 30 times than the control |
Target | Assay | ||
---|---|---|---|
Human | Mouse | Rat | |
ATP6AP2 | Hs00997145_m1 | Mm00510396_m1 | Rn01430719_m1 |
Furin | Hs00159829_m1 | Mm00440646_m1 | Rn00570970_m1 |
MBTPS1 | Hs00921626_m1 | Mm00490600_m1 | Rn00585707_m1 |
Mint3 | Hs01114376_m1 | Mm00444450_m1 | Rn00582358_m1 |
ACTB | Hs99999903_m1 | Mm02619580_m1 | Rn00667869_m1 |
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Iacobini, C.; Vitale, M.; Sentinelli, F.; Haxhi, J.; Pugliese, G.; Menini, S. Renal Expression and Localization of the Receptor for (Pro)renin and Its Ligands in Rodent Models of Diabetes, Metabolic Syndrome, and Age-Dependent Focal and Segmental Glomerulosclerosis. Int. J. Mol. Sci. 2024, 25, 2217. https://doi.org/10.3390/ijms25042217
Iacobini C, Vitale M, Sentinelli F, Haxhi J, Pugliese G, Menini S. Renal Expression and Localization of the Receptor for (Pro)renin and Its Ligands in Rodent Models of Diabetes, Metabolic Syndrome, and Age-Dependent Focal and Segmental Glomerulosclerosis. International Journal of Molecular Sciences. 2024; 25(4):2217. https://doi.org/10.3390/ijms25042217
Chicago/Turabian StyleIacobini, Carla, Martina Vitale, Federica Sentinelli, Jonida Haxhi, Giuseppe Pugliese, and Stefano Menini. 2024. "Renal Expression and Localization of the Receptor for (Pro)renin and Its Ligands in Rodent Models of Diabetes, Metabolic Syndrome, and Age-Dependent Focal and Segmental Glomerulosclerosis" International Journal of Molecular Sciences 25, no. 4: 2217. https://doi.org/10.3390/ijms25042217