Impact of Serotonergic 5HT1A and 5HT2A Receptor Activation on the Respiratory Response to Hypercapnia in a Rat Model of Parkinson’s Disease
Abstract
:1. Introduction
2. Results
2.1. Hypercapnic Ventilatory Response (HCVR) in PD and Sham Rats
2.2. HCVR in PD and Sham Rats before and after Intraperitoneal (i.p.) Treatment with 8-OH-DPAT
2.3. HCVR in PD and Sham Rats before and after i.p. Treatment with 25CN-NBOH
2.4. HCVR in PD and Sham Rats before and after i.p. Treatment with TCB-2
2.5. Comparison of HCVR in Sham and PD Rats after i.p. Treatment with 25CN-NBOH, TCB-2, and 8-OH-DPAT
2.6. Apnea Index Analysis
2.7. Concentration of Biogenic Amines in Striatum and Brainstem
3. Discussion
4. Material and Methods
4.1. Animals
4.2. Experimental Groups and Drug Treatment
- 25CN-NBOH at a dose of 1 mg kg−1 dissolved in water (Sham n = 7, 6-OHDA n = 7);
- TCB-2 at a dose of 1 mg kg−1 dissolved in water (Sham n = 7, 6-OHDA n = 7);
- 8-OH-DPAT at a dose of 0.5 mg kg−1 dissolved in water (Sham n = 6, 6-OHDA n = 6).
4.3. Surgery and 6-OHDA/Vehicle Injection
4.4. Ventilation Measurements
4.5. Apnea Measurement
4.6. High-Performance Liquid Chromatography (HPLC) Analysis
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Andrzejewski, K.; Orłowska, M.E.; Zaremba, M.; Joniec-Maciejak, I.; Kaczyńska, K. Impact of Serotonergic 5HT1A and 5HT2A Receptor Activation on the Respiratory Response to Hypercapnia in a Rat Model of Parkinson’s Disease. Int. J. Mol. Sci. 2024, 25, 4403. https://doi.org/10.3390/ijms25084403
Andrzejewski K, Orłowska ME, Zaremba M, Joniec-Maciejak I, Kaczyńska K. Impact of Serotonergic 5HT1A and 5HT2A Receptor Activation on the Respiratory Response to Hypercapnia in a Rat Model of Parkinson’s Disease. International Journal of Molecular Sciences. 2024; 25(8):4403. https://doi.org/10.3390/ijms25084403
Chicago/Turabian StyleAndrzejewski, Kryspin, Magdalena E. Orłowska, Małgorzata Zaremba, Ilona Joniec-Maciejak, and Katarzyna Kaczyńska. 2024. "Impact of Serotonergic 5HT1A and 5HT2A Receptor Activation on the Respiratory Response to Hypercapnia in a Rat Model of Parkinson’s Disease" International Journal of Molecular Sciences 25, no. 8: 4403. https://doi.org/10.3390/ijms25084403