Identification of Indicator Genes for Agar Accumulation in Gracilariopsis lemaneiformis (Rhodophyta)
Abstract
:1. Introduction
2. Results
2.1. Growth Assessment
2.2. Agar Content
2.3. Soluble Polysaccharides Content
2.4. The Transcription Level of 11 Genes in G. lemaneiformis under Four Treatments
2.5. The Variation of Relationship between Agar Content and Gene Transcription Level in Different Varieties and Generations of G. lemaneiformis
2.6. The Relationship between Agar Content and Gene Transcription Level
3. Discussion
4. Materials and Methods
4.1. Algae and Homogenisation Cultivation
4.2. Cultivation under Four Treatments
4.3. Determination of Relative Growth Rate
4.4. Determination of Agar Content
4.5. Measurement of Soluble Polysaccharides Content
4.6. RNA Extraction and cDNA Synthesis
4.7. Analysis of Genes Transcription Levels
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Genes | gpiI | gpiII | pgm | ugp | galt | mpi | pmm | mpg | gme | gatI | gatII |
---|---|---|---|---|---|---|---|---|---|---|---|
PCC | 0.503 | 0.925 * | −0.278 | −0.023 | 0.742 | 0.776 * | −0.532 | 0.901 * | −0.636 | 0.209 | 0.856 * |
Sample Collection Time | Treatments | Groups (Levels) | ||||
---|---|---|---|---|---|---|
13 August 2022 | Salinity (‰) | 10 | 20 | 30 (control) | 40 | - |
3 September 2022 | Temperature (°C) | 5 | 10 | 20 (control) | 30 | 35 |
12 October 2022 | N (µM) | 6 | 30 (control) | 300 | 900 | - |
11 November 2022 | P (µM) | 0.5 | 1.5 (control) | 30 | - | - |
Component | Concentration (g/L) |
---|---|
NaCl | 24.470 |
Na2SO4 | 3.9170 |
KCl | 0.6640 |
KBr | 0.0960 |
SrCl2·6H2O | 0.0402 |
MgCl·6H2O | 4.9810 |
CaCl2 | 0.9480 |
NaHCO3 | 0.1920 |
NaF | 0.0039 |
NaH2PO4·2H2O | X |
H3BO3 | 0.0260 |
NaNO3 | Y |
Na2SiO3·9H2O | 0.0230 |
Treatments | X, g/L (µM) | Y, g/L (µM) |
---|---|---|
Various N Concentrations | 4.68 × 10−3 (30) | 5.10 × 10−5 (6) |
2.55 × 10−3 (30) | ||
2.55 × 10−2 (300) | ||
7.65 × 10−2 (900) | ||
Various P Concentrations | 7.80 × 10−5 (0.5) | 2.55 × 10−2 (300) |
2.35 × 10−4 (1.5) | ||
4.68 × 10−3 (30) |
Genes Name | Forward Primers (5′–3′) | Reverse Primer (5′–3′) | PCR Products (bp) |
---|---|---|---|
gpiI | TGCTCCAACTTGCTGCCGA | GCTCGCAGGGCGGTATGAA | 193 |
gpiII | ACCACCGCCGAAACCATGC | CCAGTCCCAGAATCCAAACACG | 165 |
pgm | TCCTTCTGATTCTGTCGCTGTC | ATTTCCAACCTGTAGGCACTTC | 167 |
ugp | GACCTTATCGTCCAGCAAATCG | CCTTGACAATCCTCGGGTAGCG | 181 |
galt | CGTTCCTTTGCTTCCATTTCG | TCTGGGCATCTGAGTTCGTTC | 127 |
mpi | CTCCGCCGACTCCGTTCAAGA | TACGCCGCAAAGCAGCCCACAT | 138 |
pmm | CGTTCTGGGATTACGGTCTC | GCATAGTTTCTTCCGGGTTC | 219 |
mpg | CCTTGATTCTCGTCGGTGGTTA | GCATCTTCTCGGGCTGGTAGTT | 174 |
gme | TGGGTAAGATGGTGCTCGGATTT | TCAAAAGTCCTCCTGAGCCCGT | 163 |
gatI | GCTGGATTTGTAATGTTGATGTTGC | TGGATAAGCCTCGTGCGTTC | 211 |
gatII | CCTTGAGCTGGTTGTGCAATGT | CATGAGCATCCAGTATCCCG | 233 |
act | CTACTCGTTTACCACTTCTGCTGA | TTCCATTCCGACCAACTCTG | 220 |
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Li, Z.; Tu, M.; Qin, F.; Shui, G.; Xu, D.; Zang, X. Identification of Indicator Genes for Agar Accumulation in Gracilariopsis lemaneiformis (Rhodophyta). Int. J. Mol. Sci. 2024, 25, 4606. https://doi.org/10.3390/ijms25094606
Li Z, Tu M, Qin F, Shui G, Xu D, Zang X. Identification of Indicator Genes for Agar Accumulation in Gracilariopsis lemaneiformis (Rhodophyta). International Journal of Molecular Sciences. 2024; 25(9):4606. https://doi.org/10.3390/ijms25094606
Chicago/Turabian StyleLi, Zheng, Mengge Tu, Feng Qin, Guangqiang Shui, Di Xu, and Xiaonan Zang. 2024. "Identification of Indicator Genes for Agar Accumulation in Gracilariopsis lemaneiformis (Rhodophyta)" International Journal of Molecular Sciences 25, no. 9: 4606. https://doi.org/10.3390/ijms25094606