SELDI-TOF-MS Analysis of Transcriptional Activation Protein Binding to Response Elements Regulating Carcinogenesis Enzymes
Abstract
:Introduction
Methods
AP-1consensus forward: 5’-[NH2-(CH2)12]-CGCTTGATGAGTCAGCCGGAA-3’ AP-1 consensus reverse: 3’-GCGAACTACTCAGTCGGCCTT-5’ AP-1scrambled forward: 5’-[NH2-(CH2)12]-CAGGCGTGTCTGACTACAGAG-3’ AP-1 scrambled reverse: 3’-GTCCGCACAGACTGATGTCTC-5’Complementary oligonucleotides were annealed at a hybridized concentration of 25 µM in 10 mM K2HPO4/KH2PO4, 10 mM MgCl2 buffer (pH 7.4) at 90o C, 5 min, cooled to ambient temperature for 30 min and stored at 4º C. Annealing buffer was adjusted to pH 9.0 for coupling of the hybridized DNA to the PS20 protein chip array epoxide surface (Ciphergen Biosystems, Fremont, CA) via reaction of the 5'- amine on the leading oligonucleotide to the activated epoxy groups on the chip surface. To each spot 250 pmol oligonucleotide was applied in 10 µL and incubated at 37o C for 1 h in a humid chamber. To block residual active sites on the chip surface, 4 µL 1 M ethanolamine, pH 8.0 in annealing buffer was added for 30 min at 37º C , then washed with buffer (100mM Tris, 250 mM NaCl, 1mM EDTA, pH 7.4).
Results and Discussion
Determination of linearity and saturation of chip surface with oligonucleotide
Detection limits for recombinant c-Jun
Effect of reducing conditions on binding of c-Jun to oligonucleotide
Application of experimental conditions to human cell nuclear protein extracts
Acknowledgments
References
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Bischoff, S.R.; Kahn, M.B.; Powell, M.D.; Kirlin, W.G. SELDI-TOF-MS Analysis of Transcriptional Activation Protein Binding to Response Elements Regulating Carcinogenesis Enzymes. Int. J. Mol. Sci. 2002, 3, 1027-1038. https://doi.org/10.3390/i3101027
Bischoff SR, Kahn MB, Powell MD, Kirlin WG. SELDI-TOF-MS Analysis of Transcriptional Activation Protein Binding to Response Elements Regulating Carcinogenesis Enzymes. International Journal of Molecular Sciences. 2002; 3(10):1027-1038. https://doi.org/10.3390/i3101027
Chicago/Turabian StyleBischoff, Steve R., Mahfuz B. Kahn, Michael D. Powell, and Ward G. Kirlin. 2002. "SELDI-TOF-MS Analysis of Transcriptional Activation Protein Binding to Response Elements Regulating Carcinogenesis Enzymes" International Journal of Molecular Sciences 3, no. 10: 1027-1038. https://doi.org/10.3390/i3101027