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Sensors, Volume 1, Issue 2 (July 2001), Pages 53-74

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Research

Open AccessArticle Assembly method fabricating linkers for covalently bonding DNA on glass surface
Sensors 2001, 1(2), 53-59; doi:10.3390/s10100053
Received: 13 June 2001 / Accepted: 14 July 2001 / Published: 20 July 2001
Cited by 8 | PDF Full-text (110 KB)
Abstract
A fabrication method of linkers to covalently anchor nucleic acid probes (such as, oligonucleotides, PCR-products or peptide nucleic acid oligomers) on glass was developed by alternatively covalently assembling the different molecular components. The linkers with controllable length, flexibility and hydrophilicity could be prepared
[...] Read more.
A fabrication method of linkers to covalently anchor nucleic acid probes (such as, oligonucleotides, PCR-products or peptide nucleic acid oligomers) on glass was developed by alternatively covalently assembling the different molecular components. The linkers with controllable length, flexibility and hydrophilicity could be prepared simply by dipping subtracts alternatively in different chemical solutions. A dialkoxy aminosilane (N-(2-aminoethyl)-3-aminopropylmethyl dimethoxylsilane, AEAPS) was chosen to substitute common used trialkoxy aminosilane to modify glass surface. The end groups of the linkers were formed as aldehyde or amino group, which were successfully used for attaching prefabricated DNA or in situ synthesis of oligonucleotides, respectively. The both experiments showed that the linker produce good reproducibility and uniformity of fluorescent hybridizing images, which can distinguish an internal single base mismatch in 20mer oligonucleotides. Full article
Open AccessArticle New Trends in Biosensors for Organophosphorus Pesticides
Sensors 2001, 1(2), 60-74; doi:10.3390/s10100060
Received: 13 July 2001 / Accepted: 18 July 2001 / Published: 24 July 2001
Cited by 68 | PDF Full-text (143 KB) | HTML Full-text | XML Full-text
Abstract
Biosensors are sensitive and can be used as disposable sensors for environmental control. These biosensors are based either on inhibition of acyl cholinesterases acetylcholinesterase or butyrylcholinesterase) by organophosphorus compounds or on inhibition of enzymes phosphatases (acid or alkaline) or on direct detection of
[...] Read more.
Biosensors are sensitive and can be used as disposable sensors for environmental control. These biosensors are based either on inhibition of acyl cholinesterases acetylcholinesterase or butyrylcholinesterase) by organophosphorus compounds or on inhibition of enzymes phosphatases (acid or alkaline) or on direct detection of organophosphorus compounds by organophosphorus hydrolase. The state-of-the-art of the three types of biosensors will be presented in this paper and a particular development will be done on our work on ENFET (Enzymatic Field Effect Transistor) biosensors. Full article

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