A Novel Biosensor for Evaluation of Apoptotic or Necrotic Effects of Nitrogen Dioxide during Acute Pancreatitis in Rat
Abstract
:1. Introduction
2. Results and Disscusion
2.1. Kinetics of •NO2 Uptake Generated from Pancreatic Cytosol Fractions
2.2. Biological Study
2.3. Discussion
3. Materials and Methods
3.1. Reagents
3.2. Animals-Experimental Groups
- control rats that received 0.9% NaCl (ip) (n = 8),
- rats injected (ip) with L-arg/HCl solution (pH = 7.4) at a dose of 1.5 g of free amino acid per 1 kg bw (n = 8),
- rats injected (ip) with L-arg/HCl solution (pH = 7.4) at a dose of 3.0 g of free amino acid per 1 kg bw (n = 8),
- rats injected (ip) with 20 μg 4-OH-TEMPO dissolved in 0.9% NaCl; 20 min. later the rats were injected (ip) with L-arg/HCl solution (pH = 7.4) at a dose of 1.5 g of free amino acid per 1 kg bw (n = 8),
- rats injected (ip) with 20μg 4-OH-TEMPO dissolved in 0.9% NaCl; 20 min. later the rats were injected (ip) with L-arg/HCl solution (pH = 7.4) at a dose of 3.0 g of free amino acid per 1 kg of bw (n = 8),
Sample preparation for •NO2 measurements
Samples for microscopic examination
3.3. Spectroscopic Measurements
3.4. Determination of Nitric Dioxide Concentration
3.5. Biochemical Assays
3.6. GSH Assessment
3.7. Statistical Analysis
4. Conclusions
Acknowledgments
References and Notes
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Jacewicz, D.; Dabrowska, A.; Wyrzykowski, D.; Pranczk, J.; Wozniak, M.; Kubasik-Juraniec, J.; Knap, N.; Siedlecka, K.; Neuwelt, A.J.; Chmurzynski, L. A Novel Biosensor for Evaluation of Apoptotic or Necrotic Effects of Nitrogen Dioxide during Acute Pancreatitis in Rat. Sensors 2010, 10, 280-291. https://doi.org/10.3390/s100100280
Jacewicz D, Dabrowska A, Wyrzykowski D, Pranczk J, Wozniak M, Kubasik-Juraniec J, Knap N, Siedlecka K, Neuwelt AJ, Chmurzynski L. A Novel Biosensor for Evaluation of Apoptotic or Necrotic Effects of Nitrogen Dioxide during Acute Pancreatitis in Rat. Sensors. 2010; 10(1):280-291. https://doi.org/10.3390/s100100280
Chicago/Turabian StyleJacewicz, Dagmara, Aleksandra Dabrowska, Dariusz Wyrzykowski, Joanna Pranczk, Michal Wozniak, Jolanta Kubasik-Juraniec, Narcyz Knap, Kamila Siedlecka, Alexander J. Neuwelt, and Lech Chmurzynski. 2010. "A Novel Biosensor for Evaluation of Apoptotic or Necrotic Effects of Nitrogen Dioxide during Acute Pancreatitis in Rat" Sensors 10, no. 1: 280-291. https://doi.org/10.3390/s100100280