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Sensors 2012, 12(2), 1544-1571; doi:10.3390/s120201544

Pseudomonas fluorescens HK44: Lessons Learned from a Model Whole-Cell Bioreporter with a Broad Application History

Faculty of the Environment, Jan Evangelista Purkyně University in Ústí nad Labem, Králova Výšina 3132/7, 400 96 Ústí nad Labem, Czech Republic
The Center for Environmental Biotechnology, The University of Tennessee, 676 Dabney Hall, Knoxville, TN 37996, USA
Institute of Chemical Process Fundamentals of ASCR, v.v.i., Rozvojová 135, 165 02, Prague 6-Suchdol, Czech Republic
Author to whom correspondence should be addressed.
Received: 29 December 2011 / Revised: 24 January 2012 / Accepted: 3 February 2012 / Published: 6 February 2012
(This article belongs to the Special Issue Live Cell-Based Sensors)
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Initially described in 1990, Pseudomonas fluorescens HK44 served as the first whole-cell bioreporter genetically endowed with a bioluminescent (luxCDABE) phenotype directly linked to a catabolic (naphthalene degradative) pathway. HK44 was the first genetically engineered microorganism to be released in the field to monitor bioremediation potential. Subsequent to that release, strain HK44 had been introduced into other solids (soils, sands), liquid (water, wastewater), and volatile environments. In these matrices, it has functioned as one of the best characterized chemically-responsive environmental bioreporters and as a model organism for understanding bacterial colonization and transport, cell immobilization strategies, and the kinetics of cellular bioluminescent emission. This review summarizes the characteristics of P. fluorescens HK44 and the extensive range of its applications with special focus on the monitoring of bioremediation processes and biosensing of environmental pollution. View Full-Text
Keywords: bioluminescence; bioreporter; biosensors; Pseudomonas fluorescens HK44; lux genes bioluminescence; bioreporter; biosensors; Pseudomonas fluorescens HK44; lux genes

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Trögl, J.; Chauhan, A.; Ripp, S.; Layton, A.C.; Kuncová, G.; Sayler, G.S. Pseudomonas fluorescens HK44: Lessons Learned from a Model Whole-Cell Bioreporter with a Broad Application History. Sensors 2012, 12, 1544-1571.

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