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Sensors 2013, 13(6), 7774-7785; doi:10.3390/s130607774

Impedimetric DNA Biosensor Based on a Nanoporous Alumina Membrane for the Detection of the Specific Oligonucleotide Sequence of Dengue Virus

Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore 637371, Singapore
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Received: 22 April 2013 / Revised: 7 June 2013 / Accepted: 13 June 2013 / Published: 17 June 2013
(This article belongs to the Special Issue Novel Tools in Electrochemical Sensing)
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Abstract

A novel and integrated membrane sensing platform for DNA detection is developed based on an anodic aluminum oxide (AAO) membrane. Platinum electrodes (~50–100 nm thick) are coated directly on both sides of the alumina membrane to eliminate the solution resistance outside the nanopores. The electrochemical impedance technique is employed to monitor the impedance changes within the nanopores upon DNA binding. Pore resistance (Rp) linearly increases in response towards the increasing concentration of the target DNA in the range of 1 × 10−12 to 1 × 10−6 M. Moreover, the biosensor selectively differentiates the complementary sequence from single base mismatched (MM-1) strands and non-complementary strands. This study reveals a simple, selective and sensitive method to fabricate a label-free DNA biosensor. View Full-Text
Keywords: biosensor; impedance; membrane; nanochannel; nanopore; dengue; infectious disease biosensor; impedance; membrane; nanochannel; nanopore; dengue; infectious disease
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Deng, J.; Toh, C.-S. Impedimetric DNA Biosensor Based on a Nanoporous Alumina Membrane for the Detection of the Specific Oligonucleotide Sequence of Dengue Virus. Sensors 2013, 13, 7774-7785.

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