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Sensors 2008, 8(3), 1497-1507; doi:10.3390/s8031497

Amperometric Low-Potential Detection of Malic Acid Using Single-Wall Carbon Nanotubes Based Electrodes

Laboratory of Quality Control and Process Monitoring, Department of Analytical Chemistry, University of Bucharest, 4-12 Regina Elisabeta Blvd., 030018 Bucharest, Romania
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Received: 4 February 2008 / Accepted: 21 February 2008 / Published: 3 March 2008
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Abstract

The electrocatalytical property of single-wall carbon nanotube (SWNT)modified electrode toward NADH detection was explored by cyclic voltammetry andamperometry techniques. The experimental results show that SWNT decrease theovervoltage required for oxidation of NADH (to 300 mV vs. Ag/AgCl) and this propertymake them suitable for dehydrogenases based biosensors. The behavior of the SWNTmodified biosensor for L-malic acid was studied as an example for dehydrogenasesbiosensor. The amperometric measurements indicate that malate dehydrogenase (MDH)can be strongly adsorbed on the surface of the SWNT-modified electrode to form anapproximate monolayer film. Enzyme immobilization in Nafion membrane can increasethe biosensor stability. A linear calibration curve was obtained for L-malic acidconcentrations between 0.2 and 1mM.
Keywords: Single wall carbon nanotubes; electrode modification; NADH; malic acid Single wall carbon nanotubes; electrode modification; NADH; malic acid
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Arvinte, A.; Rotariu, L.; Bala, C. Amperometric Low-Potential Detection of Malic Acid Using Single-Wall Carbon Nanotubes Based Electrodes. Sensors 2008, 8, 1497-1507.

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