The Synthetic Cannabinoid URB447 Exerts Antitumor and Antimetastatic Effect in Melanoma and Colon Cancer
Abstract
:1. Introduction
2. Results
2.1. Antitumor Effect of URB447 in Cancer Cell Viability
2.2. Apoptotic Effect of URB447 in Cancer Cells
2.3. Cell Cycle Interference upon URB447 Treatment
2.4. Tumor Cell Migration Is Compromised by URB447
2.5. Matrix Degrading MMP-2 and MMP-9 Secretion Is Not Altered by URB447
2.6. Daily Treatment with URB447 Reduced the Metastatic Burden in the Liver
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Cell Lines and Reagents
4.3. Cell Viability
4.4. Apoptotic Cell Determination
4.5. Cell Cycle Analysis
4.6. Wound Healing Assay
4.7. Zymography
4.8. In Vivo Liver Metastasis Assay
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Benedicto, A.; Arteta, B.; Duranti, A.; Alonso-Alconada, D. The Synthetic Cannabinoid URB447 Exerts Antitumor and Antimetastatic Effect in Melanoma and Colon Cancer. Pharmaceuticals 2022, 15, 1166. https://doi.org/10.3390/ph15101166
Benedicto A, Arteta B, Duranti A, Alonso-Alconada D. The Synthetic Cannabinoid URB447 Exerts Antitumor and Antimetastatic Effect in Melanoma and Colon Cancer. Pharmaceuticals. 2022; 15(10):1166. https://doi.org/10.3390/ph15101166
Chicago/Turabian StyleBenedicto, Aitor, Beatriz Arteta, Andrea Duranti, and Daniel Alonso-Alconada. 2022. "The Synthetic Cannabinoid URB447 Exerts Antitumor and Antimetastatic Effect in Melanoma and Colon Cancer" Pharmaceuticals 15, no. 10: 1166. https://doi.org/10.3390/ph15101166