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Mar. Drugs 2014, 12(3), 1245-1257; doi:10.3390/md12031245

Optimization of Medium Using Response Surface Methodology for Lipid Production by Scenedesmus sp.

Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
Graduate School of Chinese Academy of Sciences, Beijing 100049, China
Author to whom correspondence should be addressed.
Received: 28 November 2013 / Revised: 26 January 2014 / Accepted: 27 January 2014 / Published: 6 March 2014
(This article belongs to the Special Issue Advances and New Perspectives in Marine Biotechnology)
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Lipid production is an important indicator for assessing microalgal species for biodiesel production. In this work, the effects of medium composition on lipid production by Scenedesmus sp. were investigated using the response surface methodology. The results of a Plackett–Burman design experiment revealed that NaHCO3, NaH2PO4·2H2O and NaNO3 were three factors significantly influencing lipid production, which were further optimized by a Box–Behnken design. The optimal medium was found to contain 3.07 g L−1 NaHCO3, 15.49 mg L−1 NaH2PO4·2H2O and 803.21 mg L−1 NaNO3. Using the optimal conditions previously determined, the lipid production (304.02 mg·L−1) increased 54.64% more than that using the initial medium, which agreed well with the predicted value 309.50 mg L−1. Additionally, lipid analysis found that palmitic acid (C16:0) and oleic acid (C18:1) dominantly constituted the algal fatty acids (about 60% of the total fatty acids) and a much higher content of neutral lipid accounted for 82.32% of total lipids, which strongly proved that Scenedesmus sp. is a very promising feedstock for biodiesel production.
Keywords: optimization; lipid production; Scenedesmus sp.; response surface methodology; biodiesel optimization; lipid production; Scenedesmus sp.; response surface methodology; biodiesel
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Yang, F.; Long, L.; Sun, X.; Wu, H.; Li, T.; Xiang, W. Optimization of Medium Using Response Surface Methodology for Lipid Production by Scenedesmus sp.. Mar. Drugs 2014, 12, 1245-1257.

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