Purification and Characterization of a New Alginate Lyase from Marine Bacterium Vibrio sp. SY08
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolation and Identification of Strain SY08
2.2. Purification and Biochemical Characterization of AlySY08
2.3. Substrate Specificity and Kinetic Parameters of AlySY08
2.4. Mode of Enzyme Action and Reaction Products of AlySY08
3. Materials and Methods
3.1. Isolation and Identification of Strain SY08
3.2. Purification of AlySY08
3.3. Alginate Lyase Activity Assay
3.4. Effects of Temperature, pH, Metal Ions and Chelators
3.5. Enzymatic Kinetic Parameters Assay
3.6. Analysis of Reaction Mode and Products
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Substrate | Relative Activity (%) | Km (mg/mL) | Vmax (U/mg) |
---|---|---|---|
Sodium alginate | 100.0 ± 0.8 | 0.36 ± 0.04 | 1183.7 ± 21.5 |
PolyG blocks | 123.8 ± 2.8 | 0.34 ± 0.02 | 1255.5 ± 14.7 |
PolyM blocks | 28.2 ± 1.3 | 0.85 ± 0.16 | 512.9 ± 8.3 |
Enzyme | Source | Main Products (DP) | Ratio of Dimer (%) a | Reference |
---|---|---|---|---|
AlySY08 | Vibrio sp. SY08 | 2 | 81.4 | This study |
AlyL2-FL | Agarivorans sp. L11 | 2–3 | 64.6 | [22] |
AlyL2-CM | Agarivorans sp. L11 | 2–3 | 52.6 | [22] |
AlyL1 | Agarivorans sp. L11 | 2–3 | 47.3 | [23] |
MJ3-Arg236Ala | Sphingomonas sp. MJ-3 | 2–5 | 37.9 | [17] |
AlgMsp | Microbulbifer sp. 6532A | 2–5 | 37.5 | [26] |
AlyA1 | Zobellia galactanivorans | 2–6 | 19.0 | [24] |
Aly5 | Flammeovirga sp. MY04 | 2–7 | 15.7 | [19] |
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Li, S.; Wang, L.; Hao, J.; Xing, M.; Sun, J.; Sun, M. Purification and Characterization of a New Alginate Lyase from Marine Bacterium Vibrio sp. SY08. Mar. Drugs 2017, 15, 1. https://doi.org/10.3390/md15010001
Li S, Wang L, Hao J, Xing M, Sun J, Sun M. Purification and Characterization of a New Alginate Lyase from Marine Bacterium Vibrio sp. SY08. Marine Drugs. 2017; 15(1):1. https://doi.org/10.3390/md15010001
Chicago/Turabian StyleLi, Shangyong, Linna Wang, Jianhua Hao, Mengxin Xing, Jingjing Sun, and Mi Sun. 2017. "Purification and Characterization of a New Alginate Lyase from Marine Bacterium Vibrio sp. SY08" Marine Drugs 15, no. 1: 1. https://doi.org/10.3390/md15010001