Phytophthora austrocedri in Argentina and Co-Inhabiting Phytophthoras: Roles of Anthropogenic and Abiotic Factors in Species Distribution and Diversity
Abstract
:1. Introduction
2. Materials and Methods
2.1. Assessment of Phytophthora Diversity in Soils
2.2. Metabarcoding Analysis
2.3. Cluster Analysis of Phytophthora ß-Diversity
2.4. Assessment of the Disease Distribution over Time
3. Results
3.1. Phytophthora Diversity in Patagonian Forest Soils and P. austrocedri Distribution
3.2. Cluster Analysis of Phytophthora ß-Diversity
3.3. Disease Distribution over Time
4. Discussion
4.1. Phytophthora Diversity in Patagonian Soils
4.2. Anthropogenic Factors in Relation to PhytophthoraIinvasions and Plausible Management Strategies
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Site | Samples | Coordinates | Type of Vegetation | Geomorphology and Soil Drainage | Land Use/Anthropic Impacts | |
---|---|---|---|---|---|---|
1 | Rio Negro province, Road 40 way out El Bolsón | 49–50 | 41°53′50.3″ S, 71°30′12.4″ W | Crops, grass, and exotic ornamentals. Native forest dominated by Austrocedrus chilensis nearby. | Valley. Moderately well- to poorly-drained soils. | Cattle raising, farming, forestry, buildings. Highly anthropized area. Sampled near to the road. Austrocedrus disease in the nearby forests. |
2 | Río Negro, Golondrinas, Instituto Nacional de Tecnología Agropecuaria (INTA), Forest Reserve | 47–48 | 42°00′25.7″ S, 71°32′17.3″ W 42°00′23.4″ S, 71°32′17.4″ W | Mixed native forest dominated by A. chilensis. | Valley. Well to moderately well-drained soils. | Austrocedrus disease. Historic forestry and grazing. Nowadays is a forest reserve with recreational use. |
3 | Chubut, El Hoyo, Burned area near road 40 | 46 51–52 | 42°03′28.9″ S, 71°32′09.1″ W 42°03′00.2″ S, 71°32′28.1″ W | Burned conifer plantations and mixed native forest dominated by A. chilensis. | Valley. Well to poorly drained soils. | Grazing, exotic tree plantations. Farming nearby. Austrocedrus disease in the nearby forests. |
4 | Chubut, Route 40 at the junction of route to El Maitén | 44–45 | 42°12′28.8″ S, 71°23′00.2″ W | Mixed native forest dominated by A. chilensis. | Valley. Well to poorly drained soils. | Austrocedrus disease, road construction. |
5 | Los Alerces National Park, River Arrayanes | 21–22 | 42°44′15.5″ S, 071°44′22.8″ W | Nothofagus dombeyi dominated forests | Slopes. Well-drained soils. | Touristic and recreational use. Some grazing. |
6 | Los Alerces National Park, Braese, Road 71 | 17–20 | 42°46′23.4″ S, 71°43′50.8″ W | Mixed forest of N. dombeyi and A. chilensis | Slopes. Well-drained soils. | Touristic and recreational use. Some grazing. Austrocedrus disease. |
7 | Los Alerces National Park, Pucon Pai | 23–25 | 42°49′35.3″ S, 71°36′37.4″ W | Mixed forest dominated by A. chilensis | Slopes. Well-drained soils. | Intense touristic and recreational use. Some grazing. Austrocedrus disease. |
8 | Los Alerces National Park, cabecera | 26–27 | 42°53′00.3″ S, 71°35′44.7″ W | Mixed forest dominated by A. chilensis | Slopes. Well-drained soils. | Touristic area, one of the most visited area of the national park, some grazing, Austrocedrus disease. |
9 | Los Alerces National Park, burned area. | 13–16 | 42°53′56.21″ S, 71°37′8.08″ W | Burned area of native mixed forests dominated by A. chilensis. | Slopes. Well-drained soils. | Forest fire (10 years before), some grazing. |
10 | Chubut, Futaleufú, Huemules | 28 29–30 31–32 33 | 42°47′59.7″ S, 71°27′23.8″ W 42°48′23.3″ S, 71°27′34.8″ W 42°49′06.6″ S, 71°27′37.3″ W 42°49′33.2″ S, 71°27′29.9″ W | Native forest of Nothofagus pumilio. | Slopes. Well-drained soils. | Cattle raising and forestry. |
11 | Los Alerces National Park, ranch Burgos | 53–54 | 43°9′38.00″ S, 71°42′54.00″ W 43°9′46.26″ S, 71°41′38.99″ W | A. chilensis dominated forests. | Valley. Moderately well- to poorly- drained soils. | Cattle raising and forestry (area with private use inside the national park). Austrocedrus disease. |
12 | Chubut, Trevelin, Ranch “La 106” | 1–6 | 43°11′59.7″ S, 71°33′30.1″ W | Austrocedrus chilensis dominated forests. | Plains and hill slopes. Moderately well- to very poorly-drained soils. | Cattle raising, forestry and forest plantations, Austrocedrus disease. |
13 | Reserve Nant y Falls cascades | 7–12 | 43°11′28″ S, 71°28′11″ W | Austrocedrus chilensis dominated forests. | Plains and hill slopes. Moderately well- to very poorly-drained soils. | Austrocedrus disease, cattle raising and historic forestry. Nowadays forests are protected. |
14 | Corcovado Ruta 17 | 40–41 42–43 | 43°31′32.3″ S, 71°32′30.8″ W 43°31′37.7″ S, 71°32′32.1″ W | Austrocedrus chilensis dominated forests with areas of mallín (wet land). | Valley. Somewhat poorly- drained to poorly- drained soils. | Cattle raising, some forestry, and Austrocedrus disease. |
15 | Chubut, Corcovado, Santa Teresita Ranch | 34 35 36–37 38–39 | 43°32′05.2″ S, 71°33′04.5″ W 43°32′00.1″ S, 71°32′28.1″ W 43°32′01.9″ S, 71°32′30.7″ W 43°31′55.5″ S, 71°32′30.5″ W | Austrocedrus chilensis dominated forests | Valley. Moderately well- to poorly-drained soils. | Cattle raising, Austrocedrus disease |
Species | Clade | Site 1 | Site 2 | Site 3 | Site 4 | Site 6 | Site 7 | Site 8 | Site 9 | Site 10 | Site 11 | Site 12 | Site 13 | Site 14 | Site 15 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Phytophthora aleatoria P.M. Scott, R. L McDougal & P.M Taylor /cactorum(Lebert & Cohn) J. Schröt. | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 411 | 0 | 0 | 0 | 271 |
P. idaei D.M. Kenn | 1 | 92 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
P. capensis Bezuid., Denman, A. McLeod & S.A. Kirk /citricola Sawada | 2 | 1219 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
P. caryae N.J. Brazee, X. Yang & C.X. Hong /pini Leonian | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 76 | 0 | 0 | 0 | 1889 | 536 |
P. plurivora T. Jung & T.I. Burgess | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 84 |
P. ilicis Buddenh. & Roy A. Young | 3 | 0 | 0 | 162 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 198 | 0 | 287 | 4511 |
P. pseudosyringae T. Jung & Delatour | 3 | 858 | 150 | 0 | 0 | 0 | 0 | 0 | 0 | 492 | 1632 | 0 | 0 | 0 | 127 |
P. bilorbang Aghighi, G.E. Hardy, J.K. Scott & T.I. Burgess | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 196 | 0 |
P. chlamydospora Brasier & E.M. Hansen | 6 | 667 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 181 | 0 | 0 | 771 | 929 | 0 |
P. gonapodyides (H.E. Petersen) Buisman | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 222 | 0 | 88 | 239 |
P. megasperma Drechsler | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 581 | 0 | 0 | 0 | 524 | 0 |
P. mississippiae Xiao Yang, Copes & C.X. Hong | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 100 | 0 | 0 | 0 | 0 | 0 |
P. × cambivora (Petri) Buisman | 7 | 1687 | 776 | 2356 | 1169 | 3823 | 1896 | 1088 | 5337 | 1836 | 0 | 6450 | 4629 | 644 | 2453 |
P. cinnamomi Rands | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 709 | 0 | 215 | 0 | 0 |
P. austrocedri Gresl. & E. M. Hansen | 8 | 4517 | 3798 | 7143 | 3525 | 8398 | 0 | 3297 | 0 | 8268 | 10,375 | 6371 | 12,583 | 7118 | 17,447 |
P. cryptogea Pethybr. & Laff./pseudocryptogea Safaiefarahani, Mostowfizadeh, Hardy & Burgess | 8 | 822 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
P. lateralis Tucker & Milbrath | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 393 | 0 | 0 | 0 | 0 | 279 | 0 |
P. obscura Grünwald & Werres | 8 | 324 | 0 | 89 | 0 | 336 | 0 | 311 | 0 | 1859 | 0 | 701 | 1242 | 1504 | 458 |
P. primulae J.A. Toml. | 8 | 1505 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
P. pseudocryptogea Safaiefarahani, Mostowfizadeh, Hardy & Burgess | 8 | 1305 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
P. ramorum Werres, De Cock & Man | 8 | 2673 | 3752 | 692 | 527 | 0 | 0 | 962 | 0 | 5459 | 455 | 159 | 57 | 349 | 1408 |
P. syringae (Kleb.) Kleb. | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1952 | 0 | 343 | 0 | 600 | 0 |
P. kernoviae Brasier, Beales & S.A. Kirk | 10 | 236 | 0 | 0 | 0 | 0 | 5450 | 0 | 474 | 2853 | 0 | 1818 | 1492 | 187 | 2605 |
Unknown oomycete genus | 1603 | 594 | 55 | 0 | 2537 | 2442 | 0 | 451 | 3821 | 457 | 2043 | 3199 | 7827 | 3567 |
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Vélez, M.L.; La Manna, L.; Tarabini, M.; Gomez, F.; Elliott, M.; Hedley, P.E.; Cock, P.; Greslebin, A. Phytophthora austrocedri in Argentina and Co-Inhabiting Phytophthoras: Roles of Anthropogenic and Abiotic Factors in Species Distribution and Diversity. Forests 2020, 11, 1223. https://doi.org/10.3390/f11111223
Vélez ML, La Manna L, Tarabini M, Gomez F, Elliott M, Hedley PE, Cock P, Greslebin A. Phytophthora austrocedri in Argentina and Co-Inhabiting Phytophthoras: Roles of Anthropogenic and Abiotic Factors in Species Distribution and Diversity. Forests. 2020; 11(11):1223. https://doi.org/10.3390/f11111223
Chicago/Turabian StyleVélez, María Laura, Ludmila La Manna, Manuela Tarabini, Federico Gomez, Matt Elliott, Pete E. Hedley, Peter Cock, and Alina Greslebin. 2020. "Phytophthora austrocedri in Argentina and Co-Inhabiting Phytophthoras: Roles of Anthropogenic and Abiotic Factors in Species Distribution and Diversity" Forests 11, no. 11: 1223. https://doi.org/10.3390/f11111223