Next Article in Journal
Posttranslational Modifications of HIV-1 Integrase by Various Cellular Proteins during Viral Replication
Previous Article in Journal / Special Issue
Role of Polycomb Proteins in Regulating HSV-1 Latency
Article Menu

Export Article

Open AccessReview
Viruses 2013, 5(7), 1758-1786; doi:10.3390/v5071758

Chromatin Dynamics during Lytic Infection with Herpes Simplex Virus 1

Department of Biochemistry, Li Ka Shing Institute of Virology, University of Alberta, Edmonton, AB T6G 2H7, Canada
Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, AB T6G 2E1, Canada
Authors to whom correspondence should be addressed.
Received: 13 June 2013 / Revised: 6 July 2013 / Accepted: 8 July 2013 / Published: 16 July 2013
(This article belongs to the Special Issue Chromatin Control of Viral Infection)
View Full-Text   |   Download PDF [2779 KB, uploaded 12 May 2015]   |  


Latent HSV-1 genomes are chromatinized with silencing marks. Since 2004, however, there has been an apparent inconsistency in the studies of the chromatinization of the HSV-1 genomes in lytically infected cells. Nuclease protection and chromatin immunoprecipitation assays suggested that the genomes were not regularly chromatinized, having only low histone occupancy. However, the chromatin modifications associated with transcribed and non-transcribed HSV-1 genes were those associated with active or repressed transcription, respectively. Moreover, the three critical HSV-1 transcriptional activators all had the capability to induce chromatin remodelling, and interacted with critical chromatin modifying enzymes. Depletion or overexpression of some, but not all, chromatin modifying proteins affected HSV-1 transcription, but often in unexpected manners. Since 2010, it has become clear that both cellular and HSV-1 chromatins are highly dynamic in infected cells. These dynamics reconcile the weak interactions between HSV-1 genomes and chromatin proteins, detected by nuclease protection and chromatin immunoprecipitation, with the proposed regulation of HSV-1 gene expression by chromatin, supported by the marks in the chromatin in the viral genomes and the abilities of the HSV-1 transcription activators to modulate chromatin. It also explains the sometimes unexpected results of interventions to modulate chromatin remodelling activities in infected cells. View Full-Text
Keywords: Herpes simplex virus 1; chromatin; silencing; nucleosome; histone; fluorescence recovery after photobleaching (FRAP) Herpes simplex virus 1; chromatin; silencing; nucleosome; histone; fluorescence recovery after photobleaching (FRAP)

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Conn, K.L.; Schang, L.M. Chromatin Dynamics during Lytic Infection with Herpes Simplex Virus 1. Viruses 2013, 5, 1758-1786.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top