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Article

Antagonism and Synergism Characterize the Interactions between Four North American Potato Virus Y Strains

by
Prakash M. Niraula
1,
Patricia Baldrich
2,
Junaid A. Cheema
1,
Hashir A. Cheema
1,
Dejah S. Gaiter
1,
Blake C. Meyers
2 and
Vincent N. Fondong
1,*
1
Department of Biological Sciences, Delaware State University, Dover, DE 19901, USA
2
Donald Danforth Plant Science Center, St. Louis, MO 63132, USA
*
Author to whom correspondence should be addressed.
Int. J. Plant Biol. 2024, 15(2), 412-428; https://doi.org/10.3390/ijpb15020032
Submission received: 5 April 2024 / Revised: 8 May 2024 / Accepted: 16 May 2024 / Published: 21 May 2024
(This article belongs to the Topic Microbe-Induced Abiotic Stress Alleviation in Plants)
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Abstract

:
Potato virus Y (PVY) is one of the most important constraints to potato production worldwide. There is an increasing occurrence of recombinant PVY strains PVYNTN and PVYN-Wi and a decline in the incidence of the nonrecombinant PVYO. We hypothesized that this may be due to the ability of these recombinant strains to antagonize and/or outcompete PVYO in mixed infections. To determine this, we investigated interactions between PVYO and three recombinant PVY strains common in North America: PVYNTN, PVYN-Wi, and PVYN:O. Overall, our study showed that these interactions are tissue-dependent. Specifically, PVYNTN, the main causal agent of potato tuber necrotic ringspot disease (PTNRD), was found to be more adaptable than PVYO, especially in potato leaves due, at least in part, to the Ny gene that confers hypersensitive resistance (HR) to PVYO. Furthermore, PVYN-Wi was found to repress PVYO in potato tubers but act synergistically in potato leaves. The PVYO-induced foliage necrosis in cultivar ‘Ranger Russet’ was observed to be more severe in plants co-infected by PVYN-Wi and PVYN:O, respectively, resulting in plant death. Strikingly, this PVYO -induced necrosis was suppressed by PVYNTN in doubly infected plants. These interactions may, at least partially, explain the decreasing incidence of PVYO in United States potato production regions, especially given that many cultivars contain the Ny gene, which likely limits PVYO enabling PVYNTN and PVYN-Wi to outcompete. We also found that replication and cell-to-cell movement of these PVY strains in tubers at 4 °C was similar to levels at ambient temperature.

1. Introduction

Potato virus Y (PVY) is one of the most important pathogens of solanaceous plants, such as potato, tobacco, pepper, and ornamental plants, leading to significant losses of the crop [1]. In potato, diseases caused by PVY pose critical challenges to the production of the crop worldwide. PVY is disseminated in potato by aphids and through seed tubers, which are the main mode of potato propagation. The virus exists as a complex of strains that induce a wide variety of foliar and tuber symptoms in potato, leading to yield reductions and loss of tuber quality. PVY evolves through accumulation of mutations and rapidly through recombination between different strains, adapting to new potato cultivars across different environments [2]. Although many PVY recombinants with important genome differences have been identified, almost all these recombinants have PVYO and PVYN as parents [3,4]. In recent decades, new recombinant strains have emerged that have rapidly adapted to the potato ecosystem; these strains continue to dominate virus populations over vast geographical areas in North America [3,5,6]. These recombinants tend to cause milder foliar symptoms; thus, infected plants are less apparent than parent strains, but they cause more severe agricultural losses due to tuber necrosis, which reduces tuber quality. Usual foliar symptoms caused by PVY include leaf mosaic, crinkling, localized necrotic lesions, and leaf drop. Some PVY strains induce distinct ring patterns on the surface of tubers, causing the so-called potato tuber necrotic ringspot disease (PTNRD), which is one of the most damaging viral diseases in potatoes and poses a serious threat to seed and commercial potato production industries [2].
To date, at least nine recombination patterns of PVYO and PVYN sequences have been identified in potato-infecting PVY isolates; the three most common recombinant patterns characteristic of PVY strains are PVYNTN, PVYN:O, and PVYN-Wi [7,8]. PVYN:O and PVYN-Wi are believed to have acquired their recombinant PVYO segments from two separate PVYO lineages, while PVYNTN, which has risen globally and overpopulated other strains worldwide, is thought to have acquired its recombinant PVYO segment from the same lineage as PVYN:O [4].
Recently, the incidence and occurrence of the strain PVYNTN have been increasing in the United States seed potato crops, while PVYO, the ordinary, non-recombinant strain, has been decreasing [9]. PVYNTN is the main cause of PTNRD in susceptible potato cultivars, leading to reductions in tuber quality and quantity [10]. PVYN-Wi has also been reported to cause PTNRD in the United States and Canada [3]. Interestingly, PVYNTN and PVYN-Wi infections are usually either asymptomatic to the foliage or result in transient mild mosaic patterning [2]. On the other hand, PVYO typically causes a strong hypersensitive resistance (HR), including localized necrosis and leaf drop and/or mild mosaic symptoms on the foliage of potato cultivars having the Ny resistance gene. PVYO-induced HR is mediated by the Ny gene, which confers partial resistance in many North American potato varieties [8]. PVYN:O is less damaging than other strains, but causes mosaic symptoms in susceptible potato varieties [11].
Interactions between PVY strains and potato are defined in large part by resistance genes. Two types of PVY single dominant resistance genes have been identified in potato, namely, R genes that confer extreme resistance (ER) and N genes that confer HR [12]. Only a limited number of cultivated potatoes contain R genes, including Rysto from S. stoloniferum [13]. Unlike R genes, N genes occur widely in cultivated potato, and the ability of potatoes containing N genes to exhibit the HR upon PVY infection is strain-specific [14,15]. Thus, Nctbr and Ncspl from S. tuberosum and S. sparsipilum, respectively, confer HR to PVYC only, and the Nytbr from S. tuberosum confers HR resistance to PVYO isolates only. Strain groups PVYC, PVYO, and PVYZ elicit HR phenotypes Nc, Ny, and Nz, respectively, while PVYD elicits HR phenotypes Ny an Nc [16]. In addition to PVYO, the strains PVYC, PVYD, PVYN, and PVYZ are non-recombinant and serve as parents for many recombinant strains. PVYZ was first described based on its ability to elicit HR in a potato cultivar carrying the Nz gene and the inability to induce necrosis in tobacco [17].
There are growing reports that PVYNTN has been spreading rapidly in North America, and that the occurrence of PVYO, the ordinary strain, has been decreasing [2,3,6,9,15]. We therefore hypothesized that antagonistic interactions between PVYO and other PVY strains have led to a decline in importance of the former. In this study, we assessed the infectivity of PVYO, PVYNTN, PVYN:O, and PVYN-Wi in N. tabacum and in tubers of potato cultivars ‘Desiree’, ‘Ranger Russet’, ‘Russet Norkotah’ and ‘Eva’, which have different levels of resistance to PVY. Our results indicate a high adaptability of PVYNTN and that it outcompetes PVYO, especially in leaves. Furthermore, PVYN-Wi was shown to antagonize PVYO but act synergistically with PVYNTN and PVYN:O. These findings may, at least partially, explain the current PVY infection dynamics in North America.

2. Materials and Methods

2.1. Virus Inoculations

Virus inoculum was prepared by homogenizing systemically infected leaves of N. tabacum plants in a 0.01 M phosphate buffer, pH 7.0, at a dilution of 1/20 (w/v). Control plants were inoculated with sap from PVY-free N. tabacum cv. Samsun plants. Plants were placed in a growth room at 16 h light and 22 ± 3 °C temperature. At five days post-infection (dpi), whole inoculated leaves were collected for each of three biological replications, ground to powder in liquid nitrogen, and then stored at −80 °C until RNA extraction. Symptoms produced by each strain were recorded for up to 30 dpi.
The four PVY strains from the United States (kindly provided by Stewart Gray of Cornell University) investigated in this study were collected between 2004 and 2006. Three of the strains, PVYO (isolate NY090031; GenBank # KY848009), PVYNTN (isolate NY090029; GenBank # KY848008), and PVYN:O (isolate NY090004; GenBank # KY848007), were isolates from New York State (PVYO, PVYNTN, and PVYN:O), while the fourth strain, PVYN-Wi (isolate MN15_G_52; GenBank # KY847981), was from the state of Minnesota [5]. These isolates were confirmed by RT-PCR of total RNA from tuber samples using strain-specific primers listed in Table 1. Virus inoculum was then prepared by homogenizing systemically infected leaves (at 14 to 21 dpi) of N. tabacum plants in a 0.01 M phosphate buffer, pH 7.0, at a dilution of 1/20 (w/v). The control was sap from PVY-free N. tabacum plants. PVY strain infectivity was investigated in potato foliage using the cultivar ‘Ranger Russet’. The inoculum was prepared as described above. Plants were inoculated at a 4 to 5 leaf stage. Inoculated plants were placed in a room at 16 h light and 22 ± 3 °C temperature.
To characterize virus replication and cell-to-cell movement in potato tubers, we investigated three potato cultivars with different levels of resistance to PVY, namely, ‘Desiree’ (susceptible), ‘Ranger Russet’ (moderately susceptible) [3], and ‘Eva’ (extreme resistance). All tubers used in these experiments were at the sprouting stage and were tested with reverse transcription polymerase chain reaction (RT-PCR) and confirmed to be PVY-free. To inoculate the tubers, a 1 cm corkborer was used to produce a well in the tuber pith (inner medulla) at the center of approximately 2 cm thick sections of potato tubers. Corkborer wells were sealed at one end of the tuber section with an agarose gel in a Petri dish. Virus inoculum was prepared by homogenizing systemically infected leaves of N. tabacum plants in a tuber inoculation buffer (20 mM PBS, 2% PVFP, 20 mM Na2SO3, 2 mM EDTA) at a dilution of 1/20 (w/v), followed by centrifugation at 10,000 revolutions per minute (rpm) for 5 min. Approximately 150 μL of supernatant was placed in the well, and the inoculated tubers were placed in a closed box and incubated until sample collection, which was conducted using a 0.5 cm corkborer. Samples were taken in triplicate, frozen in liquid nitrogen, and stored at −80 °C until RNA extraction.

2.2. Isolation of RNA and Reverse Transcription Quantitative PCR (RT-qPCR)

Total RNA was isolated from tuber and leaf samples using the RNeasy Mini kit (Qiagen, Germantown, MD, USA) with modifications. Here, approximately 200 mg of ground leaf and tuber tissues, respectively, were aliquoted for total RNA extraction using a leaf extraction buffer (5 M guanidine thiocyanate, 50 mM Tris-HCl pH 8, 10 mM EDTA, 2% N-lauroylsarcosine) and a tuber RNA extraction buffer (6 M guanidine hydrochloride, 20 mM MES hydrate, 20 mM EDTA, pH 8, 10% β-mercaptoethanol). The quality and quantity of RNA were determined using a NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and agarose gel electrophoresis. The Invitrogen SuperScript III Platinum™ SYBR™ green one-step RT-qPCR Kit (Thermo Fisher Scientific, USA) was used to quantify viral RNA in approximately 50 ng of total RNA on a Light Cycler 480 system (Roche Diagnostics, Indianapolis, IN, USA). The 10 µL reaction mix contained 5 µL SYBR Green supermix, 0.25 µL SuperScript III reverse transcriptase (RT)/Platinum® Taq DNA polymerase enzyme mix, 0.4 µL 100 µM forward and reverse primers, and 2 µL of total RNA. The RT-qPCR conditions were as follows: cDNA synthesis at 50 °C for 15 min; qPCR cycling at 95 °C for 5 min and 45 three-step cycles, each at 95 °C for 15 s, 60 °C for 30 s, and 72 °C for 10 s, followed by a final melting curve of 65–95 °C for 5 s. The specificities of primers used in RT-qPCR analyses were determined by running amplification products in an agarose gel and confirming amplification of a single band corresponding to the expected size. Furthermore, amplification efficiencies were determined in a RT-qPCR assay using duplicates of a 10-fold dilution series (four dilutions) as the template.
Mean threshold cycle (Ct) values of the dilution series were plotted against log (1/dilution factor), and the resulting slope value was used to calculate the amplification efficiency (E) using the equation: E = 10−1/slope (Rasmussen 2001). Primer pairs had efficiencies ranging from 95 to 109% (Table 1; Supplementary Figure S1), which were, therefore, within the recommended range [20].

2.3. Determination of Relative Viral RNA Titer

Here, we used elongation factor 1 (EF1) as the housekeeping gene. To compensate for potential variation between runs and plates, EF1 Ct values were normalized by subtracting the median Ct value from individual EF1 Ct values in each replicate. Normalized EF1 Ct values were then subtracted from individual target Ct values to obtain normalized target Ct values, which were used to calculate target ΔCt and ΔΔCt values. In the calculation of the relative viral RNA titer, we removed variation from background signals unrelated to viral RNA target by subjecting healthy tissues to the same amplification conditions as for each treatment [21]; this was followed by calculation of the mean ΔCt and ΔΔCt of the background noise. These background ΔCt and ΔΔCt values were then subtracted from experimental ΔCt and ΔΔCt values, respectively, the results of which were used to calculate individual 2−ΔΔCt values [22] to obtain relative viral RNA levels. Error bars were computed to indicate the standard deviation of three different technical repeats from three biological replicates, and significant differences were determined using a t-test.

3. Results

3.1. Antagonistic and Synergistic Interactions between PVY Strains in Potato

There are several reports that PVYNTN has been spreading rapidly in North America, and that the occurrence of PVYO, the ordinary strain, has been decreasing [3,6,15,18]. We investigated whether this may be due to a PVYNTN competitive advantage over PVYO, during interactions between these viral strains in co-infected plants. We therefore examined co-infections in the potato cultivar ‘Ranger Russet’, which displays an HR upon PVYO infection, due to the presence of the Ny gene [23]. The results showed that leaves of ‘Ranger Russet’ inoculated with PVYO displayed severe local veinal necrosis within 5 dpi in inoculated leaves, and by 10 dpi, these symptoms had spread to systemically infected leaves, which displayed yellowing (Figure 1A). By 21 dpi, severe veinal and systemic necrosis had spread to all leaves of plants inoculated with PVYO (Supplementary Figure S2A). This PVYO-induced systemic necrosis, which is triggered by the corresponding N gene, resulted in extensive leaf-drop within 30 dpi (Figure 1B). We note that although the Hc-Pro avirulence factor of PVYO restricts virus spread from inoculated leaves of potato cultivars with the Ny gene [23], it has been observed that this resistance is usually overcome, due to other host factors and/or environmental conditions [24,25,26,27,28], resulting in severe systemic necrosis and leaf-drop [15]. As discussed later in this text, these differential symptoms between PVYO, PVYNTN, and PVYN-Wi have been shown to be due to Hc-Pro, which has been identified as the PVY avirulence factor that corresponds to Ny tbr and Nc spl [29].
We then investigated whether phenotypes displayed by singly infected plants could be influenced by other PVY strains by doubly inoculating 5 to 6 plants and comparing them to symptoms displayed by single infections. Interestingly, in all inoculated plants, the systemic necrosis caused by PVYO was more severe when the latter was co-inoculated with PVYN-Wi and PVYN:O, and leaves of these plants displayed severe necrosis and leaf yellowing by 10 dpi (Figure 1A). These symptoms became severe and resulted in plant death within 30 dpi (Figure 1B; Supplementary Figure S2B). Plant death started from the lower stem and progressed to the apical meristem, which, although wilted, had not died at 30 dpi, when observations were discontinued. Also, plants inoculated with PVYN-Wi and PVYN:O displayed systemically infected leaves with a deep orange phenotype (Supplementary Figure S2A). Strikingly, the systemic necrosis and leaf drop caused by PVYO was observed to be absent in plants doubly infected with PVYNTN and PVYO. These plants displayed a phenotype similar to the phenotype displayed by plants singly infected with PVYNTN. Thus, the ability of PVYO to cause systemic necrosis and leaf drop in ‘Ranger Russet’ is repressed by PVYNTN (Figure 1B). Together, these symptoms show that PVYO interacts synergistically with PVYN-Wi and PVYN:O but is antagonized by PVYNTN in ‘Ranger Russet’.

3.2. RT-qPCR Quantification of Viral RNA

To determine the effect of mixed infections on viral RNA levels, viral RNA was quantified in singly and doubly infected plants at 7 dpi from inoculated leaves and at 30 dpi from newly emerged leaves. RT-qPCR quantification of viral RNA was carried out using strain-specific primers. The specificity of the primers used in this study was confirmed by running RT-PCR amplification products on an agarose gel (Supplementary Figure S3). All experiments had three biological replicates and three technical replicates. The results showed that at 7 dpi (Figure 2A(a–d)), there were almost undetectable levels of PVYO, likely due to the Ny gene in ‘Ranger Russet’ that confers HR, characterized by necrosis to PVYO. Interestingly, in the presence of PVYN-Wi, there was a significant increase in the level PVYO RNA. Interactions between PVYN:O and PVYN-Wi, as well as between PVYN:O and PVYO, which resulted in significantly increased levels of PVYN:O. These increases in viral RNA levels indicate synergism between PVYN:O and PVYN-Wi, as well as between PVYN:O and PVYO. The very high RNA levels in plants inoculated with PVYN-Wi and PVYO, and with PVYN:O and PVYN-Wi may at least partly explain plant death in these plants. There were almost undetectable levels of viral RNA in samples collected from the apical meristem of plants inoculated with PVYNTN at 30 dpi (Figure 2A(e–h)), consistent with the apparent absence of symptoms in these plants. This is likely due to a deficiency in long-distance movement of this strain. We note that although, by 30 dpi, most of the stem of all plants inoculated with PVYN:O and PVYO, as well as PVYN-Wi and PVYO, had died, the apical part of each plant was still in a condition (Figure 1B) for samples to be collected for RNA analysis.
Because ‘Ranger Russet’ has the Ny gene, which confers HR to PVYO, we reasoned that the presence of this gene may have influenced interactions between PVYO and other PVY strains. Thus, we further investigated these interactions in ‘Russet Norkotah’, which does not contain the Ny gene [8]. Samples were collected for viral RNA quantification at 7 and 30 dpi from inoculated and systemic leaves, respectively. Analysis of viral RNA from three biological replicates and three technical replicates at 7 dpi showed that, as is consistent with results recorded in ‘Ranger Russet’, PVYO levels were significantly lower in ‘Russet Norkotah’ leaves when co-inoculated with PVYNTN, PVYN-Wi, and PVYN:O, respectively (Figure 2B(i)). Similar results were recorded at 30 dpi (Figure 2B(m)). Strikingly, unlike the result obtained for ‘Ranger Russet’, PVYNTN levels were significantly lower in mixed infections with PVYO (Figure 2B(j,n)), indicating that the presence of the Ny gene likely inhibited the ability of PVYO to compete with PVYNTN in ‘Ranger Russet’. Curiously, PVYN-Wi showed very low levels in systemically infected leaves of ‘Russet Norkotah’, except in the presence of PVYNTN at 30 dpi (Figure 2B(o)). Together, these results show differential influences on virus replication and movement in the presence of the Ny gene.

3.3. Replication and Cell-To-Cell Movement of PVY Strains

Potato-infecting viruses, as well as viruses of other tuberous root crops, tend to spend more time in tubers than elsewhere in the plant. Thus, here, we investigated the ability of all four PVY strains to replicate and move in tubers using tubers of three potato cultivars with different levels of resistance to PVY. Two of the cultivars, ‘Desiree’ and ‘Ranger Russet’, contained N genes [23], while the third cultivar, ‘Eva’, contained the Ryadg extreme resistance gene from S. tuberosum subsp. andigena [30]. A key limitation in analyzing potato tuber RNA is the difficulty of extracting good-quality total RNA using RNA extraction buffers available in conventional and commercial kits. This is because potato tuber starch is highly phosphorylated and the negatively charged phosphate groups repel adjacent starch chains, which facilitates hydration and gelling [31,32,33,34]. This causes the starch to swell into a viscous gel in the presence of the guanidine thiocyanate found in standard RNA extraction kits, thereby making recovery of a supernatant and RNA isolation problematic. We therefore developed a tuber RNA extraction buffer containing guanidine hydrochloride and MES hydrate, which gave the very high-quality tuber RNA analyzed in this study. A typical ethidium bromide-stained agarose electrophoresis gel of total tuber RNA is shown in Supplementary Figure S4.
To determine the tuber infectivity of these PVY strains, we inoculated tubers with sap from N. tabacum infected by each of the four PVY strains at a dilution of 1/20 (w/v), as described in Materials and Methods. Each treatment had three biological replicates, as well as three technical replicates. Inoculated tubers were stored at 22 °C. At 6 h post-inoculation (hpi), samples were collected at 1 cm from the inoculum well using a 0.5 cm corkborer. Total tuber RNA was extracted, and viral RNA was quantified with RT-qPCR using universal PVY primers. The results showed that PVY strain infectivity was influenced by the potato genotype (Figure 3). As expected, tubers of ‘Eva’, which contains the PVY extreme resistance gene, displayed the lowest amount of viral RNA. The level of PVYNTN RNA was significantly higher than that of other strains, except in ‘Desiree’, in which there was no significant difference between PVYNTN and PVYN-Wi levels (Figure 3). Overall, PVYO displayed the lowest amount of viral RNA, due at least in part to the presence in these cultivars of the Ny gene, which confers a HR reaction to PVYO infection. Further, except for ‘Eva’, PVYN-Wi displayed significantly higher levels of viral RNA than those inoculated with PVYN:O. These results confirm that the extreme resistance in ‘Eva’ [30] is not limited to aphid transmission through leaves, but also includes replication and cell-to-cell movement in tubers. The low levels of viral RNA in ‘Eva’ compared with levels in the less resistant ‘Ranger Russet’ and ‘Desiree’ show that the corkborer method can be used to quantify PVY replication in tubers.

3.4. Interactions between PVY Strains in Potato Tubers

There is evidence that the nature of interaction between PVY strains is dependent on the type of tissues co-infected [35]. To determine whether the apparent antagonism exhibited by PVYNTN over PVYO observed in potato foliage occurs in potato tubers, we investigated the effect of mixed infection between these two PVY strains in tubers of ‘Ranger Russet’. Thus, tubers were singly and doubly inoculated with sap from N. tabacum leaves infected by both strains and samples collected at 6, 24, 48, and 96 h post-infection (hpi). To determine the effect of mixed infection on virus replication and cell-to-cell movement at these time points, samples were collected along the infection gradient (from positions “a” to “c”) as illustrated in Figure 4A. Using strain-specific primers, we carried out an RT-qPCR analysis of PVYNTN and PVYO in mixed and singly infected tubers. Within 6 hpi, viral RNA was detected at position “a” (1 cm) but not at position “b”, indicating that the virus was still within a 1 cm radius of the site of inoculation. Interestingly, levels of PVYNTN RNA were lower in mixed infections than in singly inoculated tubers, especially from 48 to 96 hpi (Figure 4B). This result suggests antagonism between the two PVY strains in potato tubers. The extent of PVYNTN antagonism on PVYO declined over distance with a decline in PVYNTN levels (Figure 4C), suggesting that the ability of PVYNTN to suppress PVYO is concentration-dependent.
We further investigated the interaction of other PVY strains in tubers of ‘Ranger Russet’. Thus, tubers were doubly inoculated with pairs of all four strains and viral RNA levels compared between single and co-infections using strain-specific as well as universal primers. In ‘Ranger Russet’ tubers, the results indicated that in tubers, mixed infection between PVYN:O and PVYO appeared not to significantly affect levels of either strain (Figure 5A). Correspondingly, PVYN-Wi levels appeared not to be affected by PVYO, which itself showed a significant decrease when co-infected with PVYN-Wi (Figure 5B). Also, mixed infection between PVYN:O and PVYNTN caused no significant effect on PVYNTN, but resulted in an increase in the level of PVYN:O (Figure 5C). Additionally, PVYN-Wi and PVYNTN antagonized each other (Figure 5D). Moreover, except for tubers doubly infected with PVYN-Wi and PVYNTN, all doubly infected tubers recorded higher viral RNA loads than singly infected tubers, as determined with universal primers (Supplementary Figure S5).
To determine whether interactions between PVY strains were influenced by the Ny gene, we investigated these interactions in tubers of ‘Russet Norkotah’, which does not contain the gene. The results showed that PVYN-Wi and PVYNTN each acted synergistically with PVYO [35,36]. Strikingly, unlike observations in ‘Ranger Russet’, in ‘Russet Norkotah’, there were significantly lower levels of PVYNTN in leaves co-inoculated with PVYO. (Supplementary Figure S6). Thus, PVYO likely represses PVYNTN in the absence of the Ny gene, to which the host exhibits a HR in the presence of PVYO. Levels of PVYN:O were also repressed by PVYNTN and PVYN-Wi, and not by PVYO in ‘Russet Norkotah’ leaves, but showed a significant increase in tubers co-infected with PVYNTN.

3.5. Effect of Temperature on PVY Cell-to-Cell Movement in Tubers

To determine whether low seed storage temperature influences PVY replication and cell-to-cell movement in potato tubers, we quantified the levels of PVYO and PVYNTN RNA over time in tubers at 4 °C and at 22 °C, respectively. Samples were collected at 6, 24, and 48 hpi using a 0.5 cm corkborer at an approximate distance of 5 mm from the inoculation well, at position “a”, as well as at positions “b” and “c”, for a total distance of approximately 1.5 cm from the center of the well, as illustrated in Figure 4A. The results of RT-qPCR quantification indicated that under both temperature conditions, viral RNA was detected at position “a” at 6 hpi, thus indicating that both PVY strains were replicating and carrying out cell-to-cell movement. Interestingly, viral RNA levels declined in samples collected from the same position over time, which may be due to the tuber’s antiviral response.
There were differences between the levels of viral RNA in the two strains. For instance, PVYNTN RNA was significantly higher in ‘Desiree’ tubers at 4 °C compared with 22 °C from positions “a” through “c”. No decline was observed in ‘Ranger Russet’ at position “c” (Figure 6A). In ‘Eva’, there was almost no detectable viral RNA beyond position “a”, consistent with the fact that ‘Eva’ has PVY extreme resistance, which likely involves virus movement. This result contrasts with the high levels of viral RNA observed along the infection gradient of tubers of the more susceptible ‘Desiree’ and ‘Ranger Russet’. Once again, the almost indetectable levels of viral RNA in ‘Eva’, compared with the less resistant cultivars is further evidence of viral replication and validates use of this approach to quantify virus replication in tubers. Consistent with results obtained in infectivity assessment above, we observed lower levels of PVYO in tubers of all three cultivars at both incubation temperatures compared with levels of PVYNTN, especially at 22 °C, where ‘Eva’ and ‘Ranger Russet’ showed very low levels of PVYO RNA (Figure 6B). PVYO RNA levels were more uniform in ‘Desiree’ tubers at 22 °C.

3.6. Evidence of Virus Replication in Potato Tubers

To confirm that the viral RNA detected in this study was from a replicating virus, we carried out an RT-PCR targeting each of PVYNTN and PVYO Hc-Pro cistron negative sense strand RNA [(−)ssRNA], which, unlike the (+)ssRNA, is not packaged in the virion. Here, samples were collected at 6, 24, 48, and 96 hpi, and total tuber RNA was extracted as described in Materials and Methods for reverse transcription of the (−)ssRNA of the Hc-Pro cistron. Reverse transcription was carried out using the Verso 1-step RT- PCR Reddymix kit (Thermo Fisher Scientific, Waltham, MA USA) at 50 °C for 20 min with a primer targeting the (−)ssRNA of the Hc-Pro cistron. The reverse transcriptase was then inactivated for 10 min at 95 °C prior to PCR amplification of the cDNA. Two microliters of the reverse transcription reaction were utilized for PCR amplification using the Dreamtaq PCR mastermix kit (Thermo Fisher Scientific, Waltham, MA USA) and both Hc-Pro forward and reverse primers (Table 1). Agarose gel analysis of the PCR products showed the presence of an amplicon corresponding to Hc-Pro cistron (Supplementary Figure S7). Given that the inoculum used in this study was crude sap, where viral RNA is naturally degraded by RNAases, the (−)ssRNA obtained in this analysis could only have come from a replicating virus. This result, together with low levels of viral RNA recorded in cultivar ‘Eva’ as compared to RNA levels in the less resistant ‘Ranger Russet” and ‘Desiree’ observed above, indicates that the viral RNA detected in tubers in this study was from a replicating virus. Thus, the corkborer approach can be used to quantify replicating viral RNA in tubers.

4. Discussion

This study showed that the levels of PVYNTN RNA were consistently higher in potato tubers than those of PVYO. The results also showed that during co-infection, PVYNTN outcompeted PVYO in tubers of ‘Ranger Russet’ (Figure 6B) but not in those of ‘Russet Norkotah’. This was likely due to the presence in ‘Ranger Russet’ of Ny gene, which confers resistance to PVYO, but not to PVYNTN. Given the increasing importance of potato cultivars with the Ny gene in commercial potatoes [8,23,37], these interactions may, at least partly, explain the increasing importance in North America of PVYNTN, and to a lesser extent, PVYN-Wi [3,14,15]. Further, although synergistic interactions are generally between unrelated viruses [38,39,40] and relationships between related viruses are mostly antagonistic (competitive) [38,39], these data show that synergistic interactions do occur in very closely related PVY strains and that these interactions are genotype- and tissue-dependent. It is likely that there are host factors present in tubers with which proteins of either of the virus strains are interacting. Future studies will need to determine the mechanisms of action, including by analyzing differentially expressed genes. Such a study will determine the factors involved in PVYNTN induction of potato tuber necrotic ringspot disease (PTNRD).
It has been shown that Hc-Pro is the PVY avirulence factor corresponding to Nytbr and Ncspl [29]. The specific determinant was subsequently mapped to the Aspartic acid residue at position 419 (Asp419) in PVYO Hc-Pro [41]. In PVYN strains, there is Asp419 Glu mutation. However, breakdown of Nytbr resistance in PVYNTN and PVYN-Wi was attributed to recombination rather than accumulation of point mutations [29]. The ability of PVYNTN and PVYN-Wi to overcome resistance conferred by the Ny gene may, at least partially, explain the antagonism between these strains and PVYO. Thus, PVYNTN and PVYN-Wi overcome the resistance conferred by Ncspl and outcompete PVYO. It must be stressed that necrosis alone may not be sufficient or essential for the restriction of viral movement. Indeed, other host factors have been reported to be involved in the recognition of the avirulence protein or signaling for HR, as well as light intensity and/or quality, influencing the outcome of HR [24,25,26,27,28]. Hence, depending on the potato cultivar and environmental conditions, necrotic responses may vary phenotypically and, in addition to local necrosis, PVY may spread, causing systemic necrosis and leaf-drop in the plant, as observed on the ‘Ranger Russet’ cultivar in this study.
Epidemiologically, these data support the hypothesis that the global increase in occurrence of PVYNTN, and to a limited extent, of PVYN-Wi, is at least partially due to the ability of these strains to outcompete PVYO, the hitherto predominant strain. Thus, in mixed infection situations, the fitness cost is more severe on PVYO than on PVYNTN or PVYN-Wi. Furthermore, it has been indicated that, in potato cultivars with the Ny gene, such as ‘Ranger Russet’, the severe systemic necrosis observed in this study is beneficial in PVYO management because it leads to developmentally impaired plants, which are unlikely to be used for seed tubers [2]. Given that a large proportion of cultivated potatoes have the Ny gene [8,23,37], this results in an increasing importance of PVYNTN and PVYN-Wi at the expense of PVYO. Moreover, the fact that there were no apparent viral symptoms in the foliage of potato plants infected by PVYNTN supports the view that much of the spread is likely through tubers. Equally epidemiologically important is the fact that potato plants doubly infected by PVYN-Wi and PVYO, and by PVYN:O and PVYO, exhibited severe veinal and systemic necrosis, leading to plant death under our conditions. Thus, these plants are less likely to be used as seed tubers, thereby limiting these viral strains in mixed-infected seed tubers.
Differences in fitness have been suggested to be the driving force in mixed infections between pathogens, and underpin the natural selection process [42,43]. For viruses, fitness is the extent to which the virus adapts to the host and can produce infectious progeny (i.e., replicative fitness) [39,44,45,46]. To be infectious, the virus must uncoat and release its genome, express its genes, and replicate. Nascent viral progeny must move cell-to-cell either as the naked genome or as encapsidated particles while evading host defenses [39,47]. The ability of PVYNTN and PVYN-Wi to outcompete PVYO can at least partially be explained by the ability of the Ny gene to interfere with at least one of these stages in the virus life cycle, thereby conferring resistance. This presumably favors the replication and/or spread of PVYNTN and PVYN-Wi.
Potato seed tubers are traditionally stored at low temperatures, usually between 3 °C and 5 °C [48], and then used in the next crop. Therefore, we investigated the effect of low storage temperature on the replication and cell-to-cell movement of PVYNTN and PVYO at 4 °C and 22 °C in tubers of cultivars ‘Desiree’, ‘Ranger Russet’, and ‘Eva’. The results showed similar levels of viral RNA at both temperature regimes. Thus, unlike the pathogenicity of tuber infecting bacterial and fungal diseases, which tend to decrease at low temperatures, PVY replication and cell-to-cell movement appear not to be affected by low storage temperatures. Epidemiologically, this indicates that, even for low virus titers, at harvest, PVY can continue to replicate and move cell-to-cell in the tuber during storage. It is important to note that others have noted an unsubstantial increase in PVY levels during storage [49]. Furthermore, the fact that the higher levels of PVYNTN over PVYO were more significant at 4 °C than at 22 °C indicates that PVYNTN is much fitter during seed tuber storage and may at least partially explain its gradual replacement of PVYO in North America, where seed tubers are traditionally stored at low temperatures.
One of the most limiting factors in studying virus pathogenicity in tubers is the difficulty of carrying out reproducible transmission in tubers. Thus, here, we developed a corkborer method and used it to infect tubers of all three potato cultivars efficiently and reproducibly.
Taken together, this study indicates that the current increase in PVYNTN and PVYN-Wi incidences in potato fields, especially in North America, may be due, at least partially, to their ability to antagonize and/or outcompete other strains, especially including the prevalent PVYO strain with the increasing incorporation of the Ny resistance gene into the potato cultivars, since the interaction between PVY strains is variety-dependent [36].

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/ijpb15020032/s1, Figure S1. Amplification efficiency validation of primers used to amplify housekeeping gene and PVY strains PVYO, PVYNTN, PVYN:O, and PVYN-Wi. The Ct values were plotted against log of four different ten-fold template RNA dilutions (1, 10, 100, and 1000). This series of four dilutions was measured in two replicates per dilution. Figure S2. Interactions between PVY strains PVYO, PVYNTN, PVYN:O, and PVYN-Wi in potato cultivar ‘Ranger Russet’. singly and doubly infected (A) Interactions between PVY strains in potato cultivar ‘Ranger Russet’ at 10 dpi. (B) Interactions between PVY strains in potato cultivar ‘Ranger Russet’ at 30 dpi. Figure S3. Confirmation of specificity of PVYNTN, PVYO, PVYN:O, and PVYO primers utilized in this study as indicated by an RT-PCR amplification products run on an agarose gel. Figure S4. Typical ethidium bromide-stained Tris-borate-EDTA (TBE) agarose gel showing total potato tuber RNA extracted with our (A) guanidine hydrochloride-based buffer, and with (B) guanidine thiocyanate-based extraction buffer. The ratio of absorbance at 260 nm and 280 nm ranged from 1.90 to 2.13. Figure S5. Antagonism and synergistic interactions between PVY strains in potato tubers. PVY strains were singly- and co-inoculated to determine the effect of mixed infection on replication, which was determined using RT-qPCR. Relative viral RNA levels were determined as described in Materials and Methods. Analysis with universal PVY primers shows that except for the interaction between PVYN-Wi and PVYNTN. Figure S6. Interaction between PVY strains in tubers of ‘Russet Norkotah’, which does not carry the Ny gene. PVY strains were singly- and co-inoculated to determine the effect of mixed infection on replication, which was determined using RT-qPCR. Relative viral RNA levels were determined as described in Materials and Methods. There were significantly lower levels of PVYNTN in leaves co-inoculated with PVYO. Levels of PVYN:O were also repressed by PVYNTN and PVYN-Wi, but not by PVYO. Mean differences were determined using a t-test. Figure S7. RT-PCR test targeting the negative sense ssRNA [(−)ssRNA] of PVYNTN and PVYO Hc-Pro RNA sequence. Samples collected at 6, 24, 48, and 96 hpi and used for reverse transcription and PCR amplification. Samples from inoculated tubers showed PCR product corresponding to Hc-Pro cistron from inoculated tissues (lanes 1 to 4) of (A) PVYNTN, and (B) PVYO; lanes 5 to 8 are from samples inoculated with the phosphate buffer.

Author Contributions

Conceptualization, V.N.F. and P.M.N.; Methodology, V.N.F., P.M.N., J.A.C., H.A.C. and D.S.G.; Validation, V.N.F.; Investigation, V.N.F.; Visualization, V.N.F.; Writing, V.N.F.; Original draft, V.N.F.; Funding acquisition, V.N.F. and B.C.M.; Data Analysis V.N.F. and P.B.; Review and editing, P.B., B.C.M. and V.N.F. All authors have read and agreed to the published version of the manuscript.

Funding

This work was supported by the USDA National Institute of Food and Agriculture, Plant Biotic Interactions project number 2020-67018-31180.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The data are contained within the article.

Acknowledgments

We thank Joanna Friesner for the critical review of the manuscript.

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. Interactions between PVY strains PVYO, PVYNTN, PVYN:O, and PVYN-Wi in potato cultivar ‘Ranger Russet’. Systemically infected leaves of plants inoculated with PVYO displayed severe local veinal necrosis at 10 dpi, while leaves of plants inoculated with PVYNTN, PVYN-Wi, and PVYN:O displayed necrotic spots (A). Leaves doubly infected with PVYN-Wi and PVYO, as well as with PVYN:O and PVYO, displayed severe necrosis leading leaf death (B).
Figure 1. Interactions between PVY strains PVYO, PVYNTN, PVYN:O, and PVYN-Wi in potato cultivar ‘Ranger Russet’. Systemically infected leaves of plants inoculated with PVYO displayed severe local veinal necrosis at 10 dpi, while leaves of plants inoculated with PVYNTN, PVYN-Wi, and PVYN:O displayed necrotic spots (A). Leaves doubly infected with PVYN-Wi and PVYO, as well as with PVYN:O and PVYO, displayed severe necrosis leading leaf death (B).
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Figure 2. RT-qPCR quantification of viral RNA in leaves of potato cultivar ‘Ranger Russet’ (A) and ‘Russet Norkotah’ (B) using strain-specific primers. Viral titers were determined at 7 dpi (a–d,i–l) and 30 dpi (e–h,m–p). Each experiment had three biological replicates and three technical replicates. Details of the approach used to determine relative levels of viral RNA are provided in Materials and Methods. Mean differences were determined using a t-test (* p < 0.05; ** p < 0.01; *** p < 0.001).
Figure 2. RT-qPCR quantification of viral RNA in leaves of potato cultivar ‘Ranger Russet’ (A) and ‘Russet Norkotah’ (B) using strain-specific primers. Viral titers were determined at 7 dpi (a–d,i–l) and 30 dpi (e–h,m–p). Each experiment had three biological replicates and three technical replicates. Details of the approach used to determine relative levels of viral RNA are provided in Materials and Methods. Mean differences were determined using a t-test (* p < 0.05; ** p < 0.01; *** p < 0.001).
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Figure 3. Replication of four PVY strains, PVYO, PVYNTN, PVYN:O, and PVYN-Wi, in tubers of three potato varieties with different levels of resistance to PVY. Viral RNA levels were analyzed using RT-qPCR as described in Materials and Methods. PVYNTN, and PVYN-Wi to a lesser extent, were observed to replicate to higher levels in ‘Desiree’ and ‘Ranger Russet’ but not in Eva. Mean differences were determined using a t-test (* p < 0.05; ** p < 0.01; *** p < 0.001).
Figure 3. Replication of four PVY strains, PVYO, PVYNTN, PVYN:O, and PVYN-Wi, in tubers of three potato varieties with different levels of resistance to PVY. Viral RNA levels were analyzed using RT-qPCR as described in Materials and Methods. PVYNTN, and PVYN-Wi to a lesser extent, were observed to replicate to higher levels in ‘Desiree’ and ‘Ranger Russet’ but not in Eva. Mean differences were determined using a t-test (* p < 0.05; ** p < 0.01; *** p < 0.001).
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Figure 4. Quantification of viral RNA in potato tubers at different times (hours) after inoculation. The inoculum was added to the central well using a 1 cm corkborer and samples collected at distances from the inoculum well using a 0.5 cm corkborer and samples collected from positions a, b, and c (A) at 6, 24, 48, and 96 h post-inoculation. Viral RNA levels were analyzed using RT-qPCR as described in Materials and Methods. Tubers infected by PVYNTN and PVYO displayed lower levels of viral RNA than tubers singly infected by either of the strains, as indicated by RT-qPCR analysis using strain-specific primers (B,C). Mean differences were determined using a t-test (* p < 0.05; ** p < 0.01).
Figure 4. Quantification of viral RNA in potato tubers at different times (hours) after inoculation. The inoculum was added to the central well using a 1 cm corkborer and samples collected at distances from the inoculum well using a 0.5 cm corkborer and samples collected from positions a, b, and c (A) at 6, 24, 48, and 96 h post-inoculation. Viral RNA levels were analyzed using RT-qPCR as described in Materials and Methods. Tubers infected by PVYNTN and PVYO displayed lower levels of viral RNA than tubers singly infected by either of the strains, as indicated by RT-qPCR analysis using strain-specific primers (B,C). Mean differences were determined using a t-test (* p < 0.05; ** p < 0.01).
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Figure 5. Antagonism and synergistic interactions between PVY strains PVYO, PVYNTN, PVYN:O, and PVYN-Wi in potato tubers. PVY strains were singly and co-inoculated with (A) PVYN:O and PVYO; (B) PVYN-Wi and PVYO; (C) PVYN:O and PVYNTN; and (D) PVYN-Wi and PVYNTN, to determine the effect of mixed infection on replication, which was determined using RT-qPCR. Relative viral RNA levels were determined as described in Materials and Methods. PVYN-Wi was observed to repress PVYO and PVYNTN, but not PVYN:O, and the latter in turn represses PVYN-Wi as indicated by strain-specific primers. Mean differences were determined using a t-test (* p < 0.05).
Figure 5. Antagonism and synergistic interactions between PVY strains PVYO, PVYNTN, PVYN:O, and PVYN-Wi in potato tubers. PVY strains were singly and co-inoculated with (A) PVYN:O and PVYO; (B) PVYN-Wi and PVYO; (C) PVYN:O and PVYNTN; and (D) PVYN-Wi and PVYNTN, to determine the effect of mixed infection on replication, which was determined using RT-qPCR. Relative viral RNA levels were determined as described in Materials and Methods. PVYN-Wi was observed to repress PVYO and PVYNTN, but not PVYN:O, and the latter in turn represses PVYN-Wi as indicated by strain-specific primers. Mean differences were determined using a t-test (* p < 0.05).
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Figure 6. Effect of temperature on tuber infectivity of PVYNTN (A) and PVYO (B). Tubers of ‘Desiree’, Eva, and ‘Ranger Russet’ were inoculated and incubated at 4 °C and 22 °C, respectively. Samples were collected 6 hpi, 24 hpi, and 48 hpi along the infection gradient from the periphery at ~22 mm from the inoculation well to ~1.5 cm. Viral RNA levels were quantified using RT-qPCR as described in Materials and Methods. Mean differences were determined using a t-test test (* p < 0.05; ** p < 0.01; *** p < 0.001).
Figure 6. Effect of temperature on tuber infectivity of PVYNTN (A) and PVYO (B). Tubers of ‘Desiree’, Eva, and ‘Ranger Russet’ were inoculated and incubated at 4 °C and 22 °C, respectively. Samples were collected 6 hpi, 24 hpi, and 48 hpi along the infection gradient from the periphery at ~22 mm from the inoculation well to ~1.5 cm. Viral RNA levels were quantified using RT-qPCR as described in Materials and Methods. Mean differences were determined using a t-test test (* p < 0.05; ** p < 0.01; *** p < 0.001).
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Table 1. List of primers and targets used in this study.
Table 1. List of primers and targets used in this study.
A/RT-PCR
TargetDesignationSequence (5’-3’)Length (bp)
* PVYNTNNTN7350-F
NTN8266-R		ACATCACCGATGAGCAGG
GTACATACCCTCGATTAGCA		918
* PVYOO1962-F
O2296-R		TCAACATTCTATCCACCAAC
ACGTTTGAGTGTCATGGT		335
PVYN:ON:O1008-F
N:O1703-R		GCACGTTCCAAGGTTACC
TCGCTTAGCATGATATTCCCT		695
** PVYN-WiN-Wi_YN5-1780-F
N-Wi_YO3-2558-R		TCCGAATGGGACAAGAAAACTTG
AGGCTCATCTAACAGCAACTGTC		778
B/RT-qPCR ***
TargetDesignationSequence (5’-3’)Length (bp)
PVYNTNNTNq-6515-F
NTNq-6631-R		TCCGAGCTCCAGTGCAGAAT
AAGTGCTGCCCGGTACATTG		116
PVYOOq-4-F
Oq-138-R		CGCAAAAACACTCATAAAAGCTCA
TGGTTGGAAGTGATGAAATTGCT		134
PVYN:ONOq-6444-F
NOq-6574-R		GGATATCATCCTCATCAAATGCCG
TCGACGATGCATACTTCTCCTG		130
PVYN-WiN-Wiq-35-F
N-Wiq-156-R		TTCCTTGCAATTCTCTTAAACGGT
ACGAACCGAAACAGATTGTTGAC		121
All strains Uni-q-9426-F
Uni-q-9549-R		GTGGCAGGGTGATTTCGTCA
AGAATCGCAACATCACCTAATCG		 123
1-alpha EF1αEF1-F
EF1-R		TGGAGGCACTCCCTGGTGACA
TGTGGCAGTCGAGCACTGGT		194
* [18]; ** [19]. *** Primer pairs with efficiencies ranged from 95 to 109%.
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MDPI and ACS Style

Niraula, P.M.; Baldrich, P.; Cheema, J.A.; Cheema, H.A.; Gaiter, D.S.; Meyers, B.C.; Fondong, V.N. Antagonism and Synergism Characterize the Interactions between Four North American Potato Virus Y Strains. Int. J. Plant Biol. 2024, 15, 412-428. https://doi.org/10.3390/ijpb15020032

AMA Style

Niraula PM, Baldrich P, Cheema JA, Cheema HA, Gaiter DS, Meyers BC, Fondong VN. Antagonism and Synergism Characterize the Interactions between Four North American Potato Virus Y Strains. International Journal of Plant Biology. 2024; 15(2):412-428. https://doi.org/10.3390/ijpb15020032

Chicago/Turabian Style

Niraula, Prakash M., Patricia Baldrich, Junaid A. Cheema, Hashir A. Cheema, Dejah S. Gaiter, Blake C. Meyers, and Vincent N. Fondong. 2024. "Antagonism and Synergism Characterize the Interactions between Four North American Potato Virus Y Strains" International Journal of Plant Biology 15, no. 2: 412-428. https://doi.org/10.3390/ijpb15020032

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