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Nutrients 2013, 5(2), 455-467; doi:10.3390/nu5020455
Article

An Extract from Wax Apple (Syzygium samarangense (Blume) Merrill and Perry) Effects Glycogenesis and Glycolysis Pathways in Tumor Necrosis Factor-α-Treated FL83B Mouse Hepatocytes

1,†,* , 2,†
 and
2
1 Department of Human Development and Family Studies, National Taiwan Normal University, No. 162, Sec. 1, Heping East Road, Taipei 10610, Taiwan 2 Graduate Institute of Food Science and Technology, National Taiwan University, P.O. Box 23-14, Taipei 10672, Taiwan These two authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 29 November 2012 / Revised: 21 January 2013 / Accepted: 4 February 2013 / Published: 6 February 2013
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Abstract

FL83B mouse hepatocytes were treated with tumor necrosis factor-α (TNF-α) to induce insulin resistance to investigate the effect of a wax apple aqueous extract (WAE) in insulin-resistant mouse hepatocytes. The uptake of 2-[N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl)amino]-2-deoxyglucose (2 NBDG), a fluorescent d-glucose derivative, was performed, and the metabolism of carbohydrates was evaluated by examining the expression of glycogenesis or glycolysis-related proteins in insulin-resistant hepatocytes. The results show that WAE significantly improves the uptake of glucose and enhances glycogen content in insulin-resistant FL83B mouse hepatocytes. The results from Western blot analysis also reveal that WAE increases the expression of glycogen synthase (GS), hexokinase (HXK), glucose-6-phosphate dehydrogenase (G6PD), phosphofructokinase (PFK) and aldolase in TNF-α treated cells, indicating that WAE may ameliorate glucose metabolism by promoting glycogen synthesis and the glycolysis pathways in insulin-resistant FL83B mouse hepatocytes.
Keywords: wax apple; insulin resistance; glucose metabolism; glycogenesis; glycolysis; FL83B mouse hepatocytes wax apple; insulin resistance; glucose metabolism; glycogenesis; glycolysis; FL83B mouse hepatocytes
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Shen, S.-C.; Chang, W.-C.; Chang, C.-L. An Extract from Wax Apple (Syzygium samarangense (Blume) Merrill and Perry) Effects Glycogenesis and Glycolysis Pathways in Tumor Necrosis Factor-α-Treated FL83B Mouse Hepatocytes. Nutrients 2013, 5, 455-467.

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