DNA Hypermethylation of the Serotonin Receptor Type-2A Gene Is Associated with a Worse Response to a Weight Loss Intervention in Subjects with Metabolic Syndrome
Abstract
:1. Introduction
2. Materials and Methods
2.1. Subjects and Study Protocol
2.2. Anthropometry and Blood Pressure
2.3. Biochemical Analysis
2.4. Psychological Assessment
2.5. DNA Isolation and DNA Methylation Study
CpG ID 1 | Illumina ID | CHR Position 2 | Reference 3 |
---|---|---|---|
1 | cg15894389 | 13:47470857 | c.-688 |
2 | cg02250787 | 13:47470989 | c.-820 |
3 | cg06476131 | 13:47471052 | c.-883 |
4 | cg16188532 | 13:47471090 | c.-921 |
5 | cg09361691 | 13:47471169 | c.-1000 |
6 | cg11514288 | 13:47471197 | c.-1028 |
7 | cg27068143 | 13:47471264 | c.-1095 |
8 | cg10323433 | 13:47471562 | c.-1393 |
9 | cg02027079 | 13:47471705 | c.-1536 |
10 | cg01192538 | 13:47472050 | c.-1881 |
11 | cg01620540 | 13:47472064 | c.-1895 |
12 | cg06020661 | 13:47472138 | c.-1969 |
13 | cg09798090 | 13:47472140 | c.-1971 |
14 | cg24320398 | 13:47472158 | c.-1989 |
15 | cg18200810 | 13:47472200 | c.-2031 |
16 | cg15692052 | 13:47472250 | c.-2081 |
17 | cg24118521 | 13:47472330 | c.-2161 |
18 | cg23881368 | 13:47472343 | c.-2174 |
19 | cg05506829 | 13:47472349 | c.-2180 |
20 | cg07075299 | 13:47472360 | c.-2191 |
2.6. Analysis of Gene Expression by Quantitative Real-Time PCR
2.7. In Silico Sequence Analysis
2.8. Statistical Analyses
3. Results
Variables | Mean HTR2A Gene Methylation % | p Trend | ||
---|---|---|---|---|
Low (n = 14) | Medium (n = 14) | High (n = 13) | ||
Body weight (kg) | 97.6 ± 4.6 | 106.4 ± 4.6 | 110.7 ± 4.8 | 0.059 |
BMI (kg/m2) | 37.0 ± 1.0 | 37.0 ± 1.0 | 37.0 ± 1.0 | 0.995 |
Waist circumference (cm) | 110.8 ± 3.0 | 114.0 ± 3.0 | 120.5 ± 3.1 | 0.027 |
Total fat mass (kg) | 43.8 ± 2.4 | 45.9 ± 2.4 | 45.4 ± 2.5 | 0.682 |
Truncal fat mass (kg) | 26.1 ± 1.8 | 27.6 ± 1.8 | 26.2 ± 1.9 | 0.993 |
SBP (mmHg) | 150.3 ± 4.8 | 151.2 ± 4.8 | 148.1 ± 4.9 | 0.733 |
DBP (mmHg) | 85.4 ± 2.2 | 87.3 ± 2.2 | 84.8 ± 2.3 | 0.784 |
Glucose (mg/dL) | 9.8 ± 1.5 | 7.4 ± 1.5 | 11.1 ± 1.6 | 0.498 |
Insulin (μU/mL) | 11.0 ± 2.3 | 18.1 ± 2.3 | 21.7 ± 2.4 | 0.004 |
TC (mg/dL) | 225.1 ± 12.4 | 211.1 ± 12.9 | 205.1 ± 12.9 | 0.280 |
Triglycerides (mg/dL) | 166.8 ± 29.9 | 224.6 ± 31.1 | 247.3 ± 31.1 | 0.079 |
NEFA (nmol/L) | 0.58 ± 0.05 | 0.50 ± 0.05 | 0.56 ± 0.05 | 0.705 |
BDI score | 9.8 ± 1.9 | 8.3 ± 1.9 | 10.9 ± 1.9 | 0.636 |
Δ Body Weight | Δ WC | Δ BMI | Δ Fat Mass (kg) | ||||||
---|---|---|---|---|---|---|---|---|---|
Baseline | CpG Sites | r | Uncorrected p-Value | r | Uncorrected p-Value | r | Uncorrected p-Value | r | Uncorrected p-Value |
cg15894389 | 1 | 0.110 | 0.537 | −0.117 | 0.510 | 0.127 | 0.474 | 0.167 | 0.346 |
cg02250787 | 2 | −0.063 | 0.725 | −0.083 | 0.639 | −0.063 | 0.723 | 0.008 | 0.963 |
cg06476131 | 3 | 0.284 | 0.103 | 0.080 | 0.652 | 0.286 | 0.100 | 0.381 | 0.026 * |
cg16188532 | 4 | 0.379 | 0.027 * | 0.124 | 0.483 | 0.383 | 0.025 * | 0.415 | 0.015 * |
cg09361691 | 5 | −0.195 | 0.268 | −0.188 | 0.287 | −0.198 | 0.261 | −0.153 | 0.386 |
cg11514288 | 6 | −0.143 | 0.418 | −0.154 | 0.383 | −0.144 | 0.414 | −0.145 | 0.412 |
cg27068143 | 7 | 0.369 | 0.032 * | 0.116 | 0.513 | 0.397 | 0.020 * | 0.382 | 0.025 * |
cg10323433 | 8 | 0.292 | 0.094 | 0.052 | 0.769 | 0.350 | 0.042 | 0.249 | 0.155 |
cg02027079 | 9 | 0.011 | 0.951 | 0.160 | 0.365 | 0.009 | 0.957 | 0.042 | 0.815 |
cg01192538 | 10 | 0.225 | 0.201 | 0.193 | 0.274 | 0.230 | 0.190 | 0.214 | 0.225 |
cg01620540 | 11 | −0.070 | 0.694 | −0.251 | 0.152 | −0.014 | 0.937 | −0.073 | 0.680 |
cg06020661 | 12 | 0.177 | 0.316 | 0.206 | 0.243 | 0.196 | 0.267 | 0.196 | 0.267 |
cg09798090 | 13 | −0.042 | 0.814 | −0.054 | 0.762 | −0.037 | 0.837 | −0.059 | 0.740 |
cg24320398 | 14 | 0.338 | 0.051 | 0.266 | 0.128 | 0.377 | 0.028 * | 0.343 | 0.047 |
cg18200810 | 15 | 0.310 | 0.075 | 0.222 | 0.206 | 0.360 | 0.037 * | 0.322 | 0.063 |
cg15692052 | 16 | 0.091 | 0.608 | −0.025 | 0.890 | 0.121 | 0.494 | 0.103 | 0.563 |
cg24118521 | 17 | 0.342 | 0.047 | 0.280 | 0.108 | 0.381 | 0.026 * | 0.414 | 0.015 * |
cg23881368 | 18 | 0.297 | 0.087 | 0.269 | 0.124 | 0.319 | 0.066 | 0.291 | 0.095 |
cg05506829 | 19 | 0.280 | 0.108 | 0.252 | 0.151 | 0.297 | 0.088 | 0.268 | 0.125 |
cg07075299 | 20 | 0.296 | 0.089 | 0.250 | 0.153 | 0.307 | 0.078 | 0.301 | 0.084 |
4. Discussion
5. Conclusions
Supplementary Files
Acknowledgment
Author Contributions
Conflicts of Interest
References
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Perez-Cornago, A.; Mansego, M.L.; Zulet, M.A.; Martinez, J.A. DNA Hypermethylation of the Serotonin Receptor Type-2A Gene Is Associated with a Worse Response to a Weight Loss Intervention in Subjects with Metabolic Syndrome. Nutrients 2014, 6, 2387-2403. https://doi.org/10.3390/nu6062387
Perez-Cornago A, Mansego ML, Zulet MA, Martinez JA. DNA Hypermethylation of the Serotonin Receptor Type-2A Gene Is Associated with a Worse Response to a Weight Loss Intervention in Subjects with Metabolic Syndrome. Nutrients. 2014; 6(6):2387-2403. https://doi.org/10.3390/nu6062387
Chicago/Turabian StylePerez-Cornago, Aurora, Maria L. Mansego, María Angeles Zulet, and José Alfredo Martinez. 2014. "DNA Hypermethylation of the Serotonin Receptor Type-2A Gene Is Associated with a Worse Response to a Weight Loss Intervention in Subjects with Metabolic Syndrome" Nutrients 6, no. 6: 2387-2403. https://doi.org/10.3390/nu6062387