Bordetella Pertussis Adenylate Cyclase Toxin Does Not Possess a Phospholipase A Activity; Serine 606 and Aspartate 1079 Residues Are Not Involved in Target Cell Delivery of the Adenylyl Cyclase Enzyme Domain
Abstract
:1. Introduction
2. Results and Discussion
3. Materials and Methods
3.1. Construction, Production, and Purification of CyaA Proteins
3.2. Cell Binding, Cell Invasive, and Hemolytic Activities on Sheep Erythrocytes
3.3. Binding and cAMP Elevation of ACT on J774A.1 Cells
3.4. Phospholipase A1 Activity Determination
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Bumba, L.; Masin, J.; Osickova, A.; Osicka, R.; Sebo, P. Bordetella Pertussis Adenylate Cyclase Toxin Does Not Possess a Phospholipase A Activity; Serine 606 and Aspartate 1079 Residues Are Not Involved in Target Cell Delivery of the Adenylyl Cyclase Enzyme Domain. Toxins 2018, 10, 245. https://doi.org/10.3390/toxins10060245
Bumba L, Masin J, Osickova A, Osicka R, Sebo P. Bordetella Pertussis Adenylate Cyclase Toxin Does Not Possess a Phospholipase A Activity; Serine 606 and Aspartate 1079 Residues Are Not Involved in Target Cell Delivery of the Adenylyl Cyclase Enzyme Domain. Toxins. 2018; 10(6):245. https://doi.org/10.3390/toxins10060245
Chicago/Turabian StyleBumba, Ladislav, Jiri Masin, Adriana Osickova, Radim Osicka, and Peter Sebo. 2018. "Bordetella Pertussis Adenylate Cyclase Toxin Does Not Possess a Phospholipase A Activity; Serine 606 and Aspartate 1079 Residues Are Not Involved in Target Cell Delivery of the Adenylyl Cyclase Enzyme Domain" Toxins 10, no. 6: 245. https://doi.org/10.3390/toxins10060245