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Toxins 2013, 5(2), 327-335; doi:10.3390/toxins5020327

ESI-MS/MS Identification of a Bradykinin-Potentiating Peptide from Amazon Bothrops atrox Snake Venom Using a Hybrid Qq-oaTOF Mass Spectrometer

1
Center of Biomolecules Study Applied to Health, Fiocruz Rondônia, Oswaldo Cruz Foundation, Porto Velho, RO 76820-245, Brazil
2
MS Applications Development Laboratory, Waters Corporation, Alphaville, SP 06455-020, Brazil
3
Medicine Department, Federal University of Rondônia, Porto Velho, RO 76801-059, Brazil
*
Author to whom correspondence should be addressed.
Received: 3 September 2012 / Revised: 13 December 2012 / Accepted: 16 December 2012 / Published: 18 February 2013
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Abstract

A bradykinin-potentiating peptide (BPP) from Amazon Bothrops atrox venom with m/z 1384.7386 was identified and characterized by collision induced dissociation (CID) using an ESI-MS/MS spectra obtained in positive ion mode on a hybrid Qq-oaTOF mass spectrometer, Xevo G2 QTof MS (Waters, Manchester, UK). De novo peptide sequence analysis of the CID fragmentation spectra showed the amino acid sequence ZKWPRPGPEIPP, with a pyroglutamic acid and theoretical monoisotopic m/z 1384.7378, which is similar to experimental data, showing a mass accuracy of 0.6 ppm. The peptide is homologous to other BPP from Bothrops moojeni and was named as BPP-BAX12. View Full-Text
Keywords: bioactive peptide; BPP; pyroglutamic acid; pyrrolidonecarboxylic acid; de novo peptide sequencing bioactive peptide; BPP; pyroglutamic acid; pyrrolidonecarboxylic acid; de novo peptide sequencing
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Coutinho-Neto, A.; Caldeira, C.A.S.; Souza, G.H.M.F.; Zaqueo, K.D.; Kayano, A.M.; Silva, R.S.; Zuliani, J.P.; Soares, A.M.; Stábeli, R.G.; Calderon, L.A. ESI-MS/MS Identification of a Bradykinin-Potentiating Peptide from Amazon Bothrops atrox Snake Venom Using a Hybrid Qq-oaTOF Mass Spectrometer. Toxins 2013, 5, 327-335.

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