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Toxins 2013, 5(7), 1230-1243; doi:10.3390/toxins5071230

Fluoxetine Induced Suicidal Erythrocyte Death

1
Department of Physiology, University of Tuebingen, Gmelinstr. 5, Tuebingen 72076, Germany
2
Zentrum für Klinische Transfusionsmedizin, Otfried-Müller-Straße 4/1, Tuebingen 72076, Germany
*
Author to whom correspondence should be addressed.
Received: 23 May 2013 / Revised: 28 June 2013 / Accepted: 4 July 2013 / Published: 15 July 2013
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Abstract

The antidepressant fluoxetine inhibits ceramide producing acid sphingomyelinase. Ceramide is in turn known to trigger eryptosis the suicidal death of erythrocytes characterized by cell shrinkage and exposure of phosphatidylserine at the erythrocyte surface. Ceramide is effective through sensitizing the erythrocytes to the pro-eryptotic effect of increased cytosolic Ca2+ activity ([Ca2+]i). In nucleated cells, fluoxetine could either inhibit or stimulate suicidal death or apoptosis. The present study tested whether fluoxetine influences eryptosis. To this end cell volume was estimated from forward scatter, phosphatidylserine exposure from annexin V binding, hemolysis from hemoglobin release and [Ca2+]i from Fluo-3 fluorescence intensity. As a result, a 48 h exposure of erythrocytes to fluoxetine (≥25 µM) significantly decreased forward scatter, increased annexin V binding and enhanced [Ca2+]i. The effect on annexin V binding was significantly blunted, but not abolished, in the absence of extracellular Ca2+. In conclusion, fluoxetine stimulates eryptosis, an effect at least in part due to increase of cytosolic Ca2+ activity. View Full-Text
Keywords: phosphatidylserine; fluoxetine; calcium; cell volume; eryptosis phosphatidylserine; fluoxetine; calcium; cell volume; eryptosis
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Jilani, K.; Enkel, S.; Bissinger, R.; Almilaji, A.; Abed, M.; Lang, F. Fluoxetine Induced Suicidal Erythrocyte Death. Toxins 2013, 5, 1230-1243.

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