Identification of a Key Residue for Oligomerisation and Pore-Formation of Clostridium perfringens NetB
AbstractNecrotic enteritis toxin B (NetB) is a β-pore-forming toxin produced by Clostridium perfringens and has been identified as a key virulence factor in the pathogenesis of avian necrotic enteritis, a disease causing significant economic damage to the poultry industry worldwide. In this study, site-directed mutagenesis was used to identify amino acids that play a role in NetB oligomerisation and pore-formation. NetB K41H showed significantly reduced toxicity towards LMH cells and human red blood cells relative to wild type toxin. NetB K41H was unable to oligomerise and form pores in liposomes. These findings suggest that NetB K41H could be developed as a genetic toxoid vaccine to protect against necrotic enteritis. View Full-Text
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Fernandes da Costa, S.P.; Savva, C.G.; Bokori-Brown, M.; Naylor, C.E.; Moss, D.S.; Basak, A.K.; Titball, R.W. Identification of a Key Residue for Oligomerisation and Pore-Formation of Clostridium perfringens NetB. Toxins 2014, 6, 1049-1061.
Fernandes da Costa SP, Savva CG, Bokori-Brown M, Naylor CE, Moss DS, Basak AK, Titball RW. Identification of a Key Residue for Oligomerisation and Pore-Formation of Clostridium perfringens NetB. Toxins. 2014; 6(3):1049-1061.Chicago/Turabian Style
Fernandes da Costa, Sérgio P.; Savva, Christos G.; Bokori-Brown, Monika; Naylor, Claire E.; Moss, David S.; Basak, Ajit K.; Titball, Richard W. 2014. "Identification of a Key Residue for Oligomerisation and Pore-Formation of Clostridium perfringens NetB." Toxins 6, no. 3: 1049-1061.