Dysregulated HAI-2 Plays an Important Role in Renal Cell Carcinoma Bone Metastasis through Ligand-Dependent MET Phosphorylation
Abstract
:1. Introduction
2. Results
2.1. Expression of Each Molecule in RCC Cell Lines
2.2. Preparation of Mouse Model of Bone Metastasis
2.3. Expression of Each Molecule in Bone Metastasis
2.4. In Vitro Study Using HAI-2 Stable Knockdown and Overexpressed RCC Cells
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Animal Experiments
4.3. RNA Extraction and RT-PCR
4.4. Real-Time Quantitative PCR
4.5. Immunohistochemistry
4.6. Construction of Lentiviral Vectors and Viral Transduction into 786-O-Luc2 Cells to Produce Doxycycline-Induced HAI-2 Stable Knockdown and Overexpression Cells
4.7. Wound Healing Assay
4.8. Invasion Assay
4.9. PCR Array
4.10. Statistics
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Gene Symbol | Gene Name | 786-O-L2898 Fold Change | 786-O-L2853 Fold Change |
---|---|---|---|
CCL7 | Chemokine (C-C motif) ligand 7 | 4.29 | 1.33 |
CDH1 | Cadherin 1, type 1, E-cadherin | −1.08 | 1.72 |
CXCR4 | Chemokine (C-X-C motif) receptor 4 | 2.57 | −2.45 |
MMP9 | Matrix metalloprotease 9 | 1.69 | −7.29 |
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Yamasaki, K.; Mukai, S.; Sugie, S.; Nagai, T.; Nakahara, K.; Kamibeppu, T.; Sakamoto, H.; Shibasaki, N.; Terada, N.; Toda, Y.; et al. Dysregulated HAI-2 Plays an Important Role in Renal Cell Carcinoma Bone Metastasis through Ligand-Dependent MET Phosphorylation. Cancers 2018, 10, 190. https://doi.org/10.3390/cancers10060190
Yamasaki K, Mukai S, Sugie S, Nagai T, Nakahara K, Kamibeppu T, Sakamoto H, Shibasaki N, Terada N, Toda Y, et al. Dysregulated HAI-2 Plays an Important Role in Renal Cell Carcinoma Bone Metastasis through Ligand-Dependent MET Phosphorylation. Cancers. 2018; 10(6):190. https://doi.org/10.3390/cancers10060190
Chicago/Turabian StyleYamasaki, Koji, Shoichiro Mukai, Satoru Sugie, Takahiro Nagai, Kozue Nakahara, Toyoharu Kamibeppu, Hiromasa Sakamoto, Noboru Shibasaki, Naoki Terada, Yoshinobu Toda, and et al. 2018. "Dysregulated HAI-2 Plays an Important Role in Renal Cell Carcinoma Bone Metastasis through Ligand-Dependent MET Phosphorylation" Cancers 10, no. 6: 190. https://doi.org/10.3390/cancers10060190