Beta-Hydroxybutyrate Augments Oxaliplatin-Induced Cytotoxicity by Altering Energy Metabolism in Colorectal Cancer Organoids
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Establishment of Healthy and CRC Organoid Cultures
2.2. Investigating Therapeutic Interventions and Viability in Organoid Models
2.3. Protein Analysis via Western Blot
2.4. Assessment of ROS Levels
2.5. Statistical Analysis
3. Results
3.1. Ascertaining Doses for Usage of BOHB, Melatonin, and Oxaliplatin on Organoids
3.2. BOHB Enhances Oxaliplatin Treatment Efficacy in CRC Organoids
3.3. BOHB Modifies Energy Metabolism Pathways
3.4. Beta Hydroxybutyrate Does Not Influence Histone Acetylation-Dependent Apoptosis
3.5. Melatonin Shields Colorectal Cancer Cells from ROS-Induced Apoptosis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sever, T.; Ellidokuz, E.B.; Basbinar, Y.; Ellidokuz, H.; Yilmaz, Ö.H.; Calibasi-Kocal, G. Beta-Hydroxybutyrate Augments Oxaliplatin-Induced Cytotoxicity by Altering Energy Metabolism in Colorectal Cancer Organoids. Cancers 2023, 15, 5724. https://doi.org/10.3390/cancers15245724
Sever T, Ellidokuz EB, Basbinar Y, Ellidokuz H, Yilmaz ÖH, Calibasi-Kocal G. Beta-Hydroxybutyrate Augments Oxaliplatin-Induced Cytotoxicity by Altering Energy Metabolism in Colorectal Cancer Organoids. Cancers. 2023; 15(24):5724. https://doi.org/10.3390/cancers15245724
Chicago/Turabian StyleSever, Tolga, Ender Berat Ellidokuz, Yasemin Basbinar, Hulya Ellidokuz, Ömer H. Yilmaz, and Gizem Calibasi-Kocal. 2023. "Beta-Hydroxybutyrate Augments Oxaliplatin-Induced Cytotoxicity by Altering Energy Metabolism in Colorectal Cancer Organoids" Cancers 15, no. 24: 5724. https://doi.org/10.3390/cancers15245724