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Catalysts 2017, 7(10), 296; doi:10.3390/catal7100296

Immobilization of Pyrroloquinoline Quinone-Dependent Alcohol Dehydrogenase with a Polyion Complex and Redox Polymer for a Bioanode

Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo 184-8588, Japan
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Received: 28 August 2017 / Revised: 28 September 2017 / Accepted: 30 September 2017 / Published: 3 October 2017
(This article belongs to the Special Issue Biocatalysis and Biotransformations)
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Abstract

A bioanode for ethanol oxidation was prepared by immobilizing the recombinant pyrroloquinoline quinone (PQQ)-dependent alcohol dehydrogenase from Pseudomonas putida KT 2440 (PpADH) with polyion complex (PIC) and redox polymer. The PIC based on poly-l-lysine (PLL) and poly-l-glutamic acid (PGA) was suitable for immobilizing PpADH on the electrode. PpADH was immobilized using only one redox polymer, aminoferrocene, which was attached to the PGA backbone (PGA-AmFc) on the electrode. The anodic current density at 0.6 V (vs. Ag/AgCl) was 22.6 μA·cm−2. However, when the number of the cycles was increased, the catalytic current drastically decreased. PpADH was immobilized using PGA-AmFc and PIC on the electrode. The anodic current density at 0.5 V (vs. Ag/AgCl) was 47.3 μA·cm−2, and the performance maintained 74% of the initial value after five cycles. This result indicated that the combination of PIC and PGA-AmFc was suitable for the immobilization of PpADH on the electrode. In addition, the long-term stability and catalytic current density were improved by using the large surface area afforded by the gold nanoparticles. View Full-Text
Keywords: biofuel cells; quinoproteins; pyrroloquinoline quinone; alcohol dehydrogenase; polyion complex; redox mediator; gold nanoparticles biofuel cells; quinoproteins; pyrroloquinoline quinone; alcohol dehydrogenase; polyion complex; redox mediator; gold nanoparticles
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Sakurada, Y.; Takeda, K.; Ohno, H.; Nakamura, N. Immobilization of Pyrroloquinoline Quinone-Dependent Alcohol Dehydrogenase with a Polyion Complex and Redox Polymer for a Bioanode. Catalysts 2017, 7, 296.

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