Mechanical and Biological Characterization of PMMA/Al2O3 Composites for Dental Implant Abutments
Abstract
:1. Introduction
2. Materials and Methods
2.1. Material
2.2. Preparation of the PMMA/Al2O3 Composites
2.3. Morphological Analysis
2.4. WAXD Analysis
2.5. Mechanical Properties
2.6. Surface Roughness and Wettability
2.7. Protein Adsorption Assay
2.8. Cell Culture
2.9. Cell Adhesion and Viability Assays
2.10. Cell Morphology and Orientation Analysis
2.11. Real-Time qRT-PCR
2.12. Statistical Analysis
3. Results
3.1. Morphological Analysis
3.2. Structural Analysis
3.3. Mechanical Properties
3.4. Surface Properties (Wettability and Roughness)
3.5. Protein Adsorption
3.6. Cell Adhesion and Viability
3.7. Cell morphology and Orientation Analysis
3.8. Alumina Filler Decreases Osteogenic Genes and Mineralization
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Hardness (Shore D) | Flexural Modulus (GPa) | Flexural Strength (MPa) | Elongation at Break (%) | Water Contact Angle (°) | Roughness (µm) | |
---|---|---|---|---|---|---|
PMMA | 91.8 ± 1.5 | 3.86 ± 0.80 | 104.2 ± 15.5 | 3.21 ± 0.63 | 77.2 ± 3.8 | 1.94 ± 0.33 |
PMMA/Al2O3 70/30 | 96.8 ± 1.2 | 4.39 ± 0.26 | 80.5 ± 4.6 | 2.04 ± 0.12 | 99.7 ± 5.3 | 2.28 ± 0.35 |
PMMA/Al2O3 60/40 | 96.8 ± 1.6 | 4.50 ± 0.13 | 72.1 ± 3.7 | 1.78 ± 0.06 | 103.6 ± 10.0 | 2.32 ± 0.39 |
PMMA/Al2O3 50/50 | 97.0 ± 1.2 | 4.44 ± 0.13 | 65.4 ± 3.2 | 1.64 ± 0.08 | 105.8 ± 4.6 | 2.45 ± 0.44 |
Row | Mean | R | S | Rayleigh_Pval | Vtest_Pval |
---|---|---|---|---|---|
PMMA_0A_1_1 | 35.67701600 | 0.072035935 | 0.927964065 | 0.474441214 | 0.110761126 |
PMMA_0A_1_2 | 50.63379808 | 0.088506355 | 0.911493645 | 0.721981796 | 0.208632835 |
PMMA_0A_1_3 | 52.86819961 | 0.092353823 | 0.907646177 | 0.701346336 | 0.198654567 |
PMMA_0A_24_1 | 82.74359410 | 0.118870528 | 0.881129472 | 0.352655849 | 0.074071439 |
PMMA_0A_24_2 | 42.39168138 | 0.042559193 | 0.957440807 | 0.921683113 | 0.342375407 |
PMMA_0A_24_3 | 73.89992077 | 0.179232651 | 0.820767349 | 0.136471136 | 0.022975108 |
PMMA_30A_1_1 | −61.48795269 | 0.025929487 | 0.974070513 | 0.965958569 | 0.395724331 |
PMMA_30A_1_2 | −68.56930945 | 0.008236939 | 0.991763061 | 0.996173616 | 0.464959623 |
PMMA_30A_1_3 | 86.88319288 | 0.017617649 | 0.982382351 | 0.927777572 | 0.349160372 |
PMMA_30A_24_1 | 28.58235944 | 0.066870283 | 0.933129717 | 0.527206483 | 0.128637695 |
PMMA_30A_24_2 | 26.84561799 | 0.067942743 | 0.932057257 | 0.548369580 | 0.136178979 |
PMMA_30A_24_3 | 43.90423995 | 0.092727566 | 0.907272434 | 0.625224085 | 0.165392251 |
PMMA_40A_1_1 | −1214876817 | 0.199050932 | 0.800949068 | 0.002521487 | 0.000282729 |
PMMA_40A_1_2 | −26.82576711 | 0.245658642 | 0.754341358 | 0.021715219 | 0.002912159 |
PMMA_40A_1_3 | −37.88083450 | 0.251121755 | 0.748878245 | 0.038089695 | 0.005407698 |
PMMA_40A_24_1 | 72.53467140 | 0.375807448 | 0.624192552 | 7.21462 × 10−7 | 8.27644 × 10−8 |
PMMA_40A_24_2 | 79.77644706 | 0.333230014 | 0.666769986 | 0.000127143 | 1.40318 × 10−5 |
PMMA_40A_24_3 | 60.54074263 | 0.166938425 | 0.833061575 | 0.097362791 | 0.015490374 |
PMMA_40A_24_4 | 27.69148361 | 0.372972822 | 0.627027178 | 5.90978 × 10−5 | 6.82014 × 10−6 |
PMMA_50A_1_1 | −4.806702539 | 0.088296361 | 0.911703639 | 0.663567024 | 0.181657465 |
PMMA_50A_1_2 | −17.28843119 | 0.087947123 | 0.912052877 | 0.647910275 | 0.174922305 |
PMMA_50A_1_3 | −11.75231262 | 0.081283560 | 0.918716440 | 0.337785021 | 0.070193658 |
PMMA_50A_24_1 | −61.55226548 | 0.235267368 | 0.764732632 | 0.005880955 | 0.000708117 |
PMMA_50A_24_2 | 73.61116062 | 0.225430377 | 0.774569623 | 0.006611798 | 0.000799675 |
PMMA_50A_24_3 | 73.74353041 | 0.198685652 | 0.801314348 | 0.008193347 | 0.000998012 |
PMMA_50A_24_4 | 61.23645716 | 0.274637545 | 0.725362455 | 0.001507763 | 0.000171249 |
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Roato, I.; Genova, T.; Duraccio, D.; Ruffinatti, F.A.; Zanin Venturini, D.; Di Maro, M.; Mosca Balma, A.; Pedraza, R.; Petrillo, S.; Chinigò, G.; et al. Mechanical and Biological Characterization of PMMA/Al2O3 Composites for Dental Implant Abutments. Polymers 2023, 15, 3186. https://doi.org/10.3390/polym15153186
Roato I, Genova T, Duraccio D, Ruffinatti FA, Zanin Venturini D, Di Maro M, Mosca Balma A, Pedraza R, Petrillo S, Chinigò G, et al. Mechanical and Biological Characterization of PMMA/Al2O3 Composites for Dental Implant Abutments. Polymers. 2023; 15(15):3186. https://doi.org/10.3390/polym15153186
Chicago/Turabian StyleRoato, Ilaria, Tullio Genova, Donatella Duraccio, Federico Alessandro Ruffinatti, Diletta Zanin Venturini, Mattia Di Maro, Alessandro Mosca Balma, Riccardo Pedraza, Sara Petrillo, Giorgia Chinigò, and et al. 2023. "Mechanical and Biological Characterization of PMMA/Al2O3 Composites for Dental Implant Abutments" Polymers 15, no. 15: 3186. https://doi.org/10.3390/polym15153186