Lineage-Selective Disturbance of Early Human Hematopoietic Progenitor Cell Differentiation by the Commonly Used Plasticizer Di-2-ethylhexyl Phthalate via Reactive Oxygen Species: Fatty Acid Oxidation Makes the Difference
Abstract
:1. Introduction
2. Materials and Methods
2.1. Human CD34+ Cell Culture
2.2. HUVEC and HepG2/C3A Cell Culture
2.3. Flow Cytometry
2.4. Caspase 3/7 Activation
2.5. ATP Quantification
2.6. NADPH Quantification
2.7. H2DCFDC-Assay for Detection of ROS
2.8. Isolation of Total RNA and qPCR
2.9. LC-MS-Based Metabolomics
2.10. Western Blot Analysis
2.11. Measurement of Superoxide Dismutase (SOD) Activity
2.12. GC-MS-Based Quantification of DEHP and MEHP in Culture Medium
2.13. Quantification and Statistical Analysis
3. Results
3.1. DEHP Induces Erythrocyte and Dendritic Cell Apoptosis While Enhancing Neutrophil Maturation
3.2. DEHP Alters the Lipidome Composition and Reduces the Rates of Glycolysis, Glutaminolysis, and Polyamine Synthesis in Erythrocytes and Dendritic Cells
3.3. MEHP, the First Metabolite of DEHP, Was Detected at Environmentally Relevant Concentrations and Induced the Accumulation of ROS via the Reduction of SOD Activity
3.4. MEHP Induces a ROS-Mediated Shift from Glycolysis to the Pentose Phosphate Pathway, Resulting in Cell Death in Lineages without Active FAO
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Kaiser, L.; Quint, I.; Csuk, R.; Jung, M.; Deigner, H.-P. Lineage-Selective Disturbance of Early Human Hematopoietic Progenitor Cell Differentiation by the Commonly Used Plasticizer Di-2-ethylhexyl Phthalate via Reactive Oxygen Species: Fatty Acid Oxidation Makes the Difference. Cells 2021, 10, 2703. https://doi.org/10.3390/cells10102703
Kaiser L, Quint I, Csuk R, Jung M, Deigner H-P. Lineage-Selective Disturbance of Early Human Hematopoietic Progenitor Cell Differentiation by the Commonly Used Plasticizer Di-2-ethylhexyl Phthalate via Reactive Oxygen Species: Fatty Acid Oxidation Makes the Difference. Cells. 2021; 10(10):2703. https://doi.org/10.3390/cells10102703
Chicago/Turabian StyleKaiser, Lars, Isabel Quint, René Csuk, Manfred Jung, and Hans-Peter Deigner. 2021. "Lineage-Selective Disturbance of Early Human Hematopoietic Progenitor Cell Differentiation by the Commonly Used Plasticizer Di-2-ethylhexyl Phthalate via Reactive Oxygen Species: Fatty Acid Oxidation Makes the Difference" Cells 10, no. 10: 2703. https://doi.org/10.3390/cells10102703