USP10 Contributes to Colon Carcinogenesis via mTOR/S6K Mediated HIF-1α but Not HIF-2α Protein Synthesis
Abstract
:1. Introduction
2. Materials and Methods
3. Results
3.1. Hypoxia Reduces USP10 mRNA and Protein Levels
3.2. USP10 Knockout Elevates HIF-1α but Not HIF-2α Protein Levels
3.3. USP10 Deficiency Affects HIF-1α Signaling and Transcriptional Activity
3.4. USP10 Deficiency Increases S6 Kinase Activity and HIF-1α Synthesis
3.5. USP10 Knockout Affects the Cellular Energy Phenotype and Induces Glycolysis
3.6. HIF Knockdown Rescues USP10-Dependent Migration and Adhesion but Not Proliferation
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Kubaichuk, K.; Kietzmann, T. USP10 Contributes to Colon Carcinogenesis via mTOR/S6K Mediated HIF-1α but Not HIF-2α Protein Synthesis. Cells 2023, 12, 1585. https://doi.org/10.3390/cells12121585
Kubaichuk K, Kietzmann T. USP10 Contributes to Colon Carcinogenesis via mTOR/S6K Mediated HIF-1α but Not HIF-2α Protein Synthesis. Cells. 2023; 12(12):1585. https://doi.org/10.3390/cells12121585
Chicago/Turabian StyleKubaichuk, Kateryna, and Thomas Kietzmann. 2023. "USP10 Contributes to Colon Carcinogenesis via mTOR/S6K Mediated HIF-1α but Not HIF-2α Protein Synthesis" Cells 12, no. 12: 1585. https://doi.org/10.3390/cells12121585